This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gysin, J.
Right arrow Articles by Lépolard, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gysin, J.
Right arrow Articles by Lépolard, C.

 Previous Article  |  Next Article 

Infection and Immunity, December 1999, p. 6596-6602, Vol. 67, No. 12
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Ex Vivo Desequestration of Plasmodium falciparum-Infected Erythrocytes from Human Placenta by Chondroitin Sulfate A

J. Gysin,1,* B. Pouvelle,1 N. Fievet,2 A. Scherf,3 and C. Lépolard1

Unité de Parasitologie Expérimentale, Faculté de Médecine, Université de la Méditerranée (Aix-Marseille II), 13385 Marseille Cedex 5,1 and Unité de Biologie des Interactions Hôte-Parasite, CNRS URA 1960, Institut Pasteur, 75724 Paris Cedex 15,3 France, and Laboratoire de Parasitologie, OCEAC, Yaoundé, Cameroon2

Received 17 May 1999/Returned for modification 5 August 1999/Accepted 7 September 1999

We performed ex vivo experiments with Plasmodium falciparum-infected human placentas from primi- and multigravida women from Cameroon. All women, independent of their gravida status, had anti-chondroitin sulfate A (CSA) adhesion antibodies which cross-reacted with heterologous strains, such as FCR3 and Palo-Alto(FUP)1, which were selected for CSA binding. These antibodies, directed against the surface of infected erythrocytes obtained by flushing with CSA (IRBCCSA), were restricted to the immunoglobulin G3 isotypes. Massive desequestration of parasites was achieved with soluble CSA but not with anti-ICAM-1 and anti-CD36 monoclonal antibodies. All of the CSA-flushed parasites were analyzed immediately by using in vitro assays of binding to Saimiri brain endothelial cells (SBEC) expressing various adhesion receptors. Parasites derived from all six placentas displayed the CSA adhesion phenotype. However, only partial inhibition of adhesion was observed in the presence of soluble CSA or when Sc1D SBEC were treated with chondroitinase ABC. These results suggest that an additional adhesive molecule of IRBCCSA which binds to an unidentified receptor is present in the placenta. This new phenotype was lost once the parasites adapted to in vitro culture. We observed additional differences in the CSA adhesion phenotype between placental parasites and in vitro-cultured parasites panned on endothelial cells carrying CSA. The minimum size of fractionated CSA required for a significant inhibition of placental IRBCCSA adhesion to Sc1D cells was 1 to 2 kDa, which contrasts with the 4-kDa size necessary to reach equivalent levels of inhibition with panned IRBCCSA of this phenotype. All placental IRBCCSA cytoadhered to Sc17 SBEC, which express only the CSA receptor. Panning of IRBCCSA on these cells resulted in a significant quantitative increase of IRBC cytoadhering to the CSA of Sc1D cells but did not change their capacity for adhesion to CSA on normal placenta cryosections. Our results indicate that the CSA binding phenotype is heterogeneous and that several distinct genes may encode P. falciparum-CSA ligands with distinct binding properties.

* Corresponding author. Mailing address: Unité de Parasitologie Expérimentale, Faculté de Médecine, Université de la Méditerranée (Aix-Marseille II), 13385 Marseille Cedex 5, France. Phone: 4-91-32-46-33/35. Fax: 4-91-32-46-34. E-mail: gysin{at}medecine.univ-mrs.fr.

Infection and Immunity, December 1999, p. 6596-6602, Vol. 67, No. 12
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Francis, S. E., Malkov, V. A., Oleinikov, A. V., Rossnagle, E., Wendler, J. P., Mutabingwa, T. K., Fried, M., Duffy, P. E. (2007). Six Genes Are Preferentially Transcribed by the Circulating and Sequestered Forms of Plasmodium falciparum Parasites That Infect Pregnant Women. Infect. Immun. 75: 4838-4850 [Abstract] [Full Text]  
  • Madhunapantula, S. V., Achur, R. N., Gowda, D. C. (2007). Developmental Stage- and Cell Cycle Number-Dependent Changes in Characteristics of Plasmodium falciparum-Infected Erythrocyte Adherence to Placental Chondroitin-4-Sulfate Proteoglycan. Infect. Immun. 75: 4409-4415 [Abstract] [Full Text]  
  • Beeson, J. G., Andrews, K. T., Boyle, M., Duffy, M. F., Choong, E. K., Byrne, T. J., Chesson, J. M., Lawson, A. M., Chai, W. (2007). Structural Basis for Binding of Plasmodium falciparum Erythrocyte Membrane Protein 1 to Chondroitin Sulfate and Placental Tissue and the Influence of Protein Polymorphisms on Binding Specificity. J. Biol. Chem. 282: 22426-22436 [Abstract] [Full Text]  
  • Beeson, J. G., Ndungu, F., Persson, K. E. M., Chesson, J. M., Kelly, G. L., Uyoga, S., Hallamore, S. L., Williams, T. N., Reeder, J. C., Brown, G. V., Marsh, K. (2007). Antibodies among Men and Children to Placental-Binding Plasmodium falciparum-Infected Erythrocytes that Express var2csa. Am J Trop Med Hyg 77: 22-28 [Abstract] [Full Text]  
  • Bir, N., Yazdani, S. S., Avril, M., Layez, C., Gysin, J., Chitnis, C. E. (2006). Immunogenicity of Duffy Binding-Like Domains That Bind Chondroitin Sulfate A and Protection against Pregnancy-Associated Malaria.. Infect. Immun. 74: 5955-5963 [Abstract] [Full Text]  
  • Rasti, N., Namusoke, F., Chene, A., Chen, Q., Staalsoe, T., Klinkert, M.-Q., Mirembe, F., Kironde, F., Wahlgren, M. (2006). Nonimmune immunoglobulin binding and multiple adhesion characterize Plasmodium falciparum-infected erythrocytes of placental origin. Proc. Natl. Acad. Sci. USA 103: 13795-13800 [Abstract] [Full Text]  
  • Layez, C., Nogueira, P., Combes, V., Costa, F. T. M., Juhan-Vague, I., da Silva, L. H. P., Gysin, J. (2005). Plasmodium falciparum rhoptry protein RSP2 triggers destruction of the erythroid lineage. Blood 106: 3632-3638 [Abstract] [Full Text]  
  • Baratin, M., Roetynck, S., Lepolard, C., Falk, C., Sawadogo, S., Uematsu, S., Akira, S., Ryffel, B., Tiraby, J.-G., Alexopoulou, L., Kirschning, C. J., Gysin, J., Vivier, E., Ugolini, S. (2005). Natural killer cell and macrophage cooperation in MyD88-dependent innate responses to Plasmodium falciparum. Proc. Natl. Acad. Sci. USA 102: 14747-14752 [Abstract] [Full Text]  
  • Muthusamy, A., Achur, R. N., Bhavanandan, V. P., Fouda, G. G., Taylor, D. W., Gowda, D. C. (2004). Plasmodium falciparum-Infected Erythrocytes Adhere Both in the Intervillous Space and on the Villous Surface of Human Placenta by Binding to the Low-Sulfated Chondroitin Sulfate Proteoglycan Receptor. Am. J. Pathol. 164: 2013-2025 [Abstract] [Full Text]  
  • Douki, J.-B. L., Sterkers, Y., Lepolard, C., Traore, B., Costa, F. T. M., Scherf, A., Gysin, J. (2003). Adhesion of normal and Plasmodium falciparum ring-infected erythrocytes to endothelial cells and the placenta involves the rhoptry-derived ring surface protein-2. Blood 101: 5025-5032 [Abstract] [Full Text]  
  • Agbor-Enoh, S. T., Achur, R. N., Valiyaveettil, M., Leke, R., Taylor, D. W., Gowda, D. C. (2003). Chondroitin Sulfate Proteoglycan Expression and Binding of Plasmodium falciparum-Infected Erythrocytes in the Human Placenta during Pregnancy. Infect. Immun. 71: 2455-2461 [Abstract] [Full Text]  
  • Achur, R. N., Valiyaveettil, M., Gowda, D. C. (2003). The Low Sulfated Chondroitin Sulfate Proteoglycans of Human Placenta Have Sulfate Group-clustered Domains That Can Efficiently Bind Plasmodium falciparum-infected Erythrocytes. J. Biol. Chem. 278: 11705-11713 [Abstract] [Full Text]  
  • Kisilevsky, R., Crandall, I., Szarek, W. A., Bhat, S., Tan, C., Boudreau, L., Kain, K. C. (2002). Short-Chain Aliphatic Polysulfonates Inhibit the Entry of Plasmodium into Red Blood Cells. Antimicrob. Agents Chemother. 46: 2619-2626 [Abstract] [Full Text]  
  • Lekana Douki, J.-B., Traore, B., Costa, F. T. M., Fusai, T., Pouvelle, B., Sterkers, Y., Scherf, A., Gysin, J. (2002). Sequestration of Plasmodium falciparum-infected erythrocytes to chondroitin sulfate A, a receptor for maternal malaria: monoclonal antibodies against the native parasite ligand reveal pan-reactive epitopes in placental isolates. Blood 100: 1478-1483 [Abstract] [Full Text]  
  • O'Neil-Dunne, I., Achur, R. N., Agbor-Enoh, S. T., Valiyaveettil, M., Naik, R. S., Ockenhouse, C. F., Zhou, A., Megnekou, R., Leke, R., Taylor, D. W., Gowda, D. C. (2001). Gravidity-Dependent Production of Antibodies That Inhibit Binding of Plasmodium falciparum-Infected Erythrocytes to Placental Chondroitin Sulfate Proteoglycan during Pregnancy. Infect. Immun. 69: 7487-7492 [Abstract] [Full Text]  
  • Ricke, C. H., Staalsoe, T., Koram, K., Akanmori, B. D., Riley, E. M., Theander, T. G., Hviid, L. (2000). Plasma Antibodies from Malaria-Exposed Pregnant Women Recognize Variant Surface Antigens on Plasmodium falciparum-Infected Erythrocytes in a Parity-Dependent Manner and Block Parasite Adhesion to Chondroitin Sulfate A. J. Immunol. 165: 3309-3316 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»