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Infect Immun, August 1998, p. 3964-3967, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Constitutive and Inducible Green Fluorescent Protein Expression in Bartonella henselae

Anthea K. Lee* and Stanley Falkow

Department of Microbiology and Immunology, Stanford University, Stanford, California 94305

Received 18 March 1998/Returned for modification 23 April 1998/Accepted 12 May 1998

The green fluorescent protein (GFP) gene was expressed on a plasmid in B. henselae, and GFP-expressing bacteria were visualized by fluorescence microscopy. HEp-2 cells infected with GFP-expressing bacteria were separated from uninfected cells with a fluorescence activated cell sorter. Promoter fusions of B. henselae chromosomal DNA to gfp were examined by flow cytometry, and a B. henselae groEL promoter fusion which induced expression at 37°C was isolated.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, 299 Campus Dr. D037, Stanford, CA 94305-5124. Phone: (650) 723-2671. Fax: (650) 723-1837. E-mail: aklee{at}leland.stanford.edu.

Infect Immun, August 1998, p. 3964-3967, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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