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Infection and Immunity, October 1999, p. 5247-5252, Vol. 67, No. 10
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Kinetics and Mechanisms of Extracellular Protein Release by Helicobacter pylori

Wayne Schraw,1 Mark S. McClain,1 and Timothy L. Cover1,2,3,*

Division of Infectious Diseases, Department of Medicine,1 and Department of Microbiology and Immunology,2 Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2605, and Department of Veterans Affairs Medical Center, Nashville, Tennessee 37232-26373

Received 9 April 1999/Returned for modification 24 May 1999/Accepted 16 July 1999

To investigate the kinetics and mechanisms of extracellular protein release by Helicobacter pylori, we analyzed the entry of metabolically radiolabeled bacterial proteins into broth culture supernatant. At early time points, vacuolating cytotoxin (VacA) constituted a major extracellular protein. Subsequently, culture supernatants accumulated many proteins that were components of intact bacterial cells. This nonselective release of proteins was associated with a decreasing turbidity of cultures and loss of bacterial viability, indicative of an autolytic process. The rates of VacA secretion and autolysis were each influenced by medium composition, and therefore these may be regulated phenomena. Extracellular release of proteins by H. pylori may be an important adaptation that facilitates the persistence of H. pylori in the human gastric mucus layer. Moreover, entry of proinflammatory proteins into the gastric mucosa may contribute to the induction of a mucosal inflammatory response.

* Corresponding author. Mailing address: Division of Infectious Diseases, A3310 Medical Center North, Vanderbilt University School of Medicine, Nashville, TN 37232-2605. Phone: (615) 322-2035. Fax: (615) 343-6160. E-mail: COVERTL{at}ctrvax.vanderbilt.edu.

Infection and Immunity, October 1999, p. 5247-5252, Vol. 67, No. 10
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.