Previous Article | Next Article 
Infection and Immunity, January 1999, p. 350-356, Vol. 67, No. 1
0019-9567/99/$00.00+0
Establishment of Unipolar Localization of IcsA in Shigella
flexneri 2a Is Not Dependent on Virulence Plasmid
Determinants
Robin C.
Sandlin, and
Anthony T.
Maurelli*
Department of Microbiology and Immunology, F. Edward Hébert School of Medicine, Uniformed Services
University of the Health Sciences, Bethesda, Maryland 20814-4799
Received 5 June 1998/Returned for modification 23 July
1998/Accepted 6 October 1998
Unipolar localization of IcsA on the surface of Shigella
flexneri is required for efficient formation of actin tails and
protrusions in infected eucaryotic cells. Lipopolysaccharide (LPS)
mutations have been demonstrated to affect either the establishment or
the maintenance of IcsA in a unipolar location, although the mechanism is unknown. In order to analyze the contribution of virulence plasmid
determinants on the unipolar localization of IcsA, we examined the
localization of IcsA expressed from a cloned plasmid copy in two
different genetic backgrounds. The localization of IcsA was first
examined in a virulence plasmid-cured derivative of the wild-type
S. flexneri 2a isolate 2457T. This approach examined the
contribution of virulence plasmid-borne factors, including the
previously identified virulence plasmid-borne protease that is
responsible for cleaving IcsA in the outer membrane and releasing the
95-kDa secreted form from the cell surface. IcsA localization in a
related but nonpathogenic Escherichia coli strain
expressing LPS of the O8 serotype was also examined. IcsA surface
presentation in both of these genetic backgrounds continued to be
unipolar, demonstrating that virulence plasmid-borne determinants are
not responsible for unipolar localization of IcsA. The unipolar
localization of IcsA in the E. coli background suggests
that a common pathway that allows IcsA to be spatially restricted to
one pole on the bacterial cell surface exists in Shigella
and E. coli.
*
Corresponding author. Mailing address: Department of
Microbiology and Immunology, Uniformed Services University of the
Health Sciences, F. Edward Hébert School of Medicine, 4301 Jones
Bridge Rd., Bethesda, MD 20814-4799. Phone: (301) 295-3415. Fax: (301) 295-1545. E-mail address: AMAURELLI{at}MXB.USUHS.MIL.
Infection and Immunity, January 1999, p. 350-356, Vol. 67, No. 1
0019-9567/99/$00.00+0
This article has been cited by other articles:
-
Janakiraman, A., Fixen, K. R., Gray, A. N., Niki, H., Goldberg, M. B.
(2009). A Genome-Scale Proteomic Screen Identifies a Role for DnaK in Chaperoning of Polar Autotransporters in Shigella. J. Bacteriol.
191: 6300-6311
[Abstract]
[Full Text]
-
Clark, C. S., Maurelli, A. T.
(2007). Shigella flexneri Inhibits Staurosporine-Induced Apoptosis in Epithelial Cells. Infect. Immun.
75: 2531-2539
[Abstract]
[Full Text]
-
Carballido-Lopez, R.
(2006). The Bacterial Actin-Like Cytoskeleton. Microbiol. Mol. Biol. Rev.
70: 888-909
[Abstract]
[Full Text]
-
Jain, S., van Ulsen, P., Benz, I., Schmidt, M. A., Fernandez, R., Tommassen, J., Goldberg, M. B.
(2006). Polar Localization of the Autotransporter Family of Large Bacterial Virulence Proteins. J. Bacteriol.
188: 4841-4850
[Abstract]
[Full Text]
-
Nilsen, T., Yan, A. W., Gale, G., Goldberg, M. B.
(2005). Presence of Multiple Sites Containing Polar Material in Spherical Escherichia coli Cells That Lack MreB. J. Bacteriol.
187: 6187-6196
[Abstract]
[Full Text]
-
Wing, H. J., Yan, A. W., Goldman, S. R., Goldberg, M. B.
(2004). Regulation of IcsP, the Outer Membrane Protease of the Shigella Actin Tail Assembly Protein IcsA, by Virulence Plasmid Regulators VirF and VirB. J. Bacteriol.
186: 699-705
[Abstract]
[Full Text]
-
Janakiraman, A., Goldberg, M. B.
(2004). From the Cover: Evidence for polar positional information independent of cell division and nucleoid occlusion. Proc. Natl. Acad. Sci. USA
101: 835-840
[Abstract]
[Full Text]
-
Purdy, G. E., Hong, M., Payne, S. M.
(2002). Shigella flexneri DegP Facilitates IcsA Surface Expression and Is Required for Efficient Intercellular Spread. Infect. Immun.
70: 6355-6364
[Abstract]
[Full Text]
-
Kane, C. D., Schuch, R., Day, W. A. Jr, Maurelli, A. T.
(2002). MxiE Regulates Intracellular Expression of Factors Secreted by the Shigella flexneri 2a Type III Secretion System. J. Bacteriol.
184: 4409-4419
[Abstract]
[Full Text]
-
Mogull, S. A., Runyen-Janecky, L. J., Hong, M., Payne, S. M.
(2001). dksA Is Required for Intercellular Spread of Shigella flexneri via an RpoS-Independent Mechanism. Infect. Immun.
69: 5742-5751
[Abstract]
[Full Text]
-
Charles, M., Perez, M., Kobil, J. H., Goldberg, M. B.
(2001). Polar targeting of Shigella virulence factor IcsA in Enterobacteriacae and Vibrio. Proc. Natl. Acad. Sci. USA
10.1073/pnas.171310498v1
[Abstract]
[Full Text]
-
Lybarger, S. R., Maddock, J. R.
(2001). Polarity in Action: Asymmetric Protein Localization in Bacteria. J. Bacteriol.
183: 3261-3267
[Full Text]
-
Charles, M., Perez, M., Kobil, J. H., Goldberg, M. B.
(2001). Polar targeting of Shigella virulence factor IcsA in Enterobacteriacae and Vibrio. Proc. Natl. Acad. Sci. USA
98: 9871-9876
[Abstract]
[Full Text]