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Infection and Immunity, November 1999, p. 5972-5978, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Bordetella bronchiseptica-Mediated Cytotoxicity to Macrophages Is Dependent on bvg-Regulated Factors, Including Pertactin

Catrina B. Forde,1 Xiaoju Shi,1,dagger Jingli Li,2 and Mark Roberts1,*

Department of Veterinary Pathology, University of Glasgow Veterinary School, Garscube Estate, Glasgow G61 1QH, Scotland,1 and Vaccine Research Unit, Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ, England2

Received 12 April 1999/Returned for modification 26 May 1999/Accepted 30 August 1999

The effect of Bordetella bronchiseptica infection on the viability of murine macrophage-like cells and on primary porcine alveolar macrophages was investigated. The bacterium was shown to be cytotoxic for both cell types, particularly where tight cell-to-cell contacts were established. In addition, bvg mutants were poorly cytotoxic for the eukaryotic cells, while a prn mutant was significantly less toxic than wild-type bacteria. B. bronchiseptica-mediated cytotoxicity was inhibited in the presence of cytochalasin D or cycloheximide, an inhibitor of microfilament-dependent phagocytosis or de novo eukaryotic protein synthesis, respectively. The mechanism of eukaryotic cell death was examined, and cell death was found to occur primarily through a necrotic pathway, although a small proportion of the population underwent apoptosis.

* Corresponding author. Mailing address: Department of Veterinary Pathology, University of Glasgow Veterinary School, Garscube Estate, Bearsden Rd., Glasgow G61 1QH, Scotland. Phone: 141 330 5780. Fax: 141 330 5602. E-mail: m.roberts{at}vet.gla.ac.uk.

dagger Present address: Department of Immunology, St. Bartholomew's and The Royal London School of Medicine and Dentistry, London EC1A 7BE, England.

Infection and Immunity, November 1999, p. 5972-5978, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 1999 by the American Society for Microbiology. All rights reserved.