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Infection and Immunity, November 1999, p. 5547-5551, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Carrier Properties of a Protein Derived from Outer Membrane Protein A of Klebsiella pneumoniae

Isabelle Rauly, Liliane Goetsch, Jean-François Haeuw, Christine Tardieux, Thierry Baussant, Jean-Yves Bonnefoy, and Nathalie Corvaia*

Centre d'Immunologie Pierre Fabre, Saint Julien en Genevois, France

Received 20 January 1999/Returned for modification 6 April 1999/Accepted 6 August 1999

We have recently cloned a new protein, recombinant P40 (rP40). When tested in vivo after conjugation to a B-cell epitope, rP40 induces an important antibody response without the need for adjuvant. To characterize its potency, this carrier protein was coupled to a peptide derived from respiratory syncytial virus attachment G protein (G1'). After immunization of mice with the rP40-G1' conjugate, strong antipeptide antibodies were detected, whereas peptide alone was not immunogenic. To emphasize the carrier properties of rP40, a polysaccharide derived from Haemophilus influenzae type b (Hib) was coupled to it. Immunoglobulin G responses against the Hib polysaccharide were observed after coupling to rP40. Interestingly, an antipeptide antibody response was observed despite preexisting anti-rP40 antibodies generated by preimmunization with rP40. In addition, rP40 compares well with the reference carrier protein, tetanus toxoid (TT), since antibody responses of equal intensity were observed when a peptide or a polysaccharide was coupled to TT and rP40. Moreover, rP40 had advantages compared to TT; e.g., it induced a mixed Th1/Th2 response, whereas TT induced only a Th2 profile. Together, the results indicate that rP40 is a novel carrier protein with potential for use as an alternative carrier for human vaccination.

* Corresponding author. Mailing address: Centre d'Immunologie Pierre Fabre, Saint Julien en Genevois, France. Phone: 33-4-50-35-35-74. Fax: 33-4-50-35-35-90. E-mail: nathalie.corvaia{at}pierre.fabre.com.

Infection and Immunity, November 1999, p. 5547-5551, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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