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Infection and Immunity, October 1999, p. 5470-5472, Vol. 67, No. 10
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

An OspC-Specific Monoclonal Antibody Passively Protects Mice from Tick-Transmitted Infection by Borrelia burgdorferi B31

M. Lamine Mbow,1,* Robert D. Gilmore Jr.,2 and Richard G. Titus1

Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University,1 and Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services,2 Fort Collins, Colorado

Received 31 March 1999/Returned for modification 11 May 1999/Accepted 21 July 1999

A murine monoclonal antibody directed against Borrelia burgdorferi B31 outer surface protein C (OspC) antigen was generated by a method whereby borreliae were inoculated into the mouse via the natural transmission mode of tick feeding. Passive immunization with this antibody resulted in protection of C3H/HeJ and outbred mice from a tick-transmitted challenge infection. Immunofluorescence staining of borrelia cells indicated surface exposure of the OspC epitope reactive with the monoclonal antibody.

* Corresponding author. Mailing address: Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523-1671. Phone: (970) 491-1607. Fax: (970) 491-0603. E-mail: lmbow{at}lamar.colostate.edu.

Infection and Immunity, October 1999, p. 5470-5472, Vol. 67, No. 10
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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