This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Biswas, G. D.
Right arrow Articles by Sparling, P. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Biswas, G. D.
Right arrow Articles by Sparling, P. F.

 Previous Article  |  Next Article 

Infection and Immunity, January 1999, p. 455-459, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification and Functional Characterization of the Neisseria gonorrhoeae lbpB Gene Product

Gour D. Biswas,1,* James E. Anderson,1 Ching-Ju Chen,1 Cynthia Nau Cornelissen,2 and P. Frederick Sparling1,3

Department of Medicine1 and Department of Microbiology and Immunology,3 School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, and Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia 232982

Received 10 July 1998/Returned for modification 18 August 1998/Accepted 20 October 1998

We cloned lbpB, encoding a predicted 80-kDa lipoprotein, upstream of lbpA. A nonpolar mutant (LbpB- LbpA+) had normal lactoferrin (LF) binding and grew normally with LF as an iron source, whereas LbpB- LbpA- and LbpB+ LbpA- strains had reduced binding of LF and did not grow with LF as an iron source. LbpB bound LF directly in an affinity purification, suggesting that LbpB might play a still-uncharacterized role in the LF iron utilization.


* Corresponding author. Mailing address: Department of Medicine, University of North Carolina at Chapel Hill, CB# 7030, Chapel Hill, NC 27599-7005. Phone: (919) 966-3661. Fax: (919) 966-6714. E-mail: gdbis{at}med.unc.edu.


Infection and Immunity, January 1999, p. 455-459, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Ducey, T. F., Carson, M. B., Orvis, J., Stintzi, A. P., Dyer, D. W. (2005). Identification of the Iron-Responsive Genes of Neisseria gonorrhoeae by Microarray Analysis in Defined Medium. J. Bacteriol. 187: 4865-4874 [Abstract] [Full Text]  
  • Yost-Daljev, M. K., Cornelissen, C. N. (2004). Determination of Surface-Exposed, Functional Domains of Gonococcal Transferrin-Binding Protein A. Infect. Immun. 72: 1775-1785 [Abstract] [Full Text]  
  • Kenney, C. D., Cornelissen, C. N. (2002). Demonstration and Characterization of a Specific Interaction between Gonococcal Transferrin Binding Protein A and TonB. J. Bacteriol. 184: 6138-6145 [Abstract] [Full Text]  
  • Masri, H. P., Cornelissen, C. N. (2002). Specific Ligand Binding Attributable to Individual Epitopes of Gonococcal Transferrin Binding Protein A. Infect. Immun. 70: 732-740 [Abstract] [Full Text]  
  • Chen, C.-J., Mclean, D., Thomas, C. E., Anderson, J. E., Sparling, P. F. (2002). Point Mutations in HpuB Enable Gonococcal HpuA Deletion Mutants To Grow on Hemoglobin. J. Bacteriol. 184: 420-426 [Abstract] [Full Text]  
  • Ronpirin, C., Jerse, A. E., Cornelissen, C. N. (2001). Gonococcal Genes Encoding Transferrin-Binding Proteins A and B Are Arranged in a Bicistronic Operon but Are Subject to Differential Expression. Infect. Immun. 69: 6336-6347 [Abstract] [Full Text]  
  • Desai, P. J., Garges, E., Genco, C. A. (2000). Pathogenic Neisseriae Can Use Hemoglobin, Transferrin, and Lactoferrin Independently of the tonB Locus. J. Bacteriol. 182: 5586-5591 [Abstract] [Full Text]  
  • Cornelissen, C. N., Anderson, J. E., Boulton, I. C., Sparling, P. F. (2000). Antigenic and Sequence Diversity in Gonococcal Transferrin-Binding Protein A. Infect. Immun. 68: 4725-4735 [Abstract] [Full Text]  
  • Carson, S. D. B., Klebba, P. E., Newton, S. M. C., Sparling, P. F. (1999). Ferric Enterobactin Binding and Utilization by Neisseria gonorrhoeae. J. Bacteriol. 181: 2895-2901 [Abstract] [Full Text]