This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fleiszig, S. M. J.
Right arrow Articles by Mostov, K. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fleiszig, S. M. J.
Right arrow Articles by Mostov, K. E.

 Previous Article  |  Next Article 

Infect Immun, July 1998, p. 3443-3446, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Susceptibility of Epithelial Cells to Pseudomonas aeruginosa Invasion and Cytotoxicity Is Upregulated by Hepatocyte Growth Factor

Suzanne M. J. Fleiszig,1,* Vicky Vallas,1 Cindy H. Jun,1 Leo Mok,1 Daniel F. Balkovetz,2,dagger Michael G. Roth,3 and Keith E. Mostov2

The Morton D. Sarver Laboratory for Cornea and Contact Lens Research, School of Optometry, University of California, Berkeley,1 and Department of Anatomy and Biochemistry, University of California, San Francisco,2 California, and University of Texas Southwestern Medical Center at Dallas, Dallas, Texas3

Received 6 March 1998/Accepted 16 April 1998

Normal cell polarity protects epithelial cells against Pseudomonas aeruginosa invasion and cytotoxicity. Using epithelial cell clones with selective defects in sorting of membrane constituents, and using hepatocyte growth factor pretreatment, we found that polarized susceptibility to P. aeruginosa can be altered without disrupting tight junctions. The results also showed that cellular susceptibility factors for invasion and cytotoxicity are not the same, although both are localized to the basolateral cell surface in polarized epithelial cells.

* Corresponding author. Mailing address: School of Optometry, University of California, Berkeley, CA 94720-2020. Phone: (510) 643-0990. Fax: (510) 643-5109. E-mail: fleiszig{at}socrates.berkeley.edu.

dagger Present address: Departments of Medicine and Cell Biology, University of Alabama at Birmingham and Department of Veterans Affairs, Birmingham Veterans Affairs Medical Center, Birmingham, Alabama.

Infect Immun, July 1998, p. 3443-3446, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kierbel, A., Gassama-Diagne, A., Mostov, K., Engel, J. N. (2005). The Phosphoinositol-3-Kinase-Protein Kinase B/Akt Pathway Is Critical for Pseudomonas aeruginosa Strain PAK Internalization. Mol. Biol. Cell 16: 2577-2585 [Abstract] [Full Text]  
  • Balkovetz, D. F., Gerrard, E. R. Jr., Li, S., Johnson, D., Lee, J., Tobias, J. W., Rogers, K. K., Snyder, R. W., Lipschutz, J. H. (2004). Gene expression alterations during HGF-induced dedifferentiation of a renal tubular epithelial cell line (MDCK) using a novel canine DNA microarray. Am. J. Physiol. Renal Physiol. 286: F702-F710 [Abstract] [Full Text]  
  • Kazmierczak, B. I., Mostov, K., Engel, J. N. (2004). Epithelial Cell Polarity Alters Rho-GTPase Responses to Pseudomonas aeruginosa. Mol. Biol. Cell 15: 411-419 [Abstract] [Full Text]  
  • Fleiszig, S. M. J., Kwong, M. S. F., Evans, D. J. (2003). Modification of Pseudomonas aeruginosa Interactions with Corneal Epithelial Cells by Human Tear Fluid. Infect. Immun. 71: 3866-3874 [Abstract] [Full Text]  
  • Priebe, G. P., Meluleni, G. J., Coleman, F. T., Goldberg, J. B., Pier, G. B. (2003). Protection against Fatal Pseudomonas aeruginosa Pneumonia in Mice after Nasal Immunization with a Live, Attenuated aroA Deletion Mutant. Infect. Immun. 71: 1453-1461 [Abstract] [Full Text]  
  • Priebe, G. P., Brinig, M. M., Hatano, K., Grout, M., Coleman, F. T., Pier, G. B., Goldberg, J. B. (2002). Construction and Characterization of a Live, Attenuated aroA Deletion Mutant of Pseudomonas aeruginosa as a Candidate Intranasal Vaccine. Infect. Immun. 70: 1507-1517 [Abstract] [Full Text]  
  • Chroneos, Z. C., Wert, S. E., Livingston, J. L., Hassett, D. J., Whitsett, J. A. (2000). Role of Cystic Fibrosis Transmembrane Conductance Regulator in Pulmonary Clearance of Pseudomonas aeruginosa In Vivo. J. Immunol. 165: 3941-3950 [Abstract] [Full Text]  
  • Comolli, J. C., Waite, L. L., Mostov, K. E., Engel, J. N. (1999). Pili Binding to Asialo-GM1 on Epithelial Cells Can Mediate Cytotoxicity or Bacterial Internalization by Pseudomonas aeruginosa. Infect. Immun. 67: 3207-3214 [Abstract] [Full Text]  
  • Lee, A., Chow, D., Haus, B., Tseng, W., Evans, D., Fleiszig, S., Chandy, G., Machen, T. (1999). Airway epithelial tight junctions and binding and cytotoxicity of Pseudomonas aeruginosa. Am. J. Physiol. Lung Cell. Mol. Physiol. 277: L204-L217 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»