Cell Death of Human Polymorphonuclear Neutrophils Induced by a Pseudomonas aeruginosa Cystic Fibrosis Isolate Requires a Functional Type III Secretion System

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Infection and Immunity, November 1999, p. 6164-6167, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cell Death of Human Polymorphonuclear Neutrophils Induced by a Pseudomonas aeruginosa Cystic Fibrosis Isolate Requires a Functional Type III Secretion System

D. Dacheux, I. Attree, C. Schneider, and B. Toussaint^*

Département de Biologie Moléculaire et Structurale, BBSI, UMR-314 CNRS, CEA Grenoble, Grenoble, France

Received 19 May 1999/Returned for modification 1 July 1999/Accepted 18 August 1999

With a coincubation model incorporating Pseudomonas aeruginosa and human polymorphonuclear neutrophils (PMNs), a cystic fibrosis(CF) P. aeruginosa isolate has been shown to resist the bactericidalaction of PMNs and to induce their cellular death. An isogenicmutant of this CF isolate in which the type III secretion systemwas rendered nonfunctional was unable to induce cellular deathofPMNs.

^* Corresponding author. Mailing address: DBMS/BBSI, CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 09, France. Phone:33.4.76.88.34.83. Fax: 33.4.76.88.51.85. E-mail: btoussaint{at}cea.fr.

Infection and Immunity, November 1999, p. 6164-6167, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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