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Infection and Immunity, November 1999, p. 5651-5657, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Analysis of Virulence of Clinical Isolates of Salmonella enteritidis In Vivo and In Vitro

Sangwei Lu,1 Amee R. Manges,1 Yisheng Xu,2 Ferric C. Fang,2 and Lee W. Riley1,*

Program in Infectious Diseases and Immunity, School of Public Health, University of California at Berkeley, Berkeley, California,1 and Department of Medicine, Division of Infectious Diseases, University of Colorado Health Sciences Center, Denver, Colorado2

Received 17 May 1999/Returned for modification 5 August 1999/Accepted 9 August 1999

Salmonella enterica serotype Enteritidis (S. enteritidis) is a major food-borne pathogen, and its incidence among all Salmonella serotypes has increased dramatically in the last two decades. To study the virulence characteristics of clinical isolates of S. enteritidis, we determined the 50% lethal doses (LD50) in mice of isolates of two major phage types (4 and 8). Isolates of both phage types showed a wide range of LD50 after oral inoculation, varying from under 102 organisms to over 108 organisms. No significant difference in LD50 was observed between the phage types. These observations indicated that clinical isolates of S. enteritidis are highly heterogeneous in their ability to cause death in mice. We compared the LD50s of these isolates to the results observed from in vitro pathogenicity assays. We also analyzed these isolates for recognized Salmonella virulence loci (spv, sodCI, sopE, and sef). The in vitro phenotypes of the isolates showed no obvious correlation with their LD50 in any given assay, and the virulence genes tested were present in all isolates. However, the isolate with the lowest LD50 (isolate 97A 2472) was resistant to acidified sodium nitrite (ASN). Moreover, the most acid-susceptible, macrophage-susceptible, and ASN-susceptible isolates were attenuated for virulence in mice. These results, based on extensive analysis of clinical isolates of S. enteritidis, demonstrate the complex nature of Salmonella pathogenesis in mice. Our results also indicate the limitation of in vitro assays in predicting in vivo virulence.

* Corresponding author. Mailing address: 140 Warren Hall, School of Public Health, University of California at Berkeley, Berkeley, CA 94720. Phone: (510) 642-9200. Fax: (510) 642-6350. E-mail: lwriley{at}uclink4.berkeley.edu.

Infection and Immunity, November 1999, p. 5651-5657, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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