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Infection and Immunity, January 1999, p. 286-293, Vol. 67, No. 1
Divisions of Clinical Bio-Regulatory
Science,1
Gastroenterology,2 and
Anatomy
and Neurobiology,
Received 15 June 1998/Returned for modification 31 July
1998/Accepted 5 October 1998
Infection by Helicobacter pylori, a noninvasive
bacterium, induces chronic leukocyte infiltration in the stomach by
still largely unknown molecular mechanisms. We investigated the
possibility that a membrane protein of H. pylori induces an
inflammatory reaction in the subepithelial tissue of the stomach. By
generating an expression library of H. pylori chromosomal
DNA and screening with rabbit antiserum raised to a membrane fraction
of H. pylori and sera of infected patients, we cloned a
16.0-kDa protein (HP-MP1) which appeared to attach to the inner
membrane of the H. pylori in a homodimeric form.
Anti-HP-MP1 antibodies were detected in the sera of infected patients
but not in those of uninfected controls. Coincubation of monocytes with
recombinant HP-MP1 led to cell activation and production of
interleukin-1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Cloning and Characterization of a Novel
Membrane-Associated Antigenic Protein of Helicobacter
pylori
(IL-1
), tumor necrosis factor alpha, IL-8, and
macrophage inflammatory protein 1
. The results indicate that HP-MP1
is an antigenic membrane-associated protein of H. pylori
which potentially activates monocytes. This suggests that HP-MP1
may play roles in the pathogenesis of perpetual tissue inflammation
associated with H. pylori infection.
*
Corresponding author. Mailing address: Division of
Clinical Bio-Regulatory Science, Graduate School of Medicine, Kyoto
University, 54, Shogo-in, Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan. Phone: (81) 75-751-3777. Fax: (81) 75-751-4206. E-mail:
h50638{at}sakura.kudpc.kyoto-u.ac.jp.
Infection and Immunity, January 1999, p. 286-293, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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