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Infection and Immunity, January 1999, p. 50-56, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Characterization of emb, a Gene Encoding the Major Adhesin of Streptococcus defectivus

Riccardo Manganelli,dagger and Ivo van de Rijn*

Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston-Salem, North Carolina

Received 22 June 1998/Returned for modification 11 September 1998/Accepted 6 October 1998

Streptococcus defectivus is one of the nutritionally variant streptococci, a class of viridans group streptococci first isolated from patients with endocarditis and otitis media. In previous studies, NVS-47, a clinical isolate of S. defectivus, was shown to bind to the extracellular matrix. A high-molecular-weight surface protein was identified and proposed to be responsible for mediating this binding. In the present study, the gene encoding this protein was identified by transposon mutagenesis and characterized. The gene (emb) was found to be larger than 14 kb and was partially sequenced. It encodes a protein containing at least 50 repeats of 77 amino acids predicted to assume an alternating coiled-coil conformation. The domain responsible for extracellular matrix binding was mapped to the N terminus of the protein. From sequence analysis, Emb is proposed to be the prototype of a new family of streptococcal fibrillar proteins.

* Corresponding author. Mailing address: Wake Forest University School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157. Phone: (336) 716-2263. Fax: (336) 716-4204. E-mail: ivr{at}wfubmc.edu.

dagger Present address: Department of Microbiology, Public Health Research Institute, New York, N.Y.

Infection and Immunity, January 1999, p. 50-56, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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