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Infect Immun, August 1998, p. 3649-3655, Vol. 66, No. 8
Department of Microbiology, National
University of Ireland, Galway, Ireland,1 and
Department of Medical Microbiology, Red Cross Children's
Hospital, Rondebosch, Cape Town, South Africa2
Received 14 November 1997/Returned for modification 6 January
1998/Accepted 26 May 1998
Three Campylobacter jejuni, biotype 2, serotype O:41
strains that were isolated from patients who developed
Guillain-Barré syndrome (GBS) and one C. jejuni
isolate from a patient who developed enteritis only were examined. The
aim of the study was to determine the structure of the core
oligosaccharide (OS) of the lipopolysaccharide (LPS) of
C. jejuni serotype O:41, a serotype rarely associated with
the development of GBS, and to determine if the LPS shares similar
epitopes with any of the major human gangliosides. Electrophoretic analysis with silver staining or immunoblotting demonstrated that the
strains had LPS profiles characteristic of low-molecular-weight LPS.
Colorimetric analysis detected N-acetylneuraminic (sialic) acid in the core OSs of all the strains. Thin-layer chromatography with
immunostaining showed that antisera raised against the GBS strains
reacted with the GM1 ganglioside, suggesting that C. jejuni serotype O:41 LPSs and the GM1
ganglioside have similar epitopes. Furthermore, polyclonal
anti-GM1 and anti-asialoGM1 antibodies cross-reacted with each C. jejuni O:41 LPS tested,
suggesting that the serotype O:41 core OS has a GM1- and
asialoGM1-like structure. LPSs extracted from C. jejuni serostrains O:2, O:3, and O:19 were also used in the
study. Cholera toxin (a GM1 ligand) and peanut agglutinin
(a Gal
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Lipopolysaccharides from Campylobacter jejuni O:41
Strains Associated with Guillain-Barré Syndrome Exhibit Mimicry
of GM1 Ganglioside
1-3GalNAc ligand) recognized all serotype O:41 LPSs and the
serostrain O:2 LPS. Immunoadsorption results confirmed GM1
relatedness. Moreover, the core OS was isolated from a GBS-associated C. jejuni O:41 LPS by gel permeation chromatography. An
analysis by gas-liquid chromatography (GLC), GLC-mass
spectrometry, and nuclear magnetic resonance showed the core OS of one
of the C. jejuni O:41 GBS isolates to have a
tetrasaccharide structure consistent with GM1 mimicry.
*
Corresponding author. Mailing address: Department of
Microbiology, National University of Ireland, Galway, Ireland. Phone: 353-91-524411. Fax: 353-91-525700. E-mail:
anthony.moran{at}ucg.ie.
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