This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goosney, D. L.
Right arrow Articles by Finlay, B. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goosney, D. L.
Right arrow Articles by Finlay, B. B.

 Previous Article  |  Next Article 

Infection and Immunity, February 1999, p. 490-495, Vol. 67, No. 2
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Enteropathogenic Escherichia coli Inhibits Phagocytosis

Danika L. Goosney, Jean Celli, Brendan Kenny,dagger and B. Brett Finlay*

Biotechnology Laboratory and Departments of Microbiology & Immunology and of Biochemistry & Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada

Received 16 March 1998/Returned for modification 27 May 1998/Accepted 24 November 1998

Enteropathogenic Escherichia coli (EPEC) interacts with intestinal epithelial cells, activating host signaling pathways leading to cytoskeletal rearrangements and ultimately diarrhea. In this study, we demonstrate that EPEC interacts with the macrophage-like cell line J774A.1 to inhibit phagocytosis by these cells. Antiphagocytic activity was also observed in cultured RAW macrophage-like cells upon EPEC infection. The EPEC antiphagocytic phenotype was dependent on the type III secretion pathway of EPEC and its secreted proteins, including EspA, EspB, and EspD. Intimin and Tir mutants displayed intermediate antiphagocytic activity, suggesting that intimate attachment mediated by intimin-Tir binding may also play a role in antiphagocytosis. Tyrosine dephosphorylation of several host proteins was observed following infection with secretion-competent EPEC but not with secretion-deficient mutants. Dephosphorylation was detectable 120 min after infection with EPEC, directly correlating with the onset of the antiphagocytic phenotype. Inhibition of protein tyrosine phosphatases by pervanadate treatment increased the number of intracellular wild-type EPEC organisms to levels seen with secretion-deficient mutants, suggesting that dephosphorylation events are linked to the antiphagocytic phenotype. No tyrosine phosphatase activity was detected with the EPEC-secreted proteins, suggesting that EPEC induces antiphagocytosis via a different mechanism than Yersinia species. Taken together, the present findings demonstrate a novel function for EPEC-secreted proteins in triggering macrophage protein tyrosine dephosphorylation and inhibition of phagocytosis.

* Corresponding author. Mailing address: Biotechnology Laboratory and Departments of Microbiology & Immunology and of Biochemistry & Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada. Phone: (604) 822-2210. Fax: (604) 822-9830. E-mail: bfinlay{at}unixg.ubc.ca.

dagger Present address: Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, Great Britain.

Infection and Immunity, February 1999, p. 490-495, Vol. 67, No. 2
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Brest, P., Betis, F., Cuburu, N., Selva, E., Herrant, M., Servin, A., Auberger, P., Hofman, P. (2004). Increased Rate of Apoptosis and Diminished Phagocytic Ability of Human Neutrophils Infected with Afa/Dr Diffusely Adhering Escherichia coli Strains. Infect. Immun. 72: 5741-5749 [Abstract] [Full Text]  
  • Segura, M., Gottschalk, M., Olivier, M. (2004). Encapsulated Streptococcus suis Inhibits Activation of Signaling Pathways Involved in Phagocytosis. Infect. Immun. 72: 5322-5330 [Abstract] [Full Text]  
  • Vallance, B. A., Deng, W., De Grado, M., Chan, C., Jacobson, K., Finlay, B. B. (2002). Modulation of Inducible Nitric Oxide Synthase Expression by the Attaching and Effacing Bacterial Pathogen Citrobacter rodentium in Infected Mice. Infect. Immun. 70: 6424-6435 [Abstract] [Full Text]  
  • Stevens, M. P., van Diemen, P. M., Frankel, G., Phillips, A. D., Wallis, T. S. (2002). Efa1 Influences Colonization of the Bovine Intestine by Shiga Toxin-Producing Escherichia coli Serotypes O5 and O111. Infect. Immun. 70: 5158-5166 [Abstract] [Full Text]  
  • Vallance, B. A., Deng, W., Knodler, L. A., Finlay, B. B. (2002). Mice Lacking T and B Lymphocytes Develop Transient Colitis and Crypt Hyperplasia yet Suffer Impaired Bacterial Clearance during Citrobacter rodentium Infection. Infect. Immun. 70: 2070-2081 [Abstract] [Full Text]  
  • Kazmierczak, B. I., Engel, J. N. (2002). Pseudomonas aeruginosa ExoT Acts In Vivo as a GTPase-Activating Protein for RhoA, Rac1, and Cdc42. Infect. Immun. 70: 2198-2205 [Abstract] [Full Text]  
  • Lindmark, H., Johansson, K. C., Stoven, S., Hultmark, D., Engstrom, Y., Soderhall, K. (2001). Enteric Bacteria Counteract Lipopolysaccharide Induction of Antimicrobial Peptide Genes. J. Immunol. 167: 6920-6923 [Abstract] [Full Text]  
  • Nougayrede, J.-P., Boury, M., Tasca, C., Marches, O., Milon, A., Oswald, E., De Rycke, J. (2001). Type III Secretion-Dependent Cell Cycle Block Caused in HeLa Cells by Enteropathogenic Escherichia coli O103. Infect. Immun. 69: 6785-6795 [Abstract] [Full Text]  
  • Glasser, A.-L., Boudeau, J., Barnich, N., Perruchot, M.-H., Colombel, J.-F., Darfeuille-Michaud, A. (2001). Adherent Invasive Escherichia coli Strains from Patients with Crohn's Disease Survive and Replicate within Macrophages without Inducing Host Cell Death. Infect. Immun. 69: 5529-5537 [Abstract] [Full Text]  
  • Wood, G. E., Dutro, S. M., Totten, P. A. (2001). Haemophilus ducreyi Inhibits Phagocytosis by U-937 Cells, a Human Macrophage-Like Cell Line. Infect. Immun. 69: 4726-4733 [Abstract] [Full Text]  
  • Hecht, G. (2001). Microbes and Microbial Toxins: Paradigms for Microbial-Mucosal Interactions: VII. Enteropathogenic Escherichia coli: physiological alterations from an extracellular position. Am. J. Physiol. Gastrointest. Liver Physiol. 281: G1-G7 [Abstract] [Full Text]  
  • Fiorentini, C., Falzano, L., Fabbri, A., Stringaro, A., Logozzi, M., Travaglione, S., Contamin, S., Arancia, G., Malorni, W., Fais, S. (2001). Activation of Rho GTPases by Cytotoxic Necrotizing Factor 1 Induces Macropinocytosis and Scavenging Activity in Epithelial Cells. Mol. Biol. Cell 12: 2061-2073 [Abstract] [Full Text]  
  • Ramarao, N., Meyer, T. F. (2001). Helicobacter pylori Resists Phagocytosis by Macrophages: Quantitative Assessment by Confocal Microscopy and Fluorescence-Activated Cell Sorting. Infect. Immun. 69: 2604-2611 [Abstract] [Full Text]  
  • Garrity-Ryan, L., Kazmierczak, B., Kowal, R., Comolli, J., Hauser, A., Engel, J. N. (2000). The Arginine Finger Domain of ExoT Contributes to Actin Cytoskeleton Disruption and Inhibition of Internalization of Pseudomonas aeruginosa by Epithelial Cells and Macrophages. Infect. Immun. 68: 7100-7113 [Abstract] [Full Text]  
  • Vallance, B. A., Finlay, B. B. (2000). Exploitation of host cells by enteropathogenic Escherichiacoli. Proc. Natl. Acad. Sci. USA 97: 8799-8806 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»