This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Krasan, G. P.
Right arrow Articles by St. Geme, J. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Krasan, G. P.
Right arrow Articles by St. Geme, J. W., III

 Previous Article  |  Next Article 

Infection and Immunity, January 1999, p. 449-454, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Adhesin Expression in Matched Nasopharyngeal and Middle Ear Isolates of Nontypeable Haemophilus influenzae from Children with Acute Otitis Media

Graham P. Krasan,1,2,* David Cutter,1,2 Stan L. Block,3 and Joseph W. St. Geme III1,2

Edward Mallinckrodt Department of Pediatrics and Department of Molecular Microbiology, Washington University School of Medicine,1 and Division of Infectious Diseases, St. Louis Children's Hospital,2 St. Louis, Missouri, and Pediatric Research Inc., Bardstown, Kentucky3

Received 24 June 1998/Returned for modification 19 August 1998/Accepted 15 October 1998

The HMW1 and HMW2 proteins, Hia, and hemagglutinating pili are important adherence factors in nontypeable Haemophilus influenzae. To gain insight into the relative importance of these adhesins in nasopharyngeal colonization and localized respiratory tract disease, we assessed their expression in matched nasopharyngeal and middle ear isolates of nontypeable H. influenzae from 17 children with acute otitis media. In all patients, including 11 with bilateral disease, the matched isolates were isogenic based on total protein profiles and genomic fingerprints. Of the nasopharyngeal isolates, 14 expressed only HMW1/HMW2-like proteins, 1 expressed only Hia, 1 expressed only pili, and 1 expressed both Hia and pili. Further analysis revealed concordance between nasopharyngeal isolates and the matched middle ear isolates for expression of the HMW1/HMW2-like proteins and Hia. In contrast, in the two children whose nasopharynges were colonized by piliated organisms, the corresponding middle ear isolates were nonpiliated and could not be enriched for piliation. Nevertheless, Southern analysis revealed that these two middle ear isolates contained all five hif genes required for pilus biogenesis and had no evidence of major genetic rearrangement. In summary, the vast majority of isolates of nontypeable H. influenzae associated with acute otitis media express HMW1/HMW2-like proteins, with expression present in both the nasopharynx and the middle ear. A smaller fraction of nasopharyngeal isolates express pili, while isogenic strains recovered from the middle ear are often refractory to enrichment for piliation. We speculate that the HMW adhesins and Hia are important at multiple steps in the pathogenesis of otitis media while pili contribute to early colonization and then become dispensable.

* Corresponding author. Mailing address: Edward Mallinckrodt Department of Pediatrics and Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Ave., Box 8230, St. Louis, MO 63110. Phone: (314) 362-5401. Fax: (314) 362-1232. E-mail: krasan_g{at}kids.wustl.edu.

Infection and Immunity, January 1999, p. 449-454, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Cholon, D. M., Cutter, D., Richardson, S. K., Sethi, S., Murphy, T. F., Look, D. C., St. Geme, J. W. III (2008). Serial Isolates of Persistent Haemophilus influenzae in Patients with Chronic Obstructive Pulmonary Disease Express Diminishing Quantities of the HMW1 and HMW2 Adhesins. Infect. Immun. 76: 4463-4468 [Abstract] [Full Text]  
  • Erwin, A. L., Sandstedt, S. A., Bonthuis, P. J., Geelhood, J. L., Nelson, K. L., Unrath, W. C. T., Diggle, M. A., Theodore, M. J., Pleatman, C. R., Mothershed, E. A., Sacchi, C. T., Mayer, L. W., Gilsdorf, J. R., Smith, A. L. (2008). Analysis of Genetic Relatedness of Haemophilus influenzae Isolates by Multilocus Sequence Typing. J. Bacteriol. 190: 1473-1483 [Abstract] [Full Text]  
  • McCrea, K. W., Xie, J., LaCross, N., Patel, M., Mukundan, D., Murphy, T. F., Marrs, C. F., Gilsdorf, J. R. (2008). Relationships of Nontypeable Haemophilus influenzae Strains to Hemolytic and Nonhemolytic Haemophilus haemolyticus Strains. J. Clin. Microbiol. 46: 406-416 [Abstract] [Full Text]  
  • Juliao, P. C., Marrs, C. F., Xie, J., Gilsdorf, J. R. (2007). Histidine Auxotrophy in Commensal and Disease-Causing Nontypeable Haemophilus influenzae. J. Bacteriol. 189: 4994-5001 [Abstract] [Full Text]  
  • Xie, J., Juliao, P. C., Gilsdorf, J. R., Ghosh, D., Patel, M., Marrs, C. F. (2006). Identification of New Genetic Regions More Prevalent in Nontypeable Haemophilus influenzae Otitis Media Strains than in Throat Strains. J. Clin. Microbiol. 44: 4316-4325 [Abstract] [Full Text]  
  • Giufre, M., Muscillo, M., Spigaglia, P., Cardines, R., Mastrantonio, P., Cerquetti, M. (2006). Conservation and Diversity of HMW1 and HMW2 Adhesin Binding Domains among Invasive Nontypeable Haemophilus influenzae Isolates. Infect. Immun. 74: 1161-1170 [Abstract] [Full Text]  
  • Chin, C. L., Manzel, L. J., Lehman, E. E., Humlicek, A. L., Shi, L., Starner, T. D., Denning, G. M., Murphy, T. F., Sethi, S., Look, D. C. (2005). Haemophilus influenzae from Patients with Chronic Obstructive Pulmonary Disease Exacerbation Induce More Inflammation than Colonizers. Am. J. Respir. Crit. Care Med. 172: 85-91 [Abstract] [Full Text]  
  • Ecevit, I. Z., McCrea, K. W., Marrs, C. F., Gilsdorf, J. R. (2005). Identification of New hmwA Alleles from Nontypeable Haemophilus influenzae. Infect. Immun. 73: 1221-1225 [Abstract] [Full Text]  
  • Ecevit, I. Z., McCrea, K. W., Pettigrew, M. M., Sen, A., Marrs, C. F., Gilsdorf, J. R. (2004). Prevalence of the hifBC, hmw1A, hmw2A, hmwC, and hia Genes in Haemophilus influenzae Isolates. J. Clin. Microbiol. 42: 3065-3072 [Abstract] [Full Text]  
  • Buscher, A. Z., Burmeister, K., Barenkamp, S. J., St. Geme, J. W. III (2004). Evolutionary and Functional Relationships among the Nontypeable Haemophilus influenzae HMW Family of Adhesins. J. Bacteriol. 186: 4209-4217 [Abstract] [Full Text]  
  • O'Neill, J. M., St. Geme III, J. W., Cutter, D., Adderson, E. E., Anyanwu, J., Jacobs, R. F., Schutze, G. E. (2003). Invasive Disease Due to Nontypeable Haemophilus influenzae among Children in Arkansas. J. Clin. Microbiol. 41: 3064-3069 [Abstract] [Full Text]  
  • Pettigrew, M. M., Foxman, B., Marrs, C. F., Gilsdorf, J. R. (2002). Identification of the Lipooligosaccharide Biosynthesis Gene lic2B as a Putative Virulence Factor in Strains of Nontypeable Haemophilus influenzae That Cause Otitis Media. Infect. Immun. 70: 3551-3556 [Abstract] [Full Text]  
  • Pettigrew, M. M., Foxman, B., Ecevit, Z., Marrs, C. F., Gilsdorf, J. (2002). Use of Pulsed-Field Gel Electrophoresis, Enterobacterial Repetitive Intergenic Consensus Typing, and Automated Ribotyping To Assess Genomic Variability among Strains of Nontypeable Haemophilus influenzae. J. Clin. Microbiol. 40: 660-662 [Abstract] [Full Text]  
  • Nelson, K. M., Young, G. M., Miller, V. L. (2001). Identification of a Locus Involved in Systemic Dissemination of Yersinia enterocolitica. Infect. Immun. 69: 6201-6208 [Abstract] [Full Text]  
  • Henderson, I. R., Nataro, J. P. (2001). Virulence Functions of Autotransporter Proteins. Infect. Immun. 69: 1231-1243 [Full Text]  
  • Cerquetti, M., Ciofi degli Atti, M. L., Renna, G., Tozzi, A. E., Garlaschi, M. L., Mastrantonio, P., The Hi Study Group, (2000). Characterization of Non-Type B Haemophilus influenzae Strains Isolated from Patients with Invasive Disease. J. Clin. Microbiol. 38: 4649-4652 [Abstract] [Full Text]  
  • St. Geme, J. W. III, Cutter, D. (2000). The Haemophilus influenzae Hia Adhesin Is an Autotransporter Protein That Remains Uncleaved at the C Terminus and Fully Cell Associated. J. Bacteriol. 182: 6005-6013 [Abstract] [Full Text]  
  • BROWN, N. F., BEACHAM, I. R. (2000). Cloning and analysis of genomic differences unique to Burkholderia pseudomallei by comparison with B. thailandensis. J Med Microbiol 49: 993-1001 [Abstract] [Full Text]  
  • van Schilfgaarde, M., van Ulsen, P., Eijk, P., Brand, M., Stam, M., Kouame, J., van Alphen, L., Dankert, J. (2000). Characterization of Adherence of Nontypeable Haemophilus influenzae to Human Epithelial Cells. Infect. Immun. 68: 4658-4665 [Abstract] [Full Text]  
  • Kubiet, M., Ramphal, R., Weber, A., Smith, A. (2000). Pilus-Mediated Adherence of Haemophilus influenzae to Human Respiratory Mucins. Infect. Immun. 68: 3362-3367 [Abstract] [Full Text]  
  • Frick, A. G., Joseph, T. D., Pang, L., Rabe, A. M., St. Geme, J. W. III, Look, D. C. (2000). Haemophilus influenzae Stimulates ICAM-1 Expression on Respiratory Epithelial Cells. J. Immunol. 164: 4185-4196 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»