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Infection and Immunity, November 1999, p. 6191-6193, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Syntheses and Immunologic Properties of Escherichia coli O157 O-Specific Polysaccharide and Shiga Toxin 1 B Subunit Conjugates in Mice

Edward Konadu,1 Arthur Donohue-Rolfe,2 Stephen B. Calderwood,3 Vince Pozsgay,1 Joseph Shiloach,4 John B. Robbins,1 and Shousun C. Szu1,*

National Institute of Child Health and Human Development1 and National Institute of Diabetes and Digestive and Kidney Diseases,4 National Institutes of Health, Bethesda, Maryland 20892-2720; Tufts University School of Veterinary Medicine, Medford, Massachusetts 015362; and Massachusetts General Hospital, Boston, Massachusetts 021143

Received 18 May 1999/Returned for modification 29 June 1999/Accepted 9 August 1999

Escherichia coli O157 is the major cause of diarrhea-associated hemolytic uremic syndrome (HUS). Strains causing HUS contain either Shiga toxin 1 (Stx1) or Stx2, or both. In adult volunteers, conjugate vaccines of detoxified lipopolysaccharide (LPS) elicited bactericidal antibodies to E. coli O157. Here, the detoxified LPS was conjugated with improved schemes to the nontoxic B subunit of Stx1. Mice injected with these bivalent conjugates elicited both bactericidal antibodies to E. coli O157 and neutralization antibodies to Stx1.

* Corresponding author. Mailing address: National Institute of Child Health and Human Development, Room 424, Building 6, National Institutes of Health, Bethesda, MD 20892-2720. Phone: (301) 496-1185. Fax: (301) 402-9108. E-mail: scszu{at}helix.nih.gov.

Infection and Immunity, November 1999, p. 6191-6193, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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