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Infection and Immunity, October 1999, p. 5275-5281, Vol. 67, No. 10
Department of Veterinary PathoBiology,
University of Minnesota, St. Paul, Minnesota 55108
Received 29 April 1999/Returned for modification 9 June
1999/Accepted 27 July 1999
The ability of innate immune cells to differentially respond to
various bacterial components provides a mechanism by which the acquired
immune response may be tailored to specific pathogens. The response of
innate immune cells to bacterial components provides regulatory signals
to cognate immune cells. These signals include secreted cytokines and
costimulatory molecules, and to a large extent they determine the
quantitative and qualitative nature of the immune response. In order to
determine if innate immune cells can differentially respond to
bacterial components, we compared the responses of macrophages to two
bacterially derived molecules, cholera toxin (CT) and
lipopolysaccharide (LPS). We found that CT and LPS differentially
regulated the expression of interleukin-12 (IL-12) and CD80-CD86 but
not that of IL-1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Differential Regulation of Macrophage Interleukin-1
(IL-1), IL-12, and CD80-CD86 by Two Bacterial Toxins
. LPS and CT each induced IL-1 expression in
macrophages, while only LPS induced IL-12 and only CT induced
CD80-CD86. These differences were markedly potentiated in gamma
interferon (IFN-
)-treated macrophages, in which LPS potently induced
IL-12 and CD80-CD86 expression. In contrast, IFN- treatment had no
effect on the expression of IL-1. These results define a molecular
basis for the differential pathogenicities of bacterial toxins and are
relevant to the design of vaccine adjuvants able to selectively induce
desired types of immunity.
*
Corresponding author. Mailing address: Department of
Veterinary PathoBiology, University of Minnesota, 205 VSB, 1971 Commonwealth Ave., St. Paul, MN 55108. Phone: (612) 624-4926. Fax:
(612) 625-5203. E-mail: fossx005{at}tc.umn.edu.
Infection and Immunity, October 1999, p. 5275-5281, Vol. 67, No. 10
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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