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Infection and Immunity, September 1998, p. 4440-4449, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

CD86 (B7-2), but Not CD80 (B7-1), Expression in the Epidermis of Transgenic Mice Enhances the Immunogenicity of Primary Cutaneous Candida albicans Infections

Anthony A. Gaspari,1,2,3,* Robert Burns,1 Adnan Nasir,1,dagger Diana Ramirez,4,Dagger Richard K. Barth,2,4 and Constantine G. Haidaris3,4

Department of Dermatology,1 Cancer Center,2 Strong Children's Research Center,3 and Department of Microbiology and Immunology,4 University of Rochester School of Medicine and Dentistry, Rochester, New York 14642

Received 3 March 1998/Returned for modification 7 May 1998/Accepted 24 June 1998

Transgenic (Tg) mice whose epidermal keratinocytes constitutively overexpress either B7-1 (CD80) or B7-2 (CD86) exhibited exaggerated cutaneous delayed type hypersensitivity (DTH) to haptens compared to non-Tg mice. To determine whether enhanced DTH in these Tg mice is seen in response to cutaneous fungal infections, a primary infection with Candida albicans was established by inoculating this organism on the occluded skin of Tg and non-Tg mice. These infections resolved 7 days after removal of occlusive dressing in all three groups of mice, without evidence of exaggerated inflammation in either the Tg or non-Tg mice. Only B7-2 Tg mice developed enhanced Th1-lymphocyte-mediated immune responses to C. albicans antigens after resolving this infection: enhanced footpad swelling in response to intradermal C. albicans antigens, enhanced production of mRNA encoding Th1 lymphokines in draining lymph nodes, and increased gamma interferon secreted into culture supernatants by lymph node T lymphocytes stimulated with Candida antigens in vitro. Lastly, Western blotting of sera from mice that had resolved this fungal infection indicated that only B7-2 Tg mice recognized a wide range of Candida-associated antigens. These data suggest that these two costimulatory molecules, when expressed by keratinocytes, do not deliver identical signals to C. albicans antigen-reactive Th1 lymphocytes. The enhanced immune response in B7-2 Tg mice to a cutaneous C. albicans infection demonstrates the importance of antigen presentation and costimulation in immune reactivity to fungi. Furthermore, B7-2 Tg mice may be useful in identification of protective Candida antigens.

* Corresponding author. Mailing address: 601 Elmwood Ave., Box 697, Rochester, NY 14642. Phone: (716) 275-1802. Fax: (716) 271-4715. E-mail: Anthony_Gaspari{at}urmc.rochester.edu.

dagger Present address: Department of Dermatology, University of North Carolina---Chapel Hill, Chapel Hill, NC.

Dagger Present address: Department of Hematology, Columbia University, New York, NY.

Infection and Immunity, September 1998, p. 4440-4449, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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