This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sandlin, R. C.
Right arrow Articles by Maurelli, A. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sandlin, R. C.
Right arrow Articles by Maurelli, A. T.

 Previous Article  |  Next Article 

Infection and Immunity, January 1999, p. 350-356, Vol. 67, No. 1
0019-9567/99/$00.00+0

Establishment of Unipolar Localization of IcsA in Shigella flexneri 2a Is Not Dependent on Virulence Plasmid Determinants

Robin C. Sandlin, and Anthony T. Maurelli*

Department of Microbiology and Immunology, F. Edward Hébert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799

Received 5 June 1998/Returned for modification 23 July 1998/Accepted 6 October 1998

Unipolar localization of IcsA on the surface of Shigella flexneri is required for efficient formation of actin tails and protrusions in infected eucaryotic cells. Lipopolysaccharide (LPS) mutations have been demonstrated to affect either the establishment or the maintenance of IcsA in a unipolar location, although the mechanism is unknown. In order to analyze the contribution of virulence plasmid determinants on the unipolar localization of IcsA, we examined the localization of IcsA expressed from a cloned plasmid copy in two different genetic backgrounds. The localization of IcsA was first examined in a virulence plasmid-cured derivative of the wild-type S. flexneri 2a isolate 2457T. This approach examined the contribution of virulence plasmid-borne factors, including the previously identified virulence plasmid-borne protease that is responsible for cleaving IcsA in the outer membrane and releasing the 95-kDa secreted form from the cell surface. IcsA localization in a related but nonpathogenic Escherichia coli strain expressing LPS of the O8 serotype was also examined. IcsA surface presentation in both of these genetic backgrounds continued to be unipolar, demonstrating that virulence plasmid-borne determinants are not responsible for unipolar localization of IcsA. The unipolar localization of IcsA in the E. coli background suggests that a common pathway that allows IcsA to be spatially restricted to one pole on the bacterial cell surface exists in Shigella and E. coli.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, F. Edward Hébert School of Medicine, 4301 Jones Bridge Rd., Bethesda, MD 20814-4799. Phone: (301) 295-3415. Fax: (301) 295-1545. E-mail address: AMAURELLI{at}MXB.USUHS.MIL.

Infection and Immunity, January 1999, p. 350-356, Vol. 67, No. 1
0019-9567/99/$00.00+0


This article has been cited by other articles:

  • Janakiraman, A., Fixen, K. R., Gray, A. N., Niki, H., Goldberg, M. B. (2009). A Genome-Scale Proteomic Screen Identifies a Role for DnaK in Chaperoning of Polar Autotransporters in Shigella. J. Bacteriol. 191: 6300-6311 [Abstract] [Full Text]  
  • Clark, C. S., Maurelli, A. T. (2007). Shigella flexneri Inhibits Staurosporine-Induced Apoptosis in Epithelial Cells. Infect. Immun. 75: 2531-2539 [Abstract] [Full Text]  
  • Carballido-Lopez, R. (2006). The Bacterial Actin-Like Cytoskeleton. Microbiol. Mol. Biol. Rev. 70: 888-909 [Abstract] [Full Text]  
  • Jain, S., van Ulsen, P., Benz, I., Schmidt, M. A., Fernandez, R., Tommassen, J., Goldberg, M. B. (2006). Polar Localization of the Autotransporter Family of Large Bacterial Virulence Proteins. J. Bacteriol. 188: 4841-4850 [Abstract] [Full Text]  
  • Nilsen, T., Yan, A. W., Gale, G., Goldberg, M. B. (2005). Presence of Multiple Sites Containing Polar Material in Spherical Escherichia coli Cells That Lack MreB. J. Bacteriol. 187: 6187-6196 [Abstract] [Full Text]  
  • Wing, H. J., Yan, A. W., Goldman, S. R., Goldberg, M. B. (2004). Regulation of IcsP, the Outer Membrane Protease of the Shigella Actin Tail Assembly Protein IcsA, by Virulence Plasmid Regulators VirF and VirB. J. Bacteriol. 186: 699-705 [Abstract] [Full Text]  
  • Janakiraman, A., Goldberg, M. B. (2004). From the Cover: Evidence for polar positional information independent of cell division and nucleoid occlusion. Proc. Natl. Acad. Sci. USA 101: 835-840 [Abstract] [Full Text]  
  • Purdy, G. E., Hong, M., Payne, S. M. (2002). Shigella flexneri DegP Facilitates IcsA Surface Expression and Is Required for Efficient Intercellular Spread. Infect. Immun. 70: 6355-6364 [Abstract] [Full Text]  
  • Kane, C. D., Schuch, R., Day, W. A. Jr, Maurelli, A. T. (2002). MxiE Regulates Intracellular Expression of Factors Secreted by the Shigella flexneri 2a Type III Secretion System. J. Bacteriol. 184: 4409-4419 [Abstract] [Full Text]  
  • Mogull, S. A., Runyen-Janecky, L. J., Hong, M., Payne, S. M. (2001). dksA Is Required for Intercellular Spread of Shigella flexneri via an RpoS-Independent Mechanism. Infect. Immun. 69: 5742-5751 [Abstract] [Full Text]  
  • Charles, M., Perez, M., Kobil, J. H., Goldberg, M. B. (2001). Polar targeting of Shigella virulence factor IcsA in Enterobacteriacae and Vibrio. Proc. Natl. Acad. Sci. USA 10.1073/pnas.171310498v1 [Abstract] [Full Text]  
  • Lybarger, S. R., Maddock, J. R. (2001). Polarity in Action: Asymmetric Protein Localization in Bacteria. J. Bacteriol. 183: 3261-3267 [Full Text]  
  • Charles, M., Perez, M., Kobil, J. H., Goldberg, M. B. (2001). Polar targeting of Shigella virulence factor IcsA in Enterobacteriacae and Vibrio. Proc. Natl. Acad. Sci. USA 98: 9871-9876 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»