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Infection and Immunity, September 1998, p. 4511-4516, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Binding of Heme-Hemopexin Complexes by Soluble HxuA Protein Allows Utilization of This Complexed Heme by Haemophilus influenzae

Leslie D. Cope,1 Sharon E. Thomas,1 Zbynek Hrkal,2 and Eric J. Hansen1,*

Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9048,1 and Department of Cellular Biochemistry, Institute of Haemotology and Blood Transfusion, 128 20 Prague-2, Czech Republic2

Received 27 February 1998/Returned for modification 2 April 1998/Accepted 17 June 1998

Utilization of heme-hemopexin as a source of heme by Haemophilus influenzae type b is dependent on expression by this bacterium of the 100-kDa HxuA protein, which is both present on the bacterial cell surface and released into the culture supernatant (L. D. Cope, R. Yogev, U. Muller-Eberhard, and E. J. Hansen, J. Bacteriol. 177:2644-2653, 1995). Radioimmunoprecipitation analysis showed that the soluble HxuA protein present in H. influenzae type b culture supernatant bound heme-hemopexin complexes in solution. An isogenic H. influenzae type b hxuA mutant was unable to utilize soluble heme-hemopexin complexes for growth in vitro unless soluble HxuA protein was provided exogenously. Soluble HxuA protein secreted by a nontypeable H. influenzae strain also allowed growth of this H. influenzae type b hxuA mutant. These results indicated that the heme present in heme-hemopexin complexes is rendered accessible to H. influenzae when these complexes are bound by the soluble HxuA protein.

* Corresponding author. Mailing address: Department of Microbiology, University of Texas Southwestern Medical Center, Hamon Biomedical Research Building, NA6.200A, 6000 Harry Hines Blvd., Dallas, TX 75235-9048. Phone: (214) 648-5974. Fax: (214) 648-5905. E-mail: hansen01{at}utsw.swmed.edu.

Infection and Immunity, September 1998, p. 4511-4516, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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