Escherichia coli O157:H7 Requires Intimin for Enteropathogenicity in Calves

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Infection and Immunity, September 1998, p. 4560-4563, Vol. 66, No. 9
0019-9567/98/$00.00+0

Escherichia coli O157:H7 Requires Intimin for Enteropathogenicity in Calves

Evelyn A. Dean-Nystrom,¹^,^* Brad T. Bosworth,¹ Harley W. Moon,² and Alison D. O'Brien³

Enteric Diseases and Food Safety Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010¹; Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011²; and Department of Microbiology and Immunology, F. Edward Hébert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814³

Received 8 April 1998/Returned for modification 15 May 1998/Accepted 26 June 1998

Enterohemorrhagic Escherichia coli (EHEC) strains require intimin to induce attaching and effacing (A/E) lesions in newbornpiglets. Infection of newborn calves with intimin-positive orintimin-negative EHEC O157:H7 demonstrated that intimin is neededfor colonization, A/E lesions, and disease in cattle. These resultssuggest that experiments to determine if intimin-based vaccinesreduce O157:H7 levels in cattle are warranted.

^* Corresponding author. Mailing address: USDA, ARS, National Animal Disease Center, P.O. Box 70, Ames, IA 50010-0070. Phone:(515) 239-8376. Fax: (515) 239-8458. E-mail: enystrom{at}nadc.ars.usda.gov.

Infection and Immunity, September 1998, p. 4560-4563, Vol. 66, No. 9
0019-9567/98/$00.00+0

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