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Infection and Immunity, October 1999, p. 5001-5006, Vol. 67, No. 10
Channing Laboratory, Department of Medicine,
Brigham and Women's Hospital and Harvard Medical School, Boston,
Massachusetts 02115,1 and Data
Coordinating Center, Boston University School of Public Health,
Boston, Massachusetts 021182
Received 2 March 1999/Returned for modification 4 May 1999/Accepted 29 July 1999
Staphylococcus aureus nasal carriage is a risk factor
for infection in humans, particularly in the hospital environment.
Attenuation of carriage has proven effective in reducing the prevalence
of infection in some high-risk groups. To study staphylococcal factors that influence nasal colonization, a mouse model of S. aureus nasal colonization was developed. Mice were inoculated
intranasally with S. aureus Reynolds, and nasal carriage
was evaluated by quantitating cultures of the nasal tissues from mice
sacrificed at various time points after inoculation. The majority of
mice inoculated with 108 CFU of S. aureus
maintained nasal carriage for at least 20 days. Nasal colonization
rates were similar for inbred (BALB/c and C57BL/6) and outbred (ICR)
mice. Colonization was not affected by mouse passage of strain
Reynolds. Lower inoculum doses (<107 CFU) resulted in
reduced colonization after 7 days. However, mice given streptomycin in
their drinking water developed long-term carriage of S. aureus, and they were colonized with inocula as low as
105 CFU. Nasal colonization was also established with two
other S. aureus strains (one strain each of human and
murine origins). S. aureus recovered from the nares of
experimentally colonized mice expressed high levels of capsule, and the
ability of a capsule-defective mutant to persist in the nares was
reduced in comparison to that of the parent strain. This nasal
colonization model should prove useful for studies of factors that
mediate S. aureus colonization and for assessment of
targets for antimicrobial intervention or vaccine development.
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Development and Characterization of a
Staphylococcus aureus Nasal Colonization Model in
Mice
*
Corresponding author. Mailing address: Channing
Laboratory, 181 Longwood Ave., Boston, MA 02115-5899. Phone: (617)
525-2652. Fax: (617) 731-1541. E-mail:
jean.lee{at}channing.harvard.edu.
Infection and Immunity, October 1999, p. 5001-5006, Vol. 67, No. 10
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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