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Infection and Immunity, November 1999, p. 5946-5950, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Recognition of Schistosome Glycolipids by Immunoglobulin E: Possible Role in Immunity

Desiree van der Kleij,1,2 Aloysius G. M. Tielens,2 and Maria Yazdanbakhsh1,*

Department of Parasitology, Leiden University Medical Center, Leiden,1 and Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht,2 The Netherlands

Received 22 March 1999/Returned for modification 17 May 1999/Accepted 16 August 1999

To investigate the role of antibody responses to (glyco)lipids in immunity to schistosome infection, lipids extracted from Schistosoma mansoni eggs and adult worms were fractionated, and the antibody isotype profile reactive to the fractionated lipids in a well-characterized S. haematobium-infected population was investigated. In tests of 10 plasma samples it was found that immunoglobulin G (IgG) reactivity was highest to the fraction containing ceramidepolyhexosides, whereas IgE reactivity was most prominent to both cerebroside- and ceramidepolyhexoside-containing fractions. The fraction containing ceramidepolyhexosides was then tested for reactivity with IgG subclasses and IgE in plasma samples from 66 S. haematobium-infected patients. Considering IgG4 and IgE, isotypes of particular interest in helminth infections, we found that both isotypes recognized egg (glyco)proteins in more than 90% of the infected subjects. However, in the case of glycolipids, IgE reactivity was much more prominent than IgG4 reactivity (found in 80 and 41% of the subjects, respectively). Furthermore, worm glycolipid-specific IgE prior to treatment of the subjects with praziquantel was negatively correlated with egg counts at 2 years posttreatment, indicating that IgE directed towards glycolipids could play an important role in resistance to reinfection.

* Corresponding author. Mailing address: Dept. of Parasitology, Leiden University Medical Center, P.O. Box 9605, 2300 RC Leiden, The Netherlands. Phone: 31 71 5276861. Fax: 31 71 5276850. E-mail: maria{at}rullf2.MedFac.LeidenUniv.nl.

Infection and Immunity, November 1999, p. 5946-5950, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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