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Infect Immun, August 1998, p. 3492-3500, Vol. 66, No. 8
Department of TB Immunology,
Received 26 January 1998/Returned for modification 24 March
1998/Accepted 5 May 1998
Culture filtrate from Mycobacterium tuberculosis
contains molecules which promote high levels of protective immunity in
animal models of subunit vaccination against tuberculosis. We have used two-dimensional electrophoresis for analysis and purification of six
novel M. tuberculosis culture filtrate proteins (CFPs): CFP17, CFP20, CFP21, CFP22, CFP25, and CFP28. The proteins were tested
for recognition by M. tuberculosis-reactive memory cells from different strains of inbred mice and for their capacity to induce
a skin test response in M. tuberculosis-infected guinea pigs. CFP17, CFP20, CFP21 and CFP25 induced both a high gamma interferon release and a strong delayed-type hypersensitivity response,
and CFP21 was broadly recognized by different strains of inbred mice.
N-terminal sequences were obtained for the six proteins, and the
corresponding genes were identified in the Sanger M. tuberculosis genome database. In parallel we established a two-dimensional electrophoresis reference map of short-term culture filtrate components and mapped novel proteins as well as already-known CFP.
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Two-Dimensional Electrophoresis for Analysis of
Mycobacterium tuberculosis Culture Filtrate and Purification
and Characterization of Six Novel Proteins
*
Corresponding author. Mailing address: Department of TB
Immunology, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen S, Denmark. Phone: 45 32 68 34 62. Fax: 45 32 68 30 35. E-mail: tbimm{at}ssi.dk.
Infect Immun, August 1998, p. 3492-3500, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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