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Infection and Immunity, October 1999, p. 5007-5011, Vol. 67, No. 10
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Recombinant Staphylococcus Strains as Live Vectors for the Induction of Neutralizing Anti-Diphtheria Toxin Antisera

Cécile Fromen-Romano,1 Pascal Drevet,1 Alain Robert,2 André Ménez,1 and Michel Léonetti1,*

CEA, Département d'Ingénierie et d'Etudes des Protéines (DIEP), Centre d'Etude de Saclay, 91191 Gif-Sur-Yvette Cedex,1 and Centre d'Immunologie Pierre Fabre (CIPF), 74164 Saint-Julien en Genevois,2 France

Received 8 March 1999/Returned for modification 20 May 1999/Accepted 7 July 1999

We have investigated whether the nonpathogenic gram-positive bacteria Staphylococcus xylosus and S. carnosus can display a whole domain of a toxic protein on their surface and if such vectors are suitable for immunization of BALB/c mice. The nucleotide sequence encoding the receptor-binding domain (DTR; amino acids 382 to 535) of diphtheria toxin (DT) was inserted into plasmids pSE'mp18ABPXM and pSPPmABPXM, which were designed to display heterologous proteins on S. xylosus and S. carnosus cell surfaces, respectively. Western blot analysis of the resulting bacterial lysates indicates that DTR is produced by each expression system. However, analysis of rabbit anti-DTR antisera binding to the transformed live bacteria shows that DTR is not displayed on the surface of S. xylosus cells whereas it is efficiently exposed on S. carnosus. A significant anti-DT antibody response was raised in BALB/c mice immunized intraperitoneally with S. carnosus displaying DTR, and the antisera abolished DT cytotoxicity on Vero cells. Thus, only S. carnosus can display a whole domain of a toxic protein and represents a potential vector for humoral vaccination.

* Corresponding author. Mailing address: CEA, Département d'Ingénierie et d'Etudes des Protéines (DIEP), Centre d'Etude de Saclay, 91191 Gif-Sur-Yvette Cedex, France. Phone: (33) 01 69 08 64 56. Fax: (33) 01 69 08 90 71. E-mail: leonetti{at}cea.fr.

Infection and Immunity, October 1999, p. 5007-5011, Vol. 67, No. 10
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.