This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Veltrop, M. H. A. M.
Right arrow Articles by Thompson, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Veltrop, M. H. A. M.
Right arrow Articles by Thompson, J.

 Previous Article  |  Next Article 

Infection and Immunity, November 1999, p. 6130-6138, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Bacterial Species- and Strain-Dependent Induction of Tissue Factor in Human Vascular Endothelial Cells

M. H. A. M. Veltrop, H. Beekhuizen,* and J. Thompson

Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands

Received 28 May 1999/Returned for modification 22 July 1999/Accepted 31 August 1999

A cardinal process in bacterial endocarditis (BE) is the activation of the clotting system and the formation of a fibrin clot on the inner surface of the heart, the so-called endocardial vegetation. The processes that lead to the activation of the clotting system on endothelial surfaces upon exposure to bacteria are largely unknown. In the present study, we investigated in an in vitro model whether infection of human endothelial cells (EC) with bacteria that are relevant to BE, such as Staphylococcus aureus, Streptococcus sanguis, and Staphylococcus epidermidis, leads to induction of tissue factor (TF)-dependent procoagulant activity (TFA) and whether this process is influenced by host factors, such as interleukin-1 (IL-1), that are produced in response to the bacteremia in vivo. The results show that S. aureus binds to and is internalized by EC, resulting in expression of TF mRNA and TF surface protein as well as generation of TFA within 4 to 8 h after infection. No TFA was found when EC were exposed to UV-irradiated S. aureus or bacterial cell wall fragments. S. sanguis and S. epidermidis, although also binding to EC, did not induce endothelial TFA. This indicates a species and strain dependency. EC also expressed TFA after exposure to IL-1. The enhanced TFA of EC after exposure to S. aureus was not prevented by IL-1 receptor antagonist, arguing against an auto- or paracrine contribution of endogenous IL-1. When IL-1 was applied together with bacteria, this had a synergistic effect on the induction of EC TFA. This was found in particular with S. aureus but also, although to a lesser degree, with S. sanguis and S. epidermidis. This influence of IL-1 on the species- and strain-dependent induction of EC TFA suggests that bacterial factors as well as host factors orchestrate the induction of coagulation in an early stage in the pathogenesis of endovascular disease, such as BE.

* Corresponding author. Mailing address: Department of Infectious Diseases, C5-P, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The Netherlands. Phone: 31-71-5261784 or 31-71-5262613. Fax: 31-71-5266758. E-mail: beekhuiz{at}stad.dsl.nl.

Infection and Immunity, November 1999, p. 6130-6138, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Chuang, O. N., Schlievert, P. M., Wells, C. L., Manias, D. A., Tripp, T. J., Dunny, G. M. (2009). Multiple Functional Domains of Enterococcus faecalis Aggregation Substance Asc10 Contribute to Endocarditis Virulence. Infect. Immun. 77: 539-548 [Abstract] [Full Text]  
  • Beekhuizen, H., van de Gevel, J. S. (2007). Gamma Interferon Confers Resistance to Infection with Staphylococcus aureus in Human Vascular Endothelial Cells by Cooperative Proinflammatory and Enhanced Intrinsic Antibacterial Activities. Infect. Immun. 75: 5615-5626 [Abstract] [Full Text]  
  • Hahn, C.-L., Best, A.M., Tew, J.G. (2007). Rapid Tissue Factor Induction by Oral Streptococci and Monocyte-IL-1{beta}. JDR 86: 255-259 [Abstract] [Full Text]  
  • Que, Y.-A., Haefliger, J.-A., Piroth, L., Francois, P., Widmer, E., Entenza, J. M., Sinha, B., Herrmann, M., Francioli, P., Vaudaux, P., Moreillon, P. (2005). Fibrinogen and fibronectin binding cooperate for valve infection and invasion in Staphylococcus aureus experimental endocarditis. JEM 201: 1627-1635 [Abstract] [Full Text]  
  • Bezerra, L. M. L., Filler, S. G. (2004). Interactions of Aspergillus fumigatus with endothelial cells: internalization, injury, and stimulation of tissue factor activity. Blood 103: 2143-2149 [Abstract] [Full Text]  
  • Bryant, A. E. (2003). Biology and Pathogenesis of Thrombosis and Procoagulant Activity in Invasive Infections Caused by Group A Streptococci and Clostridium perfringens. Clin. Microbiol. Rev. 16: 451-462 [Abstract] [Full Text]  
  • Bryant, A. E., Hayes-Schroer, S. M., Stevens, D. L. (2003). M Type 1 and 3 Group A Streptococci Stimulate Tissue Factor-Mediated Procoagulant Activity in Human Monocytes and Endothelial Cells. Infect. Immun. 71: 1903-1910 [Abstract] [Full Text]  
  • Mattsson, E., Herwald, H., Bjorck, L., Egesten, A. (2002). Peptidoglycan from Staphylococcus aureus Induces Tissue Factor Expression and Procoagulant Activity in Human Monocytes. Infect. Immun. 70: 3033-3039 [Abstract] [Full Text]  
  • Veltrop, M. H. A. M., Thompson, J., Beekhuizen, H. (2001). Monocytes Augment Bacterial Species- and Strain-Dependent Induction of Tissue Factor Activity in Bacterium-Infected Human Vascular Endothelial Cells. Infect. Immun. 69: 2797-2807 [Abstract] [Full Text]  
  • Veltrop, M. H. A. M., Langermans, J. A. M., Thompson, J., Bancsi, M. J. L. M. F. (2001). Interleukin-10 Regulates the Tissue Factor Activity of Monocytes in an In Vitro Model of Bacterial Endocarditis. Infect. Immun. 69: 3197-3202 [Abstract] [Full Text]  
  • Tapper, H., Herwald, H. (2000). Modulation of hemostatic mechanisms in bacterial infectious diseases. Blood 96: 2329-2337 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»