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Infect Immun, July 1998, p. 3436-3442, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of MGC2, a Mycoplasma gallisepticum Cytadhesin with Homology to the Mycoplasma pneumoniae 30-Kilodalton Protein P30 and Mycoplasma genitalium P32dagger

Linda L. Hnatow,1 Calvin L. Keeler Jr.,1 Laura L. Tessmer,1 Kirk Czymmek,2 and John E. Dohms1,*

Department of Animal and Food Sciences, College of Agriculture and Natural Resources,1 and Department of Biology, College of Arts and Sciences,2 University of Delaware, Newark, Delaware 19717-1303

Received 8 December 1997/Returned for modification 28 January 1998/Accepted 21 April 1998

A second cytadhesin-like protein, MGC2, was identified in the avian respiratory pathogen Mycoplasma gallisepticum. The 912-nucleotide mgc2 gene encodes a 32.6-kDa protein with 40.9 and 31.4% identity with the M. pneumoniae P30 and M. genitalium P32 cytadhesins, respectively. Functional studies with reverse transcription-PCR, immunoblotting, double-sided immunogold labeling, and attachment inhibition assays demonstrated homology to the human mycoplasmal P30 and P32 cytadhesins. These findings suggest that there is a family of cytadhesin genes conserved among pathogenic mycoplasmas infecting widely divergent hosts.

* Corresponding author. Mailing address: Department of Animal and Food Sciences, University of Delaware, Newark, DE 19717-1303. Phone: (302) 831-2524. Fax: (302) 831-3651. E-mail: John.Dohms{at}mvs.udel.edu.

dagger Paper no. 1645 in the Journal Series of the Delaware Agricultural Experiment Station.

Infect Immun, July 1998, p. 3436-3442, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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