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Infection and Immunity, September 1998, p. 4313-4323, Vol. 66, No. 9
Department of Biochemistry and Molecular
Biology,1
Department of Microbiology and
Molecular Genetics,2
Division of
Infectious Diseases,
Received 21 April 1998/Returned for modification 28 May
1998/Accepted 17 June 1998
Our previous work identified a cosmid clone containing a 43-kb
insert from Enterococcus faecalis OG1RF that produced a
nonprotein antigen in Escherichia coli. In the present
work, we studied this clone in detail. Periodate treatment of lysates
of the clone confirmed that the antigen was carbohydrate in nature.
Analysis of DNA sequences and transposon insertion mutants suggested
that the insert contained a multicistronic gene cluster. Database
comparison showed that the cluster contained genes similar to genes
involved in the biosynthesis of dTDP-rhamnose, glycosyltransferases,
and ABC transporters involved in the export of sugar polymers from both
gram-positive and gram-negative organisms. Insertions in several genes
within the cluster abolished the immunoreactivity of the clone. This is
the first report on a gene cluster of E. faecalis
involved in the biosynthesis of an antigenic polysaccharide.
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
A Cluster of Genes Involved in Polysaccharide
Biosynthesis from Enterococcus faecalis OG1RF
*
Corresponding author. Mailing address: Department of
Microbiology and Molecular Genetics, University of Texas Medical
School, 6431 Fannin St., Houston, TX 77030. Phone: (713) 500-6083. Fax: (713) 500-5499. E-mail:
georgew{at}utmmg.med.uth.tmc.edu.
Infection and Immunity, September 1998, p. 4313-4323, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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