This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Acheson, D. W. K.
Right arrow Articles by Waldor, M. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Acheson, D. W. K.
Right arrow Articles by Waldor, M. K.

 Previous Article  |  Next Article 

Infection and Immunity, September 1998, p. 4496-4498, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

In Vivo Transduction with Shiga Toxin 1-Encoding Phage

David W. K. Acheson,1 Joachim Reidl,2 Xiaoping Zhang,1 Gerald T. Keusch,1 John J. Mekalanos,3 and Matthew K. Waldor1,*

Division of Geographic Medicine and Infectious Diseases and Tupper Research Institute, Tufts University School of Medicine,1 and Department of Microbiology and Molecular Genetics and the Shipley Institute of Medicine, Harvard Medical School,3 Boston, Massachusetts, and Research Center for Infectious Disease, University of Wuerzburg, Wuerzburg, Germany2

Received 3 April 1998/Returned for modification 15 May 1998/Accepted 2 June 1998

To facilitate the study of intestinal transmission of the Shiga toxin 1 (Stx1)-converting phage H-19B, Tn10d-bla mutagenesis of an Escherichia coli H-19B lysogen was undertaken. Two mutants containing insertions in the gene encoding the A subunit of Stx1 were isolated. The resultant ampicillin-resistant E. coli strains lysogenic for these phages produced infectious H-19B particles but not active toxin. These lysogens were capable of transducing an E. coli recipient strain in the murine gastrointestinal tract, thereby demonstrating that lysogens of Shiga toxin-converting phages give rise to infectious virions within the host gastrointestinal tract.

* Corresponding author. Mailing address: Division of Geographic Medicine and Infectious Diseases, NEMC 041, 750 Washington St., Boston, MA 02111. Phone: (617) 636-7618. Fax: (617) 636-5292. E-mail: matthew.waldor{at}es.nemc.org.

Infection and Immunity, September 1998, p. 4496-4498, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Toth, I., Schmidt, H., Kardos, G., Lancz, Z., Creuzburg, K., Damjanova, I., Paszti, J., Beutin, L., Nagy, B. (2009). Virulence Genes and Molecular Typing of Different Groups of Escherichia coli O157 Strains in Cattle. Appl. Environ. Microbiol. 75: 6282-6291 [Abstract] [Full Text]  
  • Imamovic, L., Jofre, J., Schmidt, H., Serra-Moreno, R., Muniesa, M. (2009). Phage-Mediated Shiga Toxin 2 Gene Transfer in Food and Water. Appl. Environ. Microbiol. 75: 1764-1768 [Abstract] [Full Text]  
  • Garcia-Aljaro, C., Muniesa, M., Jofre, J., Blanch, A. R. (2009). Genotypic and Phenotypic Diversity among Induced, stx2-Carrying Bacteriophages from Environmental Escherichia coli Strains. Appl. Environ. Microbiol. 75: 329-336 [Abstract] [Full Text]  
  • Toth, I., Nougayrede, J.-P., Dobrindt, U., Ledger, T. N., Boury, M., Morabito, S., Fujiwara, T., Sugai, M., Hacker, J., Oswald, E. (2009). Cytolethal Distending Toxin Type I and Type IV Genes Are Framed with Lambdoid Prophage Genes in Extraintestinal Pathogenic Escherichia coli. Infect. Immun. 77: 492-500 [Abstract] [Full Text]  
  • Serra-Moreno, R., Jofre, J., Muniesa, M. (2008). The CI Repressors of Shiga Toxin-Converting Prophages Are Involved in Coinfection of Escherichia coli Strains, Which Causes a Down Regulation in the Production of Shiga Toxin 2. J. Bacteriol. 190: 4722-4735 [Abstract] [Full Text]  
  • Mellmann, A., Lu, S., Karch, H., Xu, J.-g., Harmsen, D., Schmidt, M. A., Bielaszewska, M. (2008). Recycling of Shiga Toxin 2 Genes in Sorbitol-Fermenting Enterohemorrhagic Escherichia coli O157:NM. Appl. Environ. Microbiol. 74: 67-72 [Abstract] [Full Text]  
  • Serra-Moreno, R., Jofre, J., Muniesa, M. (2007). Insertion Site Occupancy by stx2 Bacteriophages Depends on the Locus Availability of the Host Strain Chromosome. J. Bacteriol. 189: 6645-6654 [Abstract] [Full Text]  
  • Bielaszewska, M., Prager, R., Kock, R., Mellmann, A., Zhang, W., Tschape, H., Tarr, P. I., Karch, H. (2007). Shiga Toxin Gene Loss and Transfer In Vitro and In Vivo during Enterohemorrhagic Escherichia coli O26 Infection in Humans. Appl. Environ. Microbiol. 73: 3144-3150 [Abstract] [Full Text]  
  • Willenbrock, H., Petersen, A., Sekse, C., Kiil, K., Wasteson, Y., Ussery, D. W. (2006). Design of a Seven-Genome Escherichia coli Microarray for Comparative Genomic Profiling. J. Bacteriol. 188: 7713-7721 [Abstract] [Full Text]  
  • Cornick, N. A., Helgerson, A. F., Mai, V., Ritchie, J. M., Acheson, D. W. K. (2006). In vivo transduction of an stx-encoding phage in ruminants.. Appl. Environ. Microbiol. 72: 5086-5088 [Abstract] [Full Text]  
  • Zhang, H., Marconi, R. T. (2005). Demonstration of Cotranscription and 1-Methyl-3-Nitroso-Nitroguanidine Induction of a 30-Gene Operon of Borrelia burgdorferi: Evidence that the 32-Kilobase Circular Plasmids Are Prophages. J. Bacteriol. 187: 7985-7995 [Abstract] [Full Text]  
  • Aertsen, A., Faster, D., Michiels, C. W. (2005). Induction of Shiga Toxin-Converting Prophage in Escherichia coli by High Hydrostatic Pressure. Appl. Environ. Microbiol. 71: 1155-1162 [Abstract] [Full Text]  
  • Creuzburg, K., Kohler, B., Hempel, H., Schreier, P., Jacobs, E., Schmidt, H. (2005). Genetic structure and chromosomal integration site of the cryptic prophage CP-1639 encoding Shiga toxin 1. Microbiology 151: 941-950 [Abstract] [Full Text]  
  • Gamage, S. D., Patton, A. K., Hanson, J. F., Weiss, A. A. (2004). Diversity and Host Range of Shiga Toxin-Encoding Phage. Infect. Immun. 72: 7131-7139 [Abstract] [Full Text]  
  • Brussow, H., Canchaya, C., Hardt, W.-D. (2004). Phages and the Evolution of Bacterial Pathogens: from Genomic Rearrangements to Lysogenic Conversion. Microbiol. Mol. Biol. Rev. 68: 560-602 [Abstract] [Full Text]  
  • Muniesa, M., Blanco, J. E., de Simon, M., Serra-Moreno, R., Blanch, A. R., Jofre, J. (2004). Diversity of stx2 converting bacteriophages induced from Shiga-toxin-producing Escherichia coli strains isolated from cattle. Microbiology 150: 2959-2971 [Abstract] [Full Text]  
  • Garcia-Aljaro, C., Muniesa, M., Jofre, J., Blanch, A. R. (2004). Prevalence of the stx2 Gene in Coliform Populations from Aquatic Environments. Appl. Environ. Microbiol. 70: 3535-3540 [Abstract] [Full Text]  
  • Breitbart, M., Wegley, L., Leeds, S., Schoenfeld, T., Rohwer, F. (2004). Phage Community Dynamics in Hot Springs. Appl. Environ. Microbiol. 70: 1633-1640 [Abstract] [Full Text]  
  • Faruque, S. M., Chowdhury, N., Khan, R., Hasan, M. R., Nahar, J., Islam, M. J., Yamasaki, S., Ghosh, A. N., Nair, G. B., Sack, D. A. (2003). Shigella dysenteriae Type 1-Specific Bacteriophage from Environmental Waters in Bangladesh. Appl. Environ. Microbiol. 69: 7028-7031 [Abstract] [Full Text]  
  • Toth, I., Schmidt, H., Dow, M., Malik, A., Oswald, E., Nagy, B. (2003). Transduction of Porcine Enteropathogenic Escherichia coli with a Derivative of a Shiga Toxin 2-Encoding Bacteriophage in a Porcine Ligated Ileal Loop System. Appl. Environ. Microbiol. 69: 7242-7247 [Abstract] [Full Text]  
  • Muniesa, M., de Simon, M., Prats, G., Ferrer, D., Panella, H., Jofre, J. (2003). Shiga Toxin 2-Converting Bacteriophages Associated with Clonal Variability in Escherichia coli O157:H7 Strains of Human Origin Isolated from a Single Outbreak. Infect. Immun. 71: 4554-4562 [Abstract] [Full Text]  
  • Broudy, T. B., Fischetti, V. A. (2003). In Vivo Lysogenic Conversion of Tox- Streptococcus pyogenes to Tox+ with Lysogenic Streptococci or Free Phage. Infect. Immun. 71: 3782-3786 [Abstract] [Full Text]  
  • Gamage, S. D., Strasser, J. E., Chalk, C. L., Weiss, A. A. (2003). Nonpathogenic Escherichia coli Can Contribute to the Production of Shiga Toxin. Infect. Immun. 71: 3107-3115 [Abstract] [Full Text]  
  • Wagner, P. L., Waldor, M. K. (2002). Bacteriophage Control of Bacterial Virulence. Infect. Immun. 70: 3985-3993 [Full Text]  
  • Sandt, C. H., Hopper, J. E., Hill, C. W. (2002). Activation of Prophage eib Genes for Immunoglobulin-Binding Proteins by Genes from the IbrAB Genetic Island of Escherichia coli ECOR-9. J. Bacteriol. 184: 3640-3648 [Abstract] [Full Text]  
  • Venkatesan, M. M., Hartman, A. B., Newland, J. W., Ivanova, V. S., Hale, T. L., McDonough, M., Butterton, J. (2002). Construction, Characterization, and Animal Testing of WRSd1, a Shigella dysenteriae 1 Vaccine. Infect. Immun. 70: 2950-2958 [Abstract] [Full Text]  
  • Strauch, E., Lurz, R., Beutin, L. (2001). Characterization of a Shiga Toxin-Encoding Temperate Bacteriophage of Shigella sonnei. Infect. Immun. 69: 7588-7595 [Abstract] [Full Text]  
  • Barkocy-Gallagher, G. A., Arthur, T. M., Siragusa, G. R., Keen, J. E., Elder, R. O., Laegreid, W. W., Koohmaraie, M. (2001). Genotypic Analyses of Escherichia coli O157:H7 and O157 Nonmotile Isolates Recovered from Beef Cattle and Carcasses at Processing Plants in the Midwestern States of the United States. Appl. Environ. Microbiol. 67: 3810-3818 [Abstract] [Full Text]  
  • James, C. E., Stanley, K. N., Allison, H. E., Flint, H. J., Stewart, C. S., Sharp, R. J., Saunders, J. R., McCarthy, A. J. (2001). Lytic and Lysogenic Infection of Diverse Escherichia coli and Shigella Strains with a Verocytotoxigenic Bacteriophage. Appl. Environ. Microbiol. 67: 4335-4337 [Abstract] [Full Text]  
  • Elliott, E J, Robins-Browne, R M, O'Loughlin, E V, Bennett-Wood, V, Bourke, J, Henning, P, Hogg, G G, Knight, J, Powell, H, Redmond, D, Contributors to the Australian Paediatric Surveill, (2001). Nationwide study of haemolytic uraemic syndrome: clinical, microbiological, and epidemiological features. Arch. Dis. Child. 85: 125-131 [Abstract] [Full Text]  
  • Broudy, T. B., Pancholi, V., Fischetti, V. A. (2001). Induction of Lysogenic Bacteriophage and Phage-Associated Toxin from Group A Streptococci during Coculture with Human Pharyngeal Cells. Infect. Immun. 69: 1440-1443 [Abstract] [Full Text]  
  • OSAWA, R., IYODA, S., NAKAYAMA, S.-I., WADA, A., YAMAI, S., WATANABE, H. (2000). Genotypic variations of Shiga toxin-converting phages from enterohaemorrhagic Escherichia coli O157: H7 isolates. J Med Microbiol 49: 565-574 [Abstract] [Full Text]  
  • Muniesa, M., Lucena, F., Jofre, J. (1999). Comparative Survival of Free Shiga Toxin 2-Encoding Phages and Escherichia coli Strains outside the Gut. Appl. Environ. Microbiol. 65: 5615-5618 [Abstract] [Full Text]  
  • Wagner, P. L., Acheson, D. W. K., Waldor, M. K. (1999). Isogenic Lysogens of Diverse Shiga Toxin 2-Encoding Bacteriophages Produce Markedly Different Amounts of Shiga Toxin. Infect. Immun. 67: 6710-6714 [Abstract] [Full Text]  
  • Schmidt, H., Bielaszewska, M., Karch, H. (1999). Transduction of Enteric Escherichia coli Isolates with a Derivative of Shiga Toxin 2-Encoding Bacteriophage phi 3538 Isolated from Escherichia coli O157:H7. Appl. Environ. Microbiol. 65: 3855-3861 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»