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Infect Immun, August 1998, p. 3643-3648, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

An Epitope Delivery System for Use with Recombinant Mycobacteria

Charlotte Hetzel,1 Riny Janssen,2 Sarah J. Ely,2 Nanna M. Kristensen,2 Karen Bunting,2 3 Jonathan B. Cooper,3 Jonathan R. Lamb,1 dagger Douglas B. Young,2 and Jelle E. R. Thole2 *

Department of Biology1 and Department of Infectious Diseases and Microbiology,2 Imperial College School of Medicine, London, and Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton,3 United Kingdom

Received 29 December 1997/Returned for modification 5 March 1998/Accepted 12 May 1998

We have developed a novel epitope delivery system based on the insertion of peptides within a permissive loop of a bacterial superoxide dismutase molecule. This system allowed high-level expression of heterologous peptides in two mycobacterial vaccine strains, Mycobacterium bovis bacille Calmette-Guérin (BCG) and Mycobacterium vaccae. The broader application of the system was analyzed by preparation of constructs containing peptide epitopes from a range of infectious agents and allergens. We report detailed characterization of the immunogenicity of one such construct, in which an epitope from the Der p1 house dust mite allergen was expressed in M. vaccae. The construct was able to stimulate T-cell hybridomas specific for Der p1, and it induced peptide-specific gamma interferon responses when used to immunize naive mice. This novel expression system demonstrates new possibilities for the use of mycobacteria as vaccine delivery vehicles.

* Corresponding author. Present address: TNO Prevention and Health, Zernikedreef 9, P.O. Box 2215, 2301 CE Leiden, The Netherlands. Phone: 3171-5181276. Fax: 3171-5181901. E-mail: j.thole{at}pg.tno.nl.

dagger Present address: Respiratory Medicine Unit, University of Edinburgh Medical School, Edinburgh, United Kingdom.

Infect Immun, August 1998, p. 3643-3648, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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