This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Xu, Y.
Right arrow Articles by Weinstock, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xu, Y.
Right arrow Articles by Weinstock, G. M.

 Previous Article  |  Next Article 

Infection and Immunity, September 1998, p. 4313-4323, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Cluster of Genes Involved in Polysaccharide Biosynthesis from Enterococcus faecalis OG1RF

Yi Xu,1 Barbara E. Murray,2,3,4 and George M. Weinstock1,2,3,4,*

Department of Biochemistry and Molecular Biology,1 Department of Microbiology and Molecular Genetics,2 Division of Infectious Diseases, Department of Medicine,3 and Center for the Study of Emerging and Re-Emerging Pathogens,4 University of Texas Medical School, Houston, Texas 77030

Received 21 April 1998/Returned for modification 28 May 1998/Accepted 17 June 1998

Our previous work identified a cosmid clone containing a 43-kb insert from Enterococcus faecalis OG1RF that produced a nonprotein antigen in Escherichia coli. In the present work, we studied this clone in detail. Periodate treatment of lysates of the clone confirmed that the antigen was carbohydrate in nature. Analysis of DNA sequences and transposon insertion mutants suggested that the insert contained a multicistronic gene cluster. Database comparison showed that the cluster contained genes similar to genes involved in the biosynthesis of dTDP-rhamnose, glycosyltransferases, and ABC transporters involved in the export of sugar polymers from both gram-positive and gram-negative organisms. Insertions in several genes within the cluster abolished the immunoreactivity of the clone. This is the first report on a gene cluster of E. faecalis involved in the biosynthesis of an antigenic polysaccharide.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, University of Texas Medical School, 6431 Fannin St., Houston, TX 77030. Phone: (713) 500-6083. Fax: (713) 500-5499. E-mail: georgew{at}utmmg.med.uth.tmc.edu.

Infection and Immunity, September 1998, p. 4313-4323, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Thurlow, L. R., Thomas, V. C., Hancock, L. E. (2009). Capsular Polysaccharide Production in Enterococcus faecalis and Contribution of CpsF to Capsule Serospecificity. J. Bacteriol. 191: 6203-6210 [Abstract] [Full Text]  
  • Teng, F., Singh, K. V., Bourgogne, A., Zeng, J., Murray, B. E. (2009). Further Characterization of the epa Gene Cluster and Epa Polysaccharides of Enterococcus faecalis. Infect. Immun. 77: 3759-3767 [Abstract] [Full Text]  
  • Yasuda, E., Serata, M., Sako, T. (2008). Suppressive Effect on Activation of Macrophages by Lactobacillus casei Strain Shirota Genes Determining the Synthesis of Cell Wall-Associated Polysaccharides. Appl. Environ. Microbiol. 74: 4746-4755 [Abstract] [Full Text]  
  • Lepage, E., Brinster, S., Caron, C., Ducroix-Crepy, C., Rigottier-Gois, L., Dunny, G., Hennequet-Antier, C., Serror, P. (2006). Comparative Genomic Hybridization Analysis of Enterococcus faecalis: Identification of Genes Absent from Food Strains.. J. Bacteriol. 188: 6858-6868 [Abstract] [Full Text]  
  • Hufnagel, M., Kropec, A., Theilacker, C., Huebner, J. (2005). Naturally Acquired Antibodies against Four Enterococcus faecalis Capsular Polysaccharides in Healthy Human Sera. CVI 12: 930-934 [Abstract] [Full Text]  
  • Mohamed, J. A., Huang, W., Nallapareddy, S. R., Teng, F., Murray, B. E. (2004). Influence of Origin of Isolates, Especially Endocarditis Isolates, and Various Genes on Biofilm Formation by Enterococcus faecalis. Infect. Immun. 72: 3658-3663 [Abstract] [Full Text]  
  • Hufnagel, M., Hancock, L. E., Koch, S., Theilacker, C., Gilmore, M. S., Huebner, J. (2004). Serological and Genetic Diversity of Capsular Polysaccharides in Enterococcus faecalis. J. Clin. Microbiol. 42: 2548-2557 [Abstract] [Full Text]  
  • Novotny, R., Schaffer, C., Strauss, J., Messner, P. (2004). S-layer glycan-specific loci on the chromosome of Geobacillus stearothermophilus NRS 2004/3a and dTDP-L-rhamnose biosynthesis potential of G. stearothermophilus strains. Microbiology 150: 953-965 [Abstract] [Full Text]  
  • Zeng, J., Teng, F., Weinstock, G. M., Murray, B. E. (2004). Translocation of Enterococcus faecalis Strains across a Monolayer of Polarized Human Enterocyte-Like T84 Cells. J. Clin. Microbiol. 42: 1149-1154 [Abstract] [Full Text]  
  • Teng, F., Kawalec, M., Weinstock, G. M., Hryniewicz, W., Murray, B. E. (2003). An Enterococcus faecium Secreted Antigen, SagA, Exhibits Broad-Spectrum Binding to Extracellular Matrix Proteins and Appears Essential for E. faecium Growth. Infect. Immun. 71: 5033-5041 [Abstract] [Full Text]  
  • Hancock, L. E., Shepard, B. D., Gilmore, M. S. (2003). Molecular Analysis of the Enterococcus faecalis Serotype 2 Polysaccharide Determinant. J. Bacteriol. 185: 4393-4401 [Abstract] [Full Text]  
  • Priya, C. G., Bhavani, K., Rathinam, S. R., Muthukkaruppan, V. R. (2003). Identification and evaluation of LPS antigen for serodiagnosis of uveitis associated with leptospirosis. J Med Microbiol 52: 667-673 [Abstract] [Full Text]  
  • Bao, Q., Tian, Y., Li, W., Xu, Z., Xuan, Z., Hu, S., Dong, W., Yang, J., Chen, Y., Xue, Y., Xu, Y., Lai, X., Huang, L., Dong, X., Ma, Y., Ling, L., Tan, H., Chen, R., Wang, J., Yu, J., Yang, H. (2002). A Complete Sequence of the T. tengcongensis Genome. Genome Res 12: 689-700 [Abstract] [Full Text]  
  • Teng, F., Jacques-Palaz, K. D., Weinstock, G. M., Murray, B. E. (2002). Evidence that the Enterococcal Polysaccharide Antigen Gene (epa) Cluster Is Widespread in Enterococcus faecalis and Influences Resistance to Phagocytic Killing of E. faecalis. Infect. Immun. 70: 2010-2015 [Abstract] [Full Text]  
  • Smajs, D., McKevitt, M., Wang, L., Howell, J. K., Norris, S. J., Palzkill, T., Weinstock, G. M. (2002). BAC Library of T. pallidum DNA in E. coli. Genome Res 12: 515-522 [Abstract] [Full Text]  
  • Hancock, L. E., Gilmore, M. S. (2002). The capsular polysaccharide of Enterococcus faecalis and its relationship to other polysaccharides in the cell wall. Proc. Natl. Acad. Sci. USA 99: 1574-1579 [Abstract] [Full Text]  
  • Arora, S. K., Bangera, M., Lory, S., Ramphal, R. (2001). A genomic island in Pseudomonas aeruginosa carries the determinants of flagellin glycosylation. Proc. Natl. Acad. Sci. USA 98: 9342-9347 [Abstract] [Full Text]  
  • Fujibuchi, W., Ogata, H., Matsuda, H., Kanehisa, M. (2000). Automatic detection of conserved gene clusters in multiple genomes by graph comparison and P-quasi grouping. Nucleic Acids Res 28: 4029-4036 [Abstract] [Full Text]  
  • Nallapareddy, S. R., Singh, K. V., Duh, R.-W., Weinstock, G. M., Murray, B. E. (2000). Diversity of ace, a Gene Encoding a Microbial Surface Component Recognizing Adhesive Matrix Molecules, from Different Strains of Enterococcus faecalis and Evidence for Production of Ace during Human Infections. Infect. Immun. 68: 5210-5217 [Abstract] [Full Text]  
  • Huebner, J., Quaas, A., Krueger, W. A., Goldmann, D. A., Pier, G. B. (2000). Prophylactic and Therapeutic Efficacy of Antibodies to a Capsular Polysaccharide Shared among Vancomycin-Sensitive and -Resistant Enterococci. Infect. Immun. 68: 4631-4636 [Abstract] [Full Text]  
  • Kihara, D., Kanehisa, M. (2000). Tandem Clusters of Membrane Proteins in Complete Genome Sequences. Genome Res 10: 731-743 [Abstract] [Full Text]  
  • Xu, Y., Singh, K. V., Qin, X., Murray, B. E., Weinstock, G. M. (2000). Analysis of a Gene Cluster of Enterococcus faecalis Involved in Polysaccharide Biosynthesis. Infect. Immun. 68: 815-823 [Abstract] [Full Text]  
  • Yamashita, Y., Shibata, Y., Nakano, Y., Tsuda, H., Kido, N., Ohta, M., Koga, T. (1999). A Novel Gene Required for Rhamnose-Glucose Polysaccharide Synthesis in Streptococcus mutans. J. Bacteriol. 181: 6556-6559 [Abstract] [Full Text]  
  • Graninger, M., Nidetzky, B., Heinrichs, D. E., Whitfield, C., Messner, P. (1999). Characterization of dTDP-4-dehydrorhamnose 3,5-Epimerase and dTDP-4-dehydrorhamnose Reductase, Required for dTDP-L-rhamnose Biosynthesis in Salmonella enterica Serovar Typhimurium LT2. J. Biol. Chem. 274: 25069-25077 [Abstract] [Full Text]  
  • Huebner, J., Wang, Y., Krueger, W. A., Madoff, L. C., Martirosian, G., Boisot, S., Goldmann, D. A., Kasper, D. L., Tzianabos, A. O., Pier, G. B. (1999). Isolation and Chemical Characterization of a Capsular Polysaccharide Antigen Shared by Clinical Isolates of Enterococcus faecalis and Vancomycin-Resistant Enterococcus faecium. Infect. Immun. 67: 1213-1219 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»