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Infection and Immunity, November 1999, p. 6194-6197, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Homotypic and Heterotypic Antibody Responses to a 56-Kilodalton Protein of Orientia tsutsugamushi

Myung-Sik Choi,1 Seung-Yong Seong,1 Jae-Seung Kang,2 Yun-Won Kim,3 Myung-Suk Huh,1 and Ik-Sang Kim1,*

Department of Microbiology, Seoul National University College of Medicine and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799,1 Department of Microbiology, College of Medicine, Inha University, Inchon 402-751,2 and Department of Microbiology, College of Medicine, Hallym University, Chunchon 200-702,3 Republic of Korea

Received 29 March 1999/Returned for modification 1 June 1999/Accepted 18 August 1999

We analyzed homotypic and heterotypic antibody responses to a type-specific antigen (Tsa), a 56-kDa protein of Orientia tsutsugamushi, by using sera from mice immunized with strains Gilliam, Karp, Kato, and Boryong. We generated a series of deletion constructs of the tsa gene and expressed them as MalE fusion proteins. Variable domain I (VD I) showed strong responses to homotypic antibodies. Antigenic domain II (AD II) from Boryong and Karp showed cross-reactivities to each other. VD III showed no responses to any of the antibodies. Sera from Kato-immunized mice showed only homotypic responses to AD III. On the other hand, sera of the mice immunized with Gilliam, Karp, or Boryong showed homotypic as well as heterotypic responses to this region. VD IV showed the strongest heterotypic antibody responses among the fragments tested. These data suggest that VD I is important in homotypic antibody responses and that AD II, AD III, and VD IV are important in heterotypic antibody responses of the mice to Tsa.

* Corresponding author. Mailing address: Department of Microbiology, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Republic of Korea. Phone: 82-2-740-8304. Fax: 82-2-743-0881. E-mail: molecule{at}plaza.snu.ac.kr.

Infection and Immunity, November 1999, p. 6194-6197, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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