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Infection and Immunity, September 1998, p. 4313-4323, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Cluster of Genes Involved in Polysaccharide Biosynthesis from Enterococcus faecalis OG1RF

Yi Xu,1 Barbara E. Murray,2,3,4 and George M. Weinstock1,2,3,4,*

Department of Biochemistry and Molecular Biology,1 Department of Microbiology and Molecular Genetics,2 Division of Infectious Diseases, Department of Medicine,3 and Center for the Study of Emerging and Re-Emerging Pathogens,4 University of Texas Medical School, Houston, Texas 77030

Received 21 April 1998/Returned for modification 28 May 1998/Accepted 17 June 1998

Our previous work identified a cosmid clone containing a 43-kb insert from Enterococcus faecalis OG1RF that produced a nonprotein antigen in Escherichia coli. In the present work, we studied this clone in detail. Periodate treatment of lysates of the clone confirmed that the antigen was carbohydrate in nature. Analysis of DNA sequences and transposon insertion mutants suggested that the insert contained a multicistronic gene cluster. Database comparison showed that the cluster contained genes similar to genes involved in the biosynthesis of dTDP-rhamnose, glycosyltransferases, and ABC transporters involved in the export of sugar polymers from both gram-positive and gram-negative organisms. Insertions in several genes within the cluster abolished the immunoreactivity of the clone. This is the first report on a gene cluster of E. faecalis involved in the biosynthesis of an antigenic polysaccharide.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, University of Texas Medical School, 6431 Fannin St., Houston, TX 77030. Phone: (713) 500-6083. Fax: (713) 500-5499. E-mail: georgew{at}utmmg.med.uth.tmc.edu.

Infection and Immunity, September 1998, p. 4313-4323, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.