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Infection and Immunity, January 1999, p. 108-112, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Catecholamine Oxidative Products, but Not Melanin, Are Produced by Cryptococcus neoformans during Neuropathogenesis in Mice

Lide Liu,1 Kazumasa Wakamatsu,2 Shosuke Ito,2 and Peter R. Williamson1,*

Division of Infectious Disease, University of Illinois at Chicago College of Medicine, Chicago, Illinois 60612,1 and Fujita Health University School of Health Sciences, Toyoake, Aichi 470-1192, Japan2

Received 1 June 1998/Returned for modification 23 July 1998/Accepted 20 October 1998

Melanin has been proposed as a virulence factor in Cryptococcus neoformans, but its presence has not been shown unambiguously in vivo. Validated methods used previously to show production of cryptococcal eumelanin pigment in vitro (P. R. Williamson, K. Wakamatsu, and S. Ito, J. Bacteriol. 180:1570-1572, 1998) were used to assess for production of laccase-derived products in mouse brain of the Lacc+ strains, 2E-TUC, H99 (serotype A), and ATCC 34873 (serotype D), and the Lacc- strain, 2E-TU. Pyrrole-2,3,5-tricarboxylic and pyrrole-2,3-dicarboxylic acid, specific degradation products of catecholamine derivatives such as melanin, were found in all Lacc+ strains, but not in the Lacc- strain, 2E-TU. However, the presence of melanin pigment itself could not be demonstrated in the same cells. Lack of the specific degradation products aminohydroxyphenylalanine and aminohydroxyphenylethylamine in Lacc+ strains upon hydriodic acid hydrolysis showed that pheomelanin was also not produced by the fungus in vivo. These are the first data to support the generation of catecholamine oxidation products by C. neoformans in vivo, but they do not support postenzymatic polymerization of these products to form typical eumelanin, as previously proposed.

* Corresponding author. Mailing address: Division of Infectious Diseases, Department of Medicine, Rm 888, Bldg. 910, m/c 735, University of Illinois at Chicago College of Medicine, 808 S. Wood St., Chicago, IL 60612. Phone: (312) 996-6070. Fax: (312) 996-5704. E-mail: prw{at}uic.edu.

Infection and Immunity, January 1999, p. 108-112, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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