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Infection and Immunity, January 1999, p. 384-394, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Interleukin-6 (IL-6) and IL-8 Are Induced in Human Oral Epithelial Cells in Response to Exposure to Periodontopathic Eikenella corrodens

Hiromichi Yumoto,1,* Hideaki Nakae,1 Keiko Fujinaka,1 Shigeyuki Ebisu,2 and Takashi Matsuo1

Department of Conservative Dentistry, Tokushima University School of Dentistry, Tokushima 770,1 and Department of Conservative Dentistry, Osaka University Faculty of Dentistry, Osaka,2 Japan

Received 28 April 1998/Returned for modification 13 July 1998/Accepted 9 September 1998

Periodontitis is the inflammatory response in periodontal tissues elicited by bacterial colonization in periodontal pockets. In this response, pocket epithelial cells are the first cells to come into contact with bacteria. To elucidate this mechanism, we determined the adherence of the periodontopathic bacterium Eikenella corrodens 1073, which has a GalNAc-sensitive lectin-like adhesin (EcLS), to a human oral epithelial carcinoma cell line (KB) and the induction of proinflammatory cytokine production in the cells following exposure to this bacterium in vitro. In the adherence assay, EcLS played a role as the adhesin of this bacterium in adherence to KB cells. In a reverse transcriptase PCR, significant interleukin-8 (IL-8) and IL-6 mRNA levels were induced in response to exposure to this bacterium. In an enzyme-linked immunosorbent assay after an 8-h bacterial exposure, the IL-8 and IL-6 protein levels were 13.5- and 8.3-fold higher than those in the nonexposed controls, respectively. These protein responses were time dependent. Interestingly, when E. corrodens was separated from KB cells by cell culture inserts, a slight stimulation of the IL-6 and IL-8 mRNA and secreted protein levels was seen. These results imply that the direct contact of E. corrodens 1073 with oral epithelial cells is not necessarily required for the stimulation of IL-6 and IL-8 secretion. We suggest that E. corrodens induces the epithelial cells to secrete proinflammatory cytokines which serve as an early signaling system to host immune and inflammatory cells in underlying connective tissues.

* Corresponding author. Mailing address: Department of Conservative Dentistry, Tokushima University School of Dentistry, 3-18-15, Kuramoto-cho Tokushima, Tokushima 770-8504, Japan. Phone: 81-886-33-7340. Fax: +81-886-33-7340. E-mail: yumoto{at}dent.dent.tokushima-u.ac.jp.

Infection and Immunity, January 1999, p. 384-394, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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