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Infection and Immunity, January 1999, p. 357-367, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Mucin-Related Epitopes Distinguish M Cells and Enterocytes in Rabbit Appendix and Peyer's Patches

Hugues Lelouard,1 Hubert Reggio,1 Paul Mangeat,1 Marian Neutra,2 and Philippe Montcourrier1,*

Laboratoire de Dynamique Moléculaire des Interactions Membranaires, UMR CNRS 5539, Université de Montpellier II, 34095 Montpellier Cedex 5, France,1 and Harvard Medical School and GI Cell Biology Laboratory, Children's Hospital, Boston, Massachusetts 021152

Received 4 June 1998/Returned for modification 17 August 1998/Accepted 14 September 1998

The biochemical composition of the apical membranes of epithelial M cells overlying the gut-associated lymphoid tissues (GALT) is still largely unknown. We have prepared monoclonal antibodies (MAbs) directed against carbonate-washed plasma membranes from epithelial cells detached with EDTA from rabbit appendix, a tissue particularly rich in GALT. As determined by immunofluorescence microscopy, several MAbs specifically recognized either M cells or enterocyte-like cells of the domes from rabbit appendix, sacculus rotundus, and Peyer's patches. M cells were identified by their large ventral pocket containing lymphoid cells and by specific labeling with antivimentin. Among various characterized MAbs, MAb 104 recognized rabbit immunoglobulins and was used as an apical marker for M cells in the rabbit appendix, MAb 58 selectively stained an integral membrane glycoprotein of greater than 205 kDa located at the apex of M cells, and MAb 214 stained a smaller soluble glycoprotein associated with the apical surfaces from neighboring enterocytes. In addition, both MAbs 58 and 214 also labeled luminal mucus and secretory granules in goblet cells. The selective association of mucin-related molecules at the surfaces of either M cells or enterocyte-like cells of the follicle-associated epithelium suggests that specific carbohydrate antigens are differentially expressed by epithelial cells and could account for the differential binding properties of pathogens.

* Corresponding author. Mailing address: Laboratoire de Dynamique Moléculaire des Interactions Membranaires, UMR CNRS 5539, CC 107, Université de Montpellier II, 34095 Montpellier Cedex 5, France. Phone: 33 4 67 14 47 31. Fax: 33 4 67 14 47 27. E-mail: montcour{at}univ-montp2.fr.

Infection and Immunity, January 1999, p. 357-367, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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