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Infection and Immunity, September 1998, p. 4043-4049, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Contributions of Reactive Oxygen Intermediates and Reactive Nitrogen Intermediates to Listericidal Mechanisms Differ in Macrophages Activated Pre- and Postinfection

Satoshi Ohya,1,2,* Yoshinari Tanabe,1,2 Masato Makino,1,2 Takamasa Nomura,3 Huabao Xiong,1 Masaaki Arakawa,2 and Masao Mitsuyama3

Departments of Bacteriology1 and Internal Medicine (II),2 Niigata University School of Medicine, Niigata 951-8510, and Department of Microbiology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501,3 Japan

Received 29 December 1997/Returned for modification 23 March 1998/Accepted 3 June 1998

The contribution of reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI) to the killing of Listeria monocytogenes by macrophages activated by addition of spleen cells from listeria-immune mice plus specific antigen was examined. When macrophages were infected with L. monocytogenes and then spleen cells were added, there was not as big a difference in listericidal activity between macrophages cultured with normal spleen cells and those cultured with immune spleen cells as expected. In this culture system, RNI was mainly involved in the macrophage intracellular killing. In macrophages first activated and then infected, a significant level of enhanced killing was observed. Blockade of ROI production drastically affected the enhanced killing ability, while inhibition of RNI production had a negligible effect. Thus, the contributions of ROI and RNI to listericidal mechanisms of macrophages were different between macrophages activated at pre- and postinfection stages.

* Corresponding author. Mailing address: Department of Bacteriology, Niigata University School of Medicine, 1-757, Asahimachi-dori, Niigata 951-8510, Japan. Phone: (81)25-227-2110. Fax: (81)25-227-0762. E-mail: sohya{at}med.niigata-u.ac.jp.

Infection and Immunity, September 1998, p. 4043-4049, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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