This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ASM journals
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, R.
Right arrow Articles by Weiss, W. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, R.
Right arrow Articles by Weiss, W. R.

 Previous Article  |  Next Article 

Infection and Immunity, September 1998, p. 4193-4202, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Simultaneous Induction of Multiple Antigen-Specific Cytotoxic T Lymphocytes in Nonhuman Primates by Immunization with a Mixture of Four Plasmodium falciparum DNA Plasmids

Ruobing Wang,1,2 Denise L. Doolan,1,dagger Yupin Charoenvit,1 Richard C. Hedstrom,1 Malcolm J. Gardner,1,Dagger Peter Hobart,3 John Tine,4 Martha Sedegah,1,5 Victoria Fallarme,1,2 John B. Sacci Jr.,1,5 Manjit Kaur,1 Dennis M. Klinman,6 Stephen L. Hoffman,1 and Walter R. Weiss1,*

Malaria Program, Naval Medical Research Institute, Bethesda, Maryland 208891; Henry M. Jackson Foundation, Rockville, Maryland 208522; Vical Incorporated, San Diego, California 921213; Virogenetics Corporation, Troy, New York 121804; Department of Microbiology and Immunology, University of Maryland, Baltimore, Maryland 212015; and Section of Retroviral Immunology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 208926

Received 9 February 1998/Returned for modification 10 April 1998/Accepted 4 June 1998

CD8+ T cells have been implicated as critical effector cells in protective immunity against malaria parasites developing within hepatocytes. A vaccine that protects against malaria by inducing CD8+ T cells will probably have to include multiple epitopes on the same protein or different proteins, because of parasite polymorphism and genetic restriction of T-cell responses. To determine if CD8+ T-cell responses against multiple P. falciparum proteins can be induced in primates by immunization with plasmid DNA, rhesus monkeys were immunized intramuscularly with a mixture of DNA plasmids encoding four P. falciparum proteins or with individual plasmids. All six monkeys immunized with PfCSP DNA, seven of nine immunized with PfSSP2 DNA, and five of six immunized with PfExp-1 or PfLSA-1 DNA had detectable antigen-specific cytotoxic T lymphocytes (CTL) after in vitro restimulation of peripheral blood mononuclear cells. CTL activity was genetically restricted and dependent on CD8+ T cells. By providing the first evidence for primates that immunization with a mixture of DNA plasmids induces CD8+ T-cell responses against all the components of the mixture, these studies provide the foundation for multigene immunization of humans.

* Corresponding author. Mailing address: Malaria Program, Naval Medical Research Institute, 12300 Washington Ave., Rockville, MD 20852. Phone: (301) 295-1705. Fax: (301) 295-6171. E-mail: weissw{at}nmripo.nmri.nnmc.navy.mil.

dagger Present address: Queensland Institute Medical Research, Queensland, Australia.

Dagger Present address: The Institute for Genomic Research, Rockville, MD.

Infection and Immunity, September 1998, p. 4193-4202, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Wang, R., Richie, T. L., Baraceros, M. F., Rahardjo, N., Gay, T., Banania, J.-G., Charoenvit, Y., Epstein, J. E., Luke, T., Freilich, D. A., Norman, J., Hoffman, S. L. (2005). Boosting of DNA Vaccine-Elicited Gamma Interferon Responses in Humans by Exposure to Malaria Parasites. Infect. Immun. 73: 2863-2872 [Abstract] [Full Text]  
  • Rainczuk, A., Scorza, T., Spithill, T. W., Smooker, P. M. (2004). A Bicistronic DNA Vaccine Containing Apical Membrane Antigen 1 and Merozoite Surface Protein 4/5 Can Prime Humoral and Cellular Immune Responses and Partially Protect Mice against Virulent Plasmodium chabaudi adami DS Malaria. Infect. Immun. 72: 5565-5573 [Abstract] [Full Text]  
  • BALLOU, W. R., AREVALO-HERRERA, M., CARUCCI, D., RICHIE, T. L., CORRADIN, G., DIGGS, C., DRUILHE, P., GIERSING, B. K., SAUL, A., HEPPNER, D. G., KESTER, K. E., LANAR, D. E., LYON, J., HILL, A. V. S., PAN, W., COHEN, J. D. (2004). UPDATE ON THE CLINICAL DEVELOPMENT OF CANDIDATE MALARIA VACCINES. Am J Trop Med Hyg 71: 239-247 [Abstract] [Full Text]  
  • Coban, C., Philipp, M. T., Purcell, J. E., Keister, D. B., Okulate, M., Martin, D. S., Kumar, N. (2004). Induction of Plasmodium falciparum Transmission-Blocking Antibodies in Nonhuman Primates by a Combination of DNA and Protein Immunizations. Infect. Immun. 72: 253-259 [Abstract] [Full Text]  
  • O'Hagan, D., Singh, M., Ugozzoli, M., Wild, C., Barnett, S., Chen, M., Schaefer, M., Doe, B., Otten, G. R., Ulmer, J. B. (2001). Induction of Potent Immune Responses by Cationic Microparticles with Adsorbed Human Immunodeficiency Virus DNA Vaccines. J. Virol. 75: 9037-9043 [Abstract] [Full Text]  
  • Rogers, W. O., Baird, J. K., Kumar, A., Tine, J. A., Weiss, W., Aguiar, J. C., Gowda, K., Gwadz, R., Kumar, S., Gold, M., Hoffman, S. L. (2001). Multistage Multiantigen Heterologous Prime Boost Vaccine for Plasmodium knowlesi Malaria Provides Partial Protection in Rhesus Macaques. Infect. Immun. 69: 5565-5572 [Abstract] [Full Text]  
  • Wang, R., Epstein, J., Baraceros, F. M., Gorak, E. J., Charoenvit, Y., Carucci, D. J., Hedstrom, R. C., Rahardjo, N., Gay, T., Hobart, P., Stout, R., Jones, T. R., Richie, T. L., Parker, S. E., Doolan, D. L., Norman, J., Hoffman, S. L. (2001). Induction of CD4+ T cell-dependent CD8+ type 1 responses in humans by a malaria DNA vaccine. Proc. Natl. Acad. Sci. USA 10.1073/pnas.181123498v1 [Abstract] [Full Text]  
  • Wang, R., Doolan, D. L., Le, T. P., Hedstrom, R. C., Coonan, K. M., Charoenvit, Y., Jones, T. R., Hobart, P., Margalith, M., Ng, J., Weiss, W. R., Sedegah, M., de Taisne, C., A. Norman, J., Hoffman, S. L. (1998). Induction of Antigen-Specific Cytotoxic T Lymphocytes in Humans by a Malaria DNA Vaccine. Science 282: 476-480 [Abstract] [Full Text]  
  • Wang, R., Epstein, J., Baraceros, F. M., Gorak, E. J., Charoenvit, Y., Carucci, D. J., Hedstrom, R. C., Rahardjo, N., Gay, T., Hobart, P., Stout, R., Jones, T. R., Richie, T. L., Parker, S. E., Doolan, D. L., Norman, J., Hoffman, S. L. (2001). Induction of CD4+ T cell-dependent CD8+ type 1 responses in humans by a malaria DNA vaccine. Proc. Natl. Acad. Sci. USA 98: 10817-10822 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»