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Infection and Immunity, December 1999, p. 6702-6706, Vol. 67, No. 12
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Analysis of Antibody Response to Invariable Regions of VlsE, the Variable Surface Antigen of Borrelia burgdorferi

Fang Ting Liang and Mario T. Philipp*

Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana 70433

Received 3 August 1999/Returned for modification 20 September 1999/Accepted 28 September 1999

VlsE, the variable surface antigen of Borrelia burgdorferi, consists of two invariable domains at the amino and carboxyl termini and one central variable domain. The latter contains six invariable regions, IR1 to IR6, and six variable regions. In the present study, the antigenicity of all of the invariable regions in B. burgdorferi-infected monkeys, humans, and mice was assessed by peptide-based enzyme-linked immunosorbent assays. Only one invariable region, IR6, was antigenic in all animals of the three host species. IR2 and IR4 were also antigenic in mice.

* Corresponding author. Mailing address: Tulane Regional Primate Research Center, Tulane University Medical Center, 18703 Three Rivers Rd., Covington, LA 70433. Phone: (504) 871-6221. Fax: (504) 871-6390. E-mail: philipp{at}tpc.tulane.edu.

Infection and Immunity, December 1999, p. 6702-6706, Vol. 67, No. 12
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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