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Infection and Immunity, January 1999, p. 286-293, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cloning and Characterization of a Novel Membrane-Associated Antigenic Protein of Helicobacter pylori

Masaru Yoshida,1,2 Yoshio Wakatsuki,1,* Yoshinao Kobayashi,3 Toshiyuki Itoh,1,2 Kazuhisa Murakami,3 Akira Mizoguchi,4 Takashi Usui,1 Tsutomu Chiba,2 and Toru Kita1

Divisions of Clinical Bio-Regulatory Science,1 Gastroenterology,2 and Anatomy and Neurobiology,4 Graduate School of Medicine, Kyoto University, Kyoto, and Shionogi Research Laboratories, Osaka,3 Japan

Received 15 June 1998/Returned for modification 31 July 1998/Accepted 5 October 1998

Infection by Helicobacter pylori, a noninvasive bacterium, induces chronic leukocyte infiltration in the stomach by still largely unknown molecular mechanisms. We investigated the possibility that a membrane protein of H. pylori induces an inflammatory reaction in the subepithelial tissue of the stomach. By generating an expression library of H. pylori chromosomal DNA and screening with rabbit antiserum raised to a membrane fraction of H. pylori and sera of infected patients, we cloned a 16.0-kDa protein (HP-MP1) which appeared to attach to the inner membrane of the H. pylori in a homodimeric form. Anti-HP-MP1 antibodies were detected in the sera of infected patients but not in those of uninfected controls. Coincubation of monocytes with recombinant HP-MP1 led to cell activation and production of interleukin-1alpha (IL-1alpha ), tumor necrosis factor alpha, IL-8, and macrophage inflammatory protein 1alpha . The results indicate that HP-MP1 is an antigenic membrane-associated protein of H. pylori which potentially activates monocytes. This suggests that HP-MP1 may play roles in the pathogenesis of perpetual tissue inflammation associated with H. pylori infection.

* Corresponding author. Mailing address: Division of Clinical Bio-Regulatory Science, Graduate School of Medicine, Kyoto University, 54, Shogo-in, Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan. Phone: (81) 75-751-3777. Fax: (81) 75-751-4206. E-mail: h50638{at}sakura.kudpc.kyoto-u.ac.jp.

Infection and Immunity, January 1999, p. 286-293, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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