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Infection and Immunity, September 1998, p. 4163-4168, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Specificity Patterns of Human Immunoglobulin G Antibodies in Serum Differ from Those in Autologous Secretions

Armand Berneman,1 Laurent Belec,2 Vincent A. Fischetti,3 and Jean-Pierre Bouvet1,*

Unité d'Immunocytochimie, Institut Pasteur, 75724 Paris 15,1 and Service de Microbiologie, Hôpital Broussais, 75674 Paris 14,2 France, and Laboratory of Bacterial Pathogenesis, Rockefeller University, New York, New York 100213

Received 16 March 1998/Returned for modification 1 May 1998/Accepted 8 June 1998

The specificity patterns of immunoglobulin G (IgG) antibodies to streptococcal antigens in serum and autologous secretions were compared in order to determine whether IgG found in human secretions is exclusively of serum origin or can also be locally produced irrespective of the systemic immune system. Surface antigens from a type 6 M-protein strain of Streptococcus pyogenes were extracted by cell wall digestion and subjected to sodium lauryl sulfate-polyacrylamide gel electrophoresis under reducing conditions. After being blotted onto nitrocellulose, the antigens were incubated with purified IgG from various body fluids: saliva, cervicovaginal secretions, seminal fluid, and colostrum. Binding was then revealed with labeled antibodies to human Fcgamma fragments. The antibody specificity patterns obtained by computer-assisted analysis were compared with those of paired sera. Major variations were observed between serum and secretions, as well as between different secretions from the same subject. These results are in favor of IgG-associated local immunity within different tissue compartments. This IgG response to mucosal antigens can complement that of secretory IgA in the defense against pathogens and should be taken into account during topical vaccinations.

* Corresponding author. Mailing address: Unité INSERM U430, Hôpital Broussais, 96 rue Didot, 75674 Paris 14, France. Phone: (33) 1 43 95 95 83. Fax: (33) 1 45 45 90 59. E-mail: jean-pierre.bouvet{at}brs.ap-hop-paris.fr.

Infection and Immunity, September 1998, p. 4163-4168, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.