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Infection and Immunity, September 1998, p. 4572-4576, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Construction of a Stable Attenuated Shigella sonnei Delta virG Vaccine Strain, WRSS1, and Protective Efficacy and Immunogenicity in the Guinea Pig Keratoconjunctivitis Model

Antoinette B. Hartman,* and Malabi M. Venkatesan

Department of Enteric Infections, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100

Received 10 April 1998/Returned for modification 1 June 1998/Accepted 18 June 1998

Construction of a stable Shigella sonnei vaccine has been complicated by the instability of the virulence phenotype caused by the spontaneous loss of the invasion plasmid. To select a suitable candidate for vaccine construction, 16 S. sonnei strains were screened for stability of the virulence phenotype. A stable strain, S. sonnei Mosely, was selected for further work. pDelta virG2, a deletion derivative of the virG gene in the sacB suicide vector pCVD442, was used to generate an S. sonnei virG deletion strain, WRSS1, which was invasive in HeLa cells but negative in the Sereny test. WRSS1 was found to be both immunogenic and protective in the guinea pig keratoconjunctivitis model.

* Corresponding author. Mailing address: Department of Enteric Infections, Walter Reed Army Institute of Research, Washington, DC 20307-5100. Phone: (202) 782-3792. Fax: (202) 782-3299. E-mail: dr._antoinette_hartman{at}wrsmtp-ccmail.army.mil.

Infection and Immunity, September 1998, p. 4572-4576, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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