This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mehock, J. R.
Right arrow Articles by Krause, D. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mehock, J. R.
Right arrow Articles by Krause, D. C.

 Previous Article  |  Next Article 

Infect Immun, July 1998, p. 3462-3466, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Bartonella henselae Invasion of Feline Erythrocytes In Vitro

Jane R. Mehock,1 Craig E. Greene,2 Frank C. Gherardini,1 Tae-Wook Hahn,1 and Duncan C. Krause1,*

Departments of Microbiology1 and Small Animal Medicine,2 University of Georgia, Athens, Georgia 30602

Received 22 December 1997/Returned for modification 3 February 1998/Accepted 31 March 1998

Bartonella henselae, the causative agent of cat scratch disease, establishes long-term bacteremia in cats, in which it attaches to and invades feline erythrocytes (RBC). Feline RBC invasion was assessed in vitro, based on gentamicin selection for intracellular bacteria or by laser confocal microscopy and digital sectioning. Invasion rates ranged from 2 to 20% of the inoculum, corresponding to infection of less than 1% of the RBC. Invasion was a slow process, requiring >8 h before significant numbers of intracellular bacteria were detected. Pretreatment of the bacteria with trypsin, or of the RBC with trypsin or neuraminidase, had no effect, but pronase pretreatment of RBC resulted in a slight increase in invasion frequency. The ability to model B. henselae invasion of feline RBC in vitro should permit identification of bacterial surface components involved in this process and elucidate the significance of RBC invasion to transmission and infection in cats.

* Corresponding author. Mailing address: Department of Microbiology, 523 Biological Sciences Building, University of Georgia, Athens, GA 30602. Phone: (706) 542-2671. Fax: (706) 542-2674. E-mail: DKRAUSE{at}ARCHES.UGA.EDU.

Infect Immun, July 1998, p. 3462-3466, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Groebel, K., Hoelzle, K., Wittenbrink, M. M., Ziegler, U., Hoelzle, L. E. (2009). Mycoplasma suis Invades Porcine Erythrocytes. Infect. Immun. 77: 576-584 [Abstract] [Full Text]  
  • Mandle, T., Einsele, H., Schaller, M., Neumann, D., Vogel, W., Autenrieth, I. B., Kempf, V. A. J. (2005). Infection of human CD34+ progenitor cells with Bartonella henselae results in intraerythrocytic presence of B henselae. Blood 106: 1215-1222 [Abstract] [Full Text]  
  • Chenoweth, M. R., Greene, C. E., Krause, D. C., Gherardini, F. C. (2004). Predominant Outer Membrane Antigens of Bartonella henselae. Infect. Immun. 72: 3097-3105 [Abstract] [Full Text]  
  • Rolain, J.-M., Locatelli, C., Chabanne, L., Davoust, B., Raoult, D. (2004). Prevalence of Bartonella clarridgeiae and Bartonella henselae in Domestic Cats from France and Detection of the Organisms in Erythrocytes by Immunofluorescence. CVI 11: 423-425 [Abstract] [Full Text]  
  • Rolain, J.-M., Maurin, M., Mallet, M.-N., Parzy, D., Raoult, D. (2003). Culture and Antibiotic Susceptibility of Bartonella quintana in Human Erythrocytes. Antimicrob. Agents Chemother. 47: 614-619 [Abstract] [Full Text]  
  • GREUB, G., RAOULT, D. (2002). Bartonella: new explanations for old diseases. J Med Microbiol 51: 915-923 [Full Text]  
  • Rolain, J. M., La Scola, B., Liang, Z., Davoust, B., Raoult, D. (2001). Immunofluorescent Detection of Intraerythrocytic Bartonella henselae in Naturally Infected Cats. J. Clin. Microbiol. 39: 2978-2980 [Abstract] [Full Text]  
  • Coleman, S. A., Minnick, M. F. (2001). Establishing a Direct Role for the Bartonella bacilliformis Invasion-Associated Locus B (IalB) Protein in Human Erythrocyte Parasitism. Infect. Immun. 69: 4373-4381 [Abstract] [Full Text]  
  • O'Reilly, K. L., Parr, K. A., Brown, T. P., Tedder-Ferguson, B., Scholl, D. T. (2001). Passive Antibody to Bartonella henselae Protects against Clinical Disease following Homologous Challenge but Does Not Prevent Bacteremia in Cats. Infect. Immun. 69: 1880-1882 [Abstract] [Full Text]  
  • Munana, K. R., Vitek, S. M., Hegarty, B. C., Kordick, D. L., Breitschwerdt, E. B. (2001). Infection of Fetal Feline Brain Cells in Culture with Bartonella henselae. Infect. Immun. 69: 564-569 [Abstract] [Full Text]  
  • Breitschwerdt, E. B., Kordick, D. L. (2000). Bartonella Infection in Animals: Carriership, Reservoir Potential, Pathogenicity, and Zoonotic Potential for Human Infection. Clin. Microbiol. Rev. 13: 428-438 [Abstract] [Full Text]  
  • O'Reilly, K. L., Bauer, R. W., Freeland, R. L., Foil, L. D., Hughes, K. J., Rohde, K. R., Roy, A. F., Stout, R. W., Triche, P. C. (1999). Acute Clinical Disease in Cats following Infection with a Pathogenic Strain of Bartonella henselae (LSU16). Infect. Immun. 67: 3066-3072 [Abstract] [Full Text]  
  • Kordick, D. L., Brown, T. T., Shin, K., Breitschwerdt, E. B. (1999). Clinical and Pathologic Evaluation of Chronic Bartonella henselae or Bartonella clarridgeiae Infection in Cats. J. Clin. Microbiol. 37: 1536-1547 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»