This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weldingh, K.
Right arrow Articles by Andersen, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weldingh, K.
Right arrow Articles by Andersen, P.

 Previous Article  |  Next Article 

Infect Immun, August 1998, p. 3492-3500, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Two-Dimensional Electrophoresis for Analysis of Mycobacterium tuberculosis Culture Filtrate and Purification and Characterization of Six Novel Proteins

Karin Weldingh,1 Ida Rosenkrands,1 Susanne Jacobsen,2 Peter Birk Rasmussen,1 Martin J. Elhay,1 and Peter Andersen1 *

Department of TB Immunology, Statens Serum Institut, Copenhagen,1 and Department of Biochemistry and Nutrition, Technical University of Denmark, Lyngby,2 Denmark

Received 26 January 1998/Returned for modification 24 March 1998/Accepted 5 May 1998

Culture filtrate from Mycobacterium tuberculosis contains molecules which promote high levels of protective immunity in animal models of subunit vaccination against tuberculosis. We have used two-dimensional electrophoresis for analysis and purification of six novel M. tuberculosis culture filtrate proteins (CFPs): CFP17, CFP20, CFP21, CFP22, CFP25, and CFP28. The proteins were tested for recognition by M. tuberculosis-reactive memory cells from different strains of inbred mice and for their capacity to induce a skin test response in M. tuberculosis-infected guinea pigs. CFP17, CFP20, CFP21 and CFP25 induced both a high gamma interferon release and a strong delayed-type hypersensitivity response, and CFP21 was broadly recognized by different strains of inbred mice. N-terminal sequences were obtained for the six proteins, and the corresponding genes were identified in the Sanger M. tuberculosis genome database. In parallel we established a two-dimensional electrophoresis reference map of short-term culture filtrate components and mapped novel proteins as well as already-known CFP.

* Corresponding author. Mailing address: Department of TB Immunology, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen S, Denmark. Phone: 45 32 68 34 62. Fax: 45 32 68 30 35. E-mail: tbimm{at}ssi.dk.

Infect Immun, August 1998, p. 3492-3500, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Tiwari, D., Singh, R. K., Goswami, K., Verma, S. K., Prakash, B., Nandicoori, V. K. (2009). Key Residues in Mycobacterium tuberculosis Protein Kinase G Play a Role in Regulating Kinase Activity and Survival in the Host. J. Biol. Chem. 284: 27467-27479 [Abstract] [Full Text]  
  • West, N. P., Chow, F. M. E., Randall, E. J., Wu, J., Chen, J., Ribeiro, J. M. C., Britton, W. J. (2009). Cutinase-like proteins of Mycobacterium tuberculosis: characterization of their variable enzymatic functions and active site identification. FASEB J. 23: 1694-1704 [Abstract] [Full Text]  
  • Fu, R., Wang, C., Shi, C., Lu, M., Fang, Z., Lu, J., Wang, F., Fan, X. (2009). An Improved Whole-Blood Gamma Interferon Assay Based on the CFP21-MPT64 Fusion Protein. CVI 16: 686-691 [Abstract] [Full Text]  
  • Parker, S. K., Curtin, K. M., Vasil, M. L. (2007). Purification and Characterization of Mycobacterial Phospholipase A: an Activity Associated with Mycobacterial Cutinase. J. Bacteriol. 189: 4153-4160 [Abstract] [Full Text]  
  • Warner, D. F., Mizrahi, V. (2006). Tuberculosis Chemotherapy: the Influence of Bacillary Stress and Damage Response Pathways on Drug Efficacy. Clin. Microbiol. Rev. 19: 558-570 [Abstract] [Full Text]  
  • Sable, S. B., Kumar, R., Kalra, M., Verma, I., Khuller, G. K., Dobos, K., Belisle, J. T. (2005). Peripheral Blood and Pleural Fluid Mononuclear Cell Responses to Low-Molecular-Mass Secretory Polypeptides of Mycobacterium tuberculosis in Human Models of Immunity to Tuberculosis. Infect. Immun. 73: 3547-3558 [Abstract] [Full Text]  
  • Weldingh, K., Rosenkrands, I., Okkels, L. M., Doherty, T. M., Andersen, P. (2005). Assessing the Serodiagnostic Potential of 35 Mycobacterium tuberculosis Proteins and Identification of Four Novel Serological Antigens. J. Clin. Microbiol. 43: 57-65 [Abstract] [Full Text]  
  • Hovav, A.-H., Fishman, Y., Bercovier, H. (2005). Gamma Interferon and Monophosphoryl Lipid A-Trehalose Dicorynomycolate Are Efficient Adjuvants for Mycobacterium tuberculosis Multivalent Acellular Vaccine. Infect. Immun. 73: 250-257 [Abstract] [Full Text]  
  • Tjalsma, H., Antelmann, H., Jongbloed, J. D.H., Braun, P. G., Darmon, E., Dorenbos, R., Dubois, J.-Y. F., Westers, H., Zanen, G., Quax, W. J., Kuipers, O. P., Bron, S., Hecker, M., van Dijl, J. M. (2004). Proteomics of Protein Secretion by Bacillus subtilis: Separating the "Secrets" of the Secretome. Microbiol. Mol. Biol. Rev. 68: 207-233 [Abstract] [Full Text]  
  • Huard, R. C., Chitale, S., Leung, M., Lazzarini, L. C. O., Zhu, H., Shashkina, E., Laal, S., Conde, M. B., Kritski, A. L., Belisle, J. T., Kreiswirth, B. N., Lapa e Silva, J. R., Ho, J. L. (2003). The Mycobacterium tuberculosis Complex-Restricted Gene cfp32 Encodes an Expressed Protein That Is Detectable in Tuberculosis Patients and Is Positively Correlated with Pulmonary Interleukin-10. Infect. Immun. 71: 6871-6883 [Abstract] [Full Text]  
  • Camus, J.-C., Pryor, M. J., Medigue, C., Cole, S. T. (2002). Re-annotation of the genome sequence of Mycobacterium tuberculosis H37Rv. Microbiology 148: 2967-2973 [Abstract] [Full Text]  
  • Cole, S.T. (2002). Comparative mycobacterial genomics as a tool for drug target and antigen discovery. Eur Respir J 20: 78S-86s [Abstract] [Full Text]  
  • Recchi, C., Rauzier, J., Gicquel, B., Reyrat, J.-M. (2002). Signal-sequence-independent secretion of the staphylococcal nuclease in Mycobacterium smegmatis. Microbiology 148: 529-536 [Abstract] [Full Text]  
  • Florczyk, M. A., McCue, L. A., Stack, R. F., Hauer, C. R., McDonough, K. A. (2001). Identification and Characterization of Mycobacterial Proteins Differentially Expressed under Standing and Shaking Culture Conditions, Including Rv2623 from a Novel Class of Putative ATP-Binding Proteins. Infect. Immun. 69: 5777-5785 [Abstract] [Full Text]  
  • Olsen, I., Tryland, M., Wiker, H. G., Reitan, L. J. (2001). AhpC, AhpD, and a Secreted 14-Kilodalton Antigen from Mycobacterium avium subsp. paratuberculosis Distinguish between Paratuberculosis and Bovine Tuberculosis in an Enzyme-Linked Immunosorbent Assay. CVI 8: 797-801 [Abstract] [Full Text]  
  • Dahl, J. L., Wei, J., Moulder, J. W., Laal, S., Friedman, R. L. (2001). Subcellular Localization of the Intracellular Survival-Enhancing Eis Protein of Mycobacterium tuberculosis. Infect. Immun. 69: 4295-4302 [Abstract] [Full Text]  
  • Olsen, I., Reitan, L. J., Wiker, H. G. (2000). Distinct Differences in Repertoires of Low-Molecular-Mass Secreted Antigens of Mycobacterium avium Complex and Mycobacterium tuberculosis. J. Clin. Microbiol. 38: 4453-4458 [Abstract] [Full Text]  
  • Betts, J. C., Dodson, P., Quan, S., Lewis, A. P., Thomas, P. J., Duncan, K., McAdam, R. A. (2000). Comparison of the proteome of Mycobacterium tuberculosis strain H37Rv with clinical isolate CDC 1551. Microbiology 146: 3205-3216 [Abstract] [Full Text]  
  • Wiker, H. G., Wilson, M. A., Schoolnik, G. K. (2000). Extracytoplasmic proteins of Mycobacterium tuberculosis - mature secreted proteins often start with aspartic acid and proline. Microbiology 146: 1525-1533 [Abstract] [Full Text]  
  • Musser, J. M., Amin, A., Ramaswamy, S. (2000). Negligible Genetic Diversity of Mycobacterium tuberculosis Host Immune System Protein Targets: Evidence of Limited Selective Pressure. Genetics 155: 7-16 [Abstract] [Full Text]  
  • Gomez, M., Johnson, S., Gennaro, M. L. (2000). Identification of Secreted Proteins of Mycobacterium tuberculosis by a Bioinformatic Approach. Infect. Immun. 68: 2323-2327 [Abstract] [Full Text]  
  • Skjot, R. L. V., Oettinger, T., Rosenkrands, I., Ravn, P., Brock, I., Jacobsen, S., Andersen, P. (2000). Comparative Evaluation of Low-Molecular-Mass Proteins from Mycobacterium tuberculosis Identifies Members of the ESAT-6 Family as Immunodominant T-Cell Antigens. Infect. Immun. 68: 214-220 [Abstract] [Full Text]  
  • Manabe, Y. C., Chen, J. M., Ko, C. G., Chen, P., Bishai, W. R. (1999). Conditional Sigma Factor Expression, Using the Inducible Acetamidase Promoter, Reveals that the Mycobacterium tuberculosis sigF Gene Modulates Expression of the 16-Kilodalton Alpha-Crystallin Homologue. J. Bacteriol. 181: 7629-7633 [Abstract] [Full Text]  
  • Rosenkrands, I., Weldingh, K., Ravn, P., Brandt, L., Hojrup, P., Rasmussen, P. B., Coates, A. R., Singh, M., Mascagni, P., Andersen, P. (1999). Differential T-Cell Recognition of Native and Recombinant Mycobacterium tuberculosis GroES. Infect. Immun. 67: 5552-5558 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»