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Infection and Immunity, September 1998, p. 4572-4576, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Construction of a Stable Attenuated Shigella
sonnei
virG Vaccine Strain, WRSS1, and Protective
Efficacy and Immunogenicity in the Guinea Pig
Keratoconjunctivitis Model
Antoinette B.
Hartman,* and
Malabi M.
Venkatesan
Department of Enteric Infections, Walter Reed
Army Institute of Research, Washington, D.C. 20307-5100
Received 10 April 1998/Returned for modification 1 June
1998/Accepted 18 June 1998
Construction of a stable Shigella sonnei vaccine has
been complicated by the instability of the virulence phenotype caused by the spontaneous loss of the invasion plasmid. To select a suitable candidate for vaccine construction, 16 S. sonnei
strains were screened for stability of the virulence phenotype. A
stable strain, S. sonnei Mosely, was selected for
further work. p
virG2, a deletion derivative of the virG
gene in the sacB suicide vector pCVD442, was used to
generate an S. sonnei virG deletion strain, WRSS1, which was invasive in HeLa cells but negative in the Sereny test. WRSS1
was found to be both immunogenic and protective in the guinea pig
keratoconjunctivitis model.
*
Corresponding author. Mailing address: Department of
Enteric Infections, Walter Reed Army Institute of Research, Washington, DC 20307-5100. Phone: (202) 782-3792. Fax: (202) 782-3299. E-mail: dr._antoinette_hartman{at}wrsmtp-ccmail.army.mil.
Infection and Immunity, September 1998, p. 4572-4576, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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