This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jain-Vora, S.
Right arrow Articles by Whitsett, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jain-Vora, S.
Right arrow Articles by Whitsett, J. A.

 Previous Article  |  Next Article 

Infection and Immunity, September 1998, p. 4229-4236, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Interleukin-4 Enhances Pulmonary Clearance of Pseudomonas aeruginosa

Shilpa Jain-Vora, Ann Marie LeVine, Zissis Chroneos, Gary F. Ross, William M. Hull, and Jeffrey A. Whitsett*

Division of Pulmonary Biology, Children's Hospital Medical Center, Cincinnati, Ohio 45229-3039

Received 14 November 1997/Returned for modification 4 March 1998/Accepted 17 June 1998

To determine the effects of interleukin-4 (IL-4) on bacterial clearance from the mouse lung, transgenic mice expressing IL-4 in respiratory epithelial cells under the control of the Clara cell secretory protein promoter (CCSP-IL-4 mice) were infected intratracheally with Pseudomonas aeruginosa. Survival of CCSP-IL-4 mice following bacterial administration was markedly improved compared with that of control mice. While bacteria proliferated in lungs of wild-type mice, a rapid reduction in the number of bacteria was observed in the IL-4 mice as early as 6 h postinfection. Similarly, intranasal administration of IL-4 enhanced bacterial clearance from the lungs of wild-type mice. While acute and chronic IL-4 increased the numbers of neutrophils in bronchoalveolar lavage fluid, bacterial infection was associated with acute neutrophilic pulmonary infiltration, and this response was similar in the presence or absence of IL-4. Local administration or expression of IL-4 in the mouse lung enhanced pulmonary clearance of P. aeruginosa in vivo and decreased mortality following infection.

* Corresponding author. Mailing address: Children's Hospital Medical Center, Divisions of Neonatology and Pulmonary Biology, 3333 Burnet Ave., Cincinnati, OH 45229-3039. Phone: (513) 636-4830. Fax: (513) 636-7868. E-mail: JEFF.WHITSETT{at}CHMCC.ORG.

Infection and Immunity, September 1998, p. 4229-4236, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nguyen, M., Pace, A. J., Koller, B. H. (2007). Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia. JEM 204: 1383-1393 [Abstract] [Full Text]  
  • Giannoni, E., Sawa, T., Allen, L., Wiener-Kronish, J., Hawgood, S. (2006). Surfactant Proteins A and D Enhance Pulmonary Clearance of Pseudomonas aeruginosa. Am. J. Respir. Cell Mol. Bio. 34: 704-710 [Abstract] [Full Text]  
  • Haczku, A., Cao, Y., Vass, G., Kierstein, S., Nath, P., Atochina-Vasserman, E. N., Scanlon, S. T., Li, L., Griswold, D. E., Chung, K. F., Poulain, F. R., Hawgood, S., Beers, M. F., Crouch, E. C. (2006). IL-4 and IL-13 Form a Negative Feedback Circuit with Surfactant Protein-D in the Allergic Airway Response. J. Immunol. 176: 3557-3565 [Abstract] [Full Text]  
  • Sadikot, R. T., Blackwell, T. S., Christman, J. W., Prince, A. S. (2005). Pathogen-Host Interactions in Pseudomonas aeruginosa Pneumonia. Am. J. Respir. Crit. Care Med. 171: 1209-1223 [Abstract] [Full Text]  
  • Dave, V., Childs, T., Whitsett, J. A. (2004). Nuclear Factor of Activated T Cells Regulates Transcription of the Surfactant Protein D Gene (Sftpd) via Direct Interaction with Thyroid Transcription Factor-1 in Lung Epithelial Cells. J. Biol. Chem. 279: 34578-34588 [Abstract] [Full Text]  
  • Song, Z., Wu, H., Ciofu, O., Kong, K.-F., Hoiby, N., Rygaard, J., Kharazmi, A., Mathee, K. (2003). Pseudomonas aeruginosa alginate is refractory to Th1 immune response and impedes host immune clearance in a mouse model of acute lung infection. J Med Microbiol 52: 731-740 [Abstract] [Full Text]  
  • Gildea, L. A., Gibbons, R., Finkelman, F. D., Deepe, G. S. Jr. (2003). Overexpression of Interleukin-4 in Lungs of Mice Impairs Elimination of Histoplasma capsulatum. Infect. Immun. 71: 3787-3793 [Abstract] [Full Text]  
  • He, Y., Crouch, E. (2002). Surfactant Protein D Gene Regulation. INTERACTIONS AMONG THE CONSERVED CCAAT/ENHANCER-BINDING PROTEIN ELEMENTS. J. Biol. Chem. 277: 19530-19537 [Abstract] [Full Text]  
  • Worgall, S., Kikuchi, T., Singh, R., Martushova, K., Lande, L., Crystal, R. G. (2001). Protection against Pulmonary Infection with Pseudomonas aeruginosa following Immunization with P. aeruginosa-Pulsed Dendritic Cells. Infect. Immun. 69: 4521-4527 [Abstract] [Full Text]  
  • Grohmann, U., Van Snick, J., Campanile, F., Silla, S., Giampietri, A., Vacca, C., Renauld, J.-C., Fioretti, M. C., Puccetti, P. (2000). IL-9 Protects Mice from Gram-Negative Bacterial Shock: Suppression of TNF-{alpha}, IL-12, and IFN-{gamma}, and Induction of IL-10. J. Immunol. 164: 4197-4203 [Abstract] [Full Text]  
  • Opal, S. M., DePalo, V. A. (2000). Anti-Inflammatory Cytokines. Chest 117: 1162-1172 [Abstract] [Full Text]  
  • Ikegami, M., Whitsett, J. A., Chroneos, Z. C., Ross, G. F., Reed, J. A., Bachurski, C. J., Jobe, A. H. (2000). IL-4 increases surfactant and regulates metabolism in vivo. Am. J. Physiol. Lung Cell. Mol. Physiol. 278: L75-L80 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»