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Infection and Immunity, December 1999, p. 6695-6697, Vol. 67, No. 12
Institut National de la Santé et de la
Recherche Médicale U-431, Université Montpellier II,
F-34095 Montpellier, France
Received 6 July 1999/Returned for modification 26 August
1999/Accepted 9 September 1999
A gene fusion system based on plasmid pBBR1MCS and the expression
of green fluorescent protein was developed for Brucella suis, allowing isolation of constitutive and inducible genes. Bacteria containing promoter fusions of chromosomal DNA to
gfp were visualized by fluorescence microscopy and examined
by flow cytometry. Twelve clones containing gene fragments induced
inside J774 murine macrophages were isolated and further characterized.
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Constitutive and Inducible Expression of Green
Fluorescent Protein in Brucella suis
*
Corresponding author. Mailing address: INSERM U-431,
Université Montpellier II, C.P. 100, Pl. E. Bataillon, 34095 Montpellier Cedex 05, France. Phone: 33 4 67 14 42 38. Fax: 33 4 67 14 33 38. E-mail: kohler{at}crit.univ-montp2.fr.
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