This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zubery, Y.
Right arrow Articles by Boyce, B. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zubery, Y.
Right arrow Articles by Boyce, B. F.

 Previous Article  |  Next Article 

Infection and Immunity, September 1998, p. 4158-4162, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Bone Resorption Caused by Three Periodontal Pathogens In Vivo in Mice Is Mediated in Part by Prostaglandin

Yuval Zubery,1 Colin R. Dunstan,2 Beryl M. Story,2 Lakshmyya Kesavalu,1 Jeffrey L. Ebersole,1,* Stanley C. Holt,3 and Brendan F. Boyce2

Departments of Periodontics,1 Pathology,2 and Microbiology,3 University of Texas Health Science Center at San Antonio, San Antonio, Texas 78284-7894

Received 1 December 1997/Returned for modification 2 February 1998/Accepted 10 June 1998

Gingival inflammation, bacterial infection, alveolar bone destruction, and subsequent tooth loss are characteristic features of periodontal disease, but the precise mechanisms of bone loss are poorly understood. Most animal models of the disease require injury to gingival tissues or teeth, and the effects of microorganisms are thus complicated by host responses to tissue destruction. To determine whether three putative periodontal pathogens, Porphyromonas gingivalis, Campylobacter rectus, and Fusobacterium nucleatum, could cause localized bone resorption in vivo in the absence of tissue injury, we injected live or heat-killed preparations of these microorganisms into the subcutaneous tissues overlying the calvaria of normal mice once daily for 6 days and then examined the bones histologically. We found that all three microorganisms (both live and heat killed) stimulated bone resorption and that the strain of F. nucleatum used appeared to be the strongest inducer of osteoclast activity. Treatment of the mice concomitantly with indomethacin reduced but did not completely inhibit bone resorption by these microorganisms, suggesting that their effects were mediated, in part, by arachidonic acid metabolites (e.g., prostaglandins). Our findings indicate that these potential pathogens can stimulate bone resorption locally when placed beside a bone surface in vivo in the absence of prior tissue injury and support a role for them in the pathogenesis of bone loss around teeth in periodontitis.

* Corresponding author. Mailing address: Department of Periodontics, School of Dentistry, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78284-7894. Phone: (210) 567-3591. Fax: (210) 567-6858. E-mail: EBERSOLE{at}UTHSCSA.EDU.

Infection and Immunity, September 1998, p. 4158-4162, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kuula, H., Salo, T., Pirila, E., Tuomainen, A. M., Jauhiainen, M., Uitto, V.-J., Tjaderhane, L., Pussinen, P. J., Sorsa, T. (2009). Local and Systemic Responses in Matrix Metalloproteinase 8-Deficient Mice during Porphyromonas gingivalis-Induced Periodontitis. Infect. Immun. 77: 850-859 [Abstract] [Full Text]  
  • Behl, Y., Siqueira, M., Ortiz, J., Li, J., Desta, T., Faibish, D., Graves, D. T. (2008). Activation of the Acquired Immune Response Reduces Coupled Bone Formation in Response to a Periodontal Pathogen. J. Immunol. 181: 8711-8718 [Abstract] [Full Text]  
  • Kesavalu, L., Sathishkumar, S., Bakthavatchalu, V., Matthews, C., Dawson, D., Steffen, M., Ebersole, J. L. (2007). Rat Model of Polymicrobial Infection, Immunity, and Alveolar Bone Resorption in Periodontal Disease. Infect. Immun. 75: 1704-1712 [Abstract] [Full Text]  
  • Leone, C. W., Bokhadhoor, H., Kuo, D., Desta, T., Yang, J., Siqueira, M. F., Amar, S., Graves, D. T. (2006). Immunization Enhances Inflammation and Tissue Destruction in Response to Porphyromonas gingivalis. Infect. Immun. 74: 2286-2292 [Abstract] [Full Text]  
  • Graves, D.T., Naguib, G., Lu, H., Leone, C., Hsue, H., Krall, E. (2005). Inflammation is More Persistent in Type 1 Diabetic Mice. JDR 84: 324-328 [Abstract] [Full Text]  
  • Graves, D. T., Naguib, G., Huafei Lu, , Desta, T., Amar, S. (2005). Porphyromonas gingivalis fimbriae are pro-inflammatory but do not play a prominent role in the innate immune response to P. gingivalis. Innate Immunity 11: 13-18 [Abstract]  
  • Liu, R., Desta, T., He, H., Graves, D. T. (2004). Diabetes Alters the Response to Bacteria by Enhancing Fibroblast Apoptosis. Endocrinology 145: 2997-3003 [Abstract] [Full Text]  
  • Choi, B.-K., Lee, H. J., Kang, J. H., Jeong, G. J., Min, C. K., Yoo, Y.-J. (2003). Induction of Osteoclastogenesis and Matrix Metalloproteinase Expression by the Lipooligosaccharide of Treponema denticola. Infect. Immun. 71: 226-233 [Abstract] [Full Text]  
  • Jiang, Y., Mehta, C. K., Hsu, T.-Y., Alsulaimani, F. F. H. (2002). Bacteria Induce Osteoclastogenesis via an Osteoblast-Independent Pathway. Infect. Immun. 70: 3143-3148 [Abstract] [Full Text]  
  • Graves, D.T., Oskoui, M., Voleinikova, S., Naguib, G., Cai, S., Desta, T., Kakouras, A., Jiang, Y. (2001). Tumor Necrosis Factor Modulates Fibroblast Apoptosis, PMN Recruitment, and Osteoclast Formation in Response to P. gingivalis Infection. JDR 80: 1875-1879 [Abstract]  
  • Sakuma, Y., Tanaka, K., Suda, M., Komatsu, Y., Yasoda, A., Miura, M., Ozasa, A., Narumiya, S., Sugimoto, Y., Ichikawa, A., Ushikubi, F., Nakao, K. (2000). Impaired Bone Resorption by Lipopolysaccharide In Vivo in Mice Deficient in the Prostaglandin E Receptor EP4 Subtype. Infect. Immun. 68: 6819-6825 [Abstract] [Full Text]  
  • Chiang, C.-Y., Kyritsis, G., Graves, D. T., Amar, S. (1999). Interleukin-1 and Tumor Necrosis Factor Activities Partially Account for Calvarial Bone Resorption Induced by Local Injection of Lipopolysaccharide. Infect. Immun. 67: 4231-4236 [Abstract] [Full Text]  
  • Jiang, Y., Magli, L., Russo, M. (1999). Bacterium-Dependent Induction of Cytokines in Mononuclear Cells and Their Pathologic Consequences In Vivo. Infect. Immun. 67: 2125-2130 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»