This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kimsey, H. H.
Right arrow Articles by Waldor, M. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kimsey, H. H.
Right arrow Articles by Waldor, M. K.

 Previous Article  |  Next Article 

Infection and Immunity, September 1998, p. 4025-4029, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Vibrio cholerae Hemagglutinin/Protease Inactivates CTXphi

Harvey H. Kimsey, and Matthew K. Waldor*

Division of Geographic Medicine, Tupper Research Institute, New England Medical Center, Boston, Massachusetts 02111

Received 4 March 1998/Returned for modification 13 May 1998/Accepted 2 June 1998

Pathogenic strains of Vibrio cholerae are lysogens of the filamentous phage CTXphi , which carries the genes for cholera toxin (ctxAB). We found that the titers of infective CTXphi in culture supernatants of El Tor CTXphi lysogens increased rapidly during exponential growth but dropped to undetectable levels late in stationary-phase growth. When CTXphi transducing particles were mixed with stationary-phase culture supernatants of El Tor strains, CTXphi infectivity was destroyed. Our data indicate that this growth phase-regulated factor, designated CDF (CTXphi -destroying factor), is the secreted hemagglutinin/protease (HA/P) of V. cholerae. A strain containing a disrupted hap gene, which encodes HA/P of V. cholerae, did not produce CDF activity in culture supernatants. Introduction of the HA/P-expressing plasmid pCH2 restored CDF activity. Also, CDF activity in culture supernatants of a variety of pathogenic V. cholerae isolates varied widely but correlated with the levels of secreted HA/P, as measured by immunoblotting with anti-HA/P antibody. CDF was purified from V. cholerae culture supernatants and shown to contain a 45-kDa polypeptide which bound anti-HA/P antibodies and which comigrated with HA/P in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The production of high levels of secreted HA/P by certain V. cholerae strains may be a factor in preventing CTXphi reinfection in natural environments and in the human host.

* Corresponding author. Mailing address: NEMC #041, 750 Washington St., Boston, MA 02111. Phone: (617) 636-7618. Fax: (617) 636-5292. E-mail: matthew.waldor{at}es.nemc.org.

Infection and Immunity, September 1998, p. 4025-4029, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ghosh, A., Saha, D. R., Hoque, K. M., Asakuna, M., Yamasaki, S., Koley, H., Das, S. S., Chakrabarti, M. K., Pal, A. (2006). Enterotoxigenicity of Mature 45-Kilodalton and Processed 35-Kilodalton Forms of Hemagglutinin Protease Purified from a Cholera Toxin Gene-Negative Vibrio cholerae Non-O1, Non-O139 Strain.. Infect. Immun. 74: 2937-2946 [Abstract] [Full Text]  
  • Halpern, M., Gancz, H., Broza, M., Kashi, Y. (2003). Vibrio cholerae Hemagglutinin/Protease Degrades Chironomid Egg Masses. Appl. Environ. Microbiol. 69: 4200-4204 [Abstract] [Full Text]  
  • Faruque, S. M., Zhu, J., Asadulghani, , Kamruzzaman, M., Mekalanos, J. J. (2003). Examination of Diverse Toxin-Coregulated Pilus-Positive Vibrio cholerae Strains Fails To Demonstrate Evidence for Vibrio Pathogenicity Island Phage. Infect. Immun. 71: 2993-2999 [Abstract] [Full Text]  
  • Berkes, J, Viswanathan, V K, Savkovic, S D, Hecht, G (2003). Intestinal epithelial responses to enteric pathogens: effects on the tight junction barrier, ion transport, and inflammation. Gut 52: 439-451 [Abstract] [Full Text]  
  • Benitez, J. A., Silva, A. J., Finkelstein, R. A. (2001). Environmental Signals Controlling Production of Hemagglutinin/Protease in Vibrio cholerae. Infect. Immun. 69: 6549-6553 [Abstract] [Full Text]  
  • Valle, E., Ledon, T., Cedre, B., Campos, J., Valmaseda, T., Rodriguez, B., Garcia, L., Marrero, K., Benitez, J., Rodriguez, S., Fando, R. (2000). Construction and Characterization of a Nonproliferative El Tor Cholera Vaccine Candidate Derived from Strain 638. Infect. Immun. 68: 6411-6418 [Abstract] [Full Text]  
  • Mel, S. F., Fullner, K. J., Wimer-Mackin, S., Lencer, W. I., Mekalanos, J. J. (2000). Association of Protease Activity in Vibrio cholerae Vaccine Strains with Decreases in Transcellular Epithelial Resistance of Polarized T84 Intestinal Epithelial Cells. Infect. Immun. 68: 6487-6492 [Abstract] [Full Text]  
  • Faruque, S. M., Asadulghani, , Rahman, M. M., Waldor, M. K., Sack, D. A. (2000). Sunlight-Induced Propagation of the Lysogenic Phage Encoding Cholera Toxin. Infect. Immun. 68: 4795-4801 [Abstract] [Full Text]  
  • Davis, B. M., Waldor, M. K. (2000). CTXphi contains a hybrid genome derived from tandemly integrated elements. Proc. Natl. Acad. Sci. USA 10.1073/pnas.140109997v1 [Abstract] [Full Text]  
  • Davis, B. M., Kimsey, H. H., Chang, W., Waldor, M. K. (1999). The Vibrio cholerae O139 Calcutta Bacteriophage CTXphi Is Infectious and Encodes a Novel Repressor. J. Bacteriol. 181: 6779-6787 [Abstract] [Full Text]  
  • Davis, B. M., Waldor, M. K. (2000). CTXphi contains a hybrid genome derived from tandemly integrated elements. Proc. Natl. Acad. Sci. USA 97: 8572-8577 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»