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Infection and Immunity, December 1999, p. 6695-6697, Vol. 67, No. 12
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Constitutive and Inducible Expression of Green Fluorescent Protein in Brucella suis

Stephan Köhler,* Safia Ouahrani-Bettache, Marion Layssac, Jacques Teyssier, and Jean-Pierre Liautard

Institut National de la Santé et de la Recherche Médicale U-431, Université Montpellier II, F-34095 Montpellier, France

Received 6 July 1999/Returned for modification 26 August 1999/Accepted 9 September 1999

A gene fusion system based on plasmid pBBR1MCS and the expression of green fluorescent protein was developed for Brucella suis, allowing isolation of constitutive and inducible genes. Bacteria containing promoter fusions of chromosomal DNA to gfp were visualized by fluorescence microscopy and examined by flow cytometry. Twelve clones containing gene fragments induced inside J774 murine macrophages were isolated and further characterized.

* Corresponding author. Mailing address: INSERM U-431, Université Montpellier II, C.P. 100, Pl. E. Bataillon, 34095 Montpellier Cedex 05, France. Phone: 33 4 67 14 42 38. Fax: 33 4 67 14 33 38. E-mail: kohler{at}crit.univ-montp2.fr.

Infection and Immunity, December 1999, p. 6695-6697, Vol. 67, No. 12
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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