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Infection and Immunity, January 1999, p. 165-172, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of an Intestinal Neutral Glycosphingolipid as a Phenotype-Specific Receptor for the K88ad Fimbrial Adhesin of Escherichia colidagger

Philippe A. Grange,1 Alan K. Erickson,1,* Steven B. Levery,2 and David H. Francis1

Department of Veterinary Science, South Dakota State University, Brookings, South Dakota 57007,1 and Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 306022

Received 18 June 1998/Returned for modification 7 August 1998/Accepted 23 October 1998

In this study, we identified a receptor for the K88ad fimbrial adhesin of Escherichia coli in neutral glycosphingolipid preparations from intestinal epithelial cells of K88ad-adhesive pigs, which was absent in preparations from K88ad-nonadhesive pigs. Neither K88ab nor K88ac adhesin variants bound to this neutral glycosphingolipid. Because this receptor is an intestinal glycosphingolipid that binds K88ad adhesin, it has been designated IGLad. Carbohydrate compositional analysis of a partially purified preparation of IGLad identified galactose, glucose, and N-acetylglucosamine in a ratio of 1.5:1.0:0.5 as the major monosaccharides. Preliminary characterization experiments using lectins showed that IGLad contains the terminal glycanic structure Galbeta 1-4GlcNAc. Removal of terminal beta -linked galactose residues from IGLad decreased the recognition of IGLad by the K88ad adhesin, indicating that terminal beta -linked galactose is an essential component of the K88ad adhesin recognition site on IGLad. Studies with purified glycosphingolipid standards demonstrated that K88ad adhesin binds to neolactotetraosylceramide (nLc4Cer) (Galbeta 1-4GlcNAcbeta 1-3Galbeta 1-4Glcbeta 1-1Cer), lactotriosylceramide (GlcNAcbeta 1-3Galbeta 1-4Glcbeta 1-1Cer) and lactotetraosylceramide (Galbeta 1-3GlcNAcbeta 1-3Galbeta 1-4Glcbeta 1-1Cer). Based on these studies, IGLad appears to be nLc4Cer.

* Corresponding author. Mailing address: Department of Veterinary Science, P.O. Box 2175, South Dakota State University, Brookings, SD 57007-1396. Phone: (605) 688-5171. Fax: (605) 688-6003. E-mail: ericksoa{at}mg.sdstate.edu.

dagger South Dakota Agricultural Experiment Station paper no. 3083.

Infection and Immunity, January 1999, p. 165-172, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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