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Infect Immun, August 1998, p. 3978-3980, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of BrkA Expression in Bordetella bronchiseptica

Amy A. Rambow, Rachel C. Fernandez,dagger and Alison A. Weiss*

Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, Cincinnati, Ohio 45267

Received 24 November 1997/Returned for modification 8 January 1998/Accepted 29 April 1998

BrkA confers resistance to killing by complement in Bordetella pertussis. Complement resistance in Bordetella bronchiseptica was examined. Four B. bronchiseptica strains possessed the brkA gene; however, only three expressed the protein. Only the strain lacking BrkA was susceptible to complement. Introduction of the B. pertussis brkA gene restored BrkA expression to this strain but did not confer resistance. brkA was mutated in the strains that naturally expressed BrkA, and loss of BrkA did not confer sensitivity to complement. As a species, B. bronchiseptica is more resistant to complement than B. pertussis, and BrkA does not mediate resistance.

* Corresponding author. Mailing address: Molecular Genetics, Biochemistry and Microbiology, 231 Bethesda Ave., ML 524, University of Cincinnati, Cincinnati, OH 45267. Phone: (513) 558-2820. Fax: (513) 558-8474. E-mail: Alison.Weiss{at}UC.edu or rachelf{at}unixg.ubc.ca.

dagger Present address: Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3.

Infect Immun, August 1998, p. 3978-3980, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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