This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Favre-Bonte, S.
Right arrow Articles by Forestier, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Favre-Bonte, S.
Right arrow Articles by Forestier, C.

 Previous Article  |  Next Article 

Infection and Immunity, February 1999, p. 554-561, Vol. 67, No. 2
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Consequences of Reduction of Klebsiella pneumoniae Capsule Expression on Interactions of This Bacterium with Epithelial Cells

Sabine Favre-Bonte, Bernard Joly, and Christiane Forestier*

Laboratoire de Bactériologie, Faculté de Pharmacie, Université d'Auvergne-Clermont 1, 63001 Clermont-Ferrand Cedex, France

Received 27 April 1998/Returned for modification 6 July 1998/Accepted 19 October 1998

Most Klebsiella pneumoniae clinical isolates are fully encapsulated and adhere in vitro to intestinal cell lines with an aggregative pattern. In this study, the influence of the capsule on interactions with epithelial cells was investigated by creating an isogenic mutant defective in the synthesis of the capsule. Determination of the uronic acid content of bacterial extracts confirmed that the mutant did not produce capsular polysaccharides whereas, with the wild-type strain, the level of encapsulation was growth phase dependent and reached a maximum during the lag and early log phases. Assays performed with different epithelial cell lines, Int-407, A-549, and HEp-2, showed that the capsule-defective mutant demonstrated greater adhesion than did the wild-type strain and that the aggregative pattern was maintained, indicating that the capsule was not related to the adhesion phenotype. In contrast, when the mucus-producing HT-29-MTX cells were used, the encapsulated wild-type strain adhered more strongly than did the capsule-defective mutant. No invasion properties were observed with any of the capsular phenotypes or cell lines used. The K. pneumoniae adhesin CF29K was detected by Western blot analysis and enzyme-linked immunosorbent assay on the surface of transconjugants obtained after transfer of a conjugative plasmid harboring the CF29K-encoding genes into both the wild-type and the capsule-defective strains. The amounts of adhesin detected were greater in the capsule-defective background strain than in the wild-type encapsulated strain and were associated with an increase in the level of adhesion to Caco-2 cells. Moreover, RNA slot blot experiments showed that transcription of the adhesin-encoding gene was markedly increased in the capsule-defective mutant compared to the wild-type encapsulated background. These results suggest (i) that the capsule plays an active role during the initial steps of the pathogenesis by interacting with mucus-producing cells but is subsequently not required for the adhesin-related interaction with the epithelial cell surface and (ii) that the expression of the adhesin is modulated by the presence of a capsule at a transcriptional level.

* Corresponding author. Mailing address: Laboratoire de Bactériologie, Faculté de Pharmacie, Université d'Auvergne, 28 place H. Dunant, 63001 Clermont-Ferrand Cedex, France. Phone: (33) 4 73 60 80 19. Fax: (33) 4 73 27 74 94. E-mail: Christiane.forestier{at}u-clermont1.fr.

Infection and Immunity, February 1999, p. 554-561, Vol. 67, No. 2
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Shavkunov, K. S., Masulis, I. S., Tutukina, M. N., Deev, A. A., Ozoline, O. N. (2009). Gains and unexpected lessons from genome-scale promoter mapping. Nucleic Acids Res 0: gkp490v1-gkp490 [Abstract] [Full Text]  
  • Corcionivoschi, N., Clyne, M., Lyons, A., Elmi, A., Gundogdu, O., Wren, B. W., Dorrell, N., Karlyshev, A. V., Bourke, B. (2009). Campylobacter jejuni Cocultured with Epithelial Cells Reduces Surface Capsular Polysaccharide Expression. Infect. Immun. 77: 1959-1967 [Abstract] [Full Text]  
  • Regueiro, V., Moranta, D., Campos, M. A., Margareto, J., Garmendia, J., Bengoechea, J. A. (2009). Klebsiella pneumoniae Increases the Levels of Toll-Like Receptors 2 and 4 in Human Airway Epithelial Cells. Infect. Immun. 77: 714-724 [Abstract] [Full Text]  
  • Coudeyras, S., Nakusi, L., Charbonnel, N., Forestier, C. (2008). A Tripartite Efflux Pump Involved in Gastrointestinal Colonization by Klebsiella pneumoniae Confers a Tolerance Response to Inorganic Acid. Infect. Immun. 76: 4633-4641 [Abstract] [Full Text]  
  • Struve, C., Bojer, M., Krogfelt, K. A. (2008). Characterization of Klebsiella pneumoniae Type 1 Fimbriae by Detection of Phase Variation during Colonization and Infection and Impact on Virulence. Infect. Immun. 76: 4055-4065 [Abstract] [Full Text]  
  • Sahly, H., Navon-Venezia, S., Roesler, L., Hay, A., Carmeli, Y., Podschun, R., Hennequin, C., Forestier, C., Ofek, I. (2008). Extended-Spectrum {beta}-Lactamase Production Is Associated with an Increase in Cell Invasion and Expression of Fimbrial Adhesins in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 52: 3029-3034 [Abstract] [Full Text]  
  • Clermont, O., Lavollay, M., Vimont, S., Deschamps, C., Forestier, C., Branger, C., Denamur, E., Arlet, G. (2008). The CTX-M-15-producing Escherichia coli diffusing clone belongs to a highly virulent B2 phylogenetic subgroup. J Antimicrob Chemother 61: 1024-1028 [Abstract] [Full Text]  
  • Sahly, H., Keisari, Y., Crouch, E., Sharon, N., Ofek, I. (2008). Recognition of Bacterial Surface Polysaccharides by Lectins of the Innate Immune System and Its Contribution to Defense against Infection: the Case of Pulmonary Pathogens. Infect. Immun. 76: 1322-1332 [Full Text]  
  • Lawlor, M. S., O'Connor, C., Miller, V. L. (2007). Yersiniabactin Is a Virulence Factor for Klebsiella pneumoniae during Pulmonary Infection. Infect. Immun. 75: 1463-1472 [Abstract] [Full Text]  
  • Sukupolvi-Petty, S., Grass, S., StGeme, J. W. III (2006). The Haemophilus influenzae Type b hcsA and hcsB Gene Products Facilitate Transport of Capsular Polysaccharide across the Outer Membrane and Are Essential for Virulence. J. Bacteriol. 188: 3870-3877 [Abstract] [Full Text]  
  • Regueiro, V., Campos, M. A., Pons, J., Alberti, S., Bengoechea, J. A. (2006). The uptake of a Klebsiella pneumoniae capsule polysaccharide mutant triggers an inflammatory response by human airway epithelial cells. Microbiology 152: 555-566 [Abstract] [Full Text]  
  • Jenney, A. W., Clements, A., Farn, J. L., Wijburg, O. L., McGlinchey, A., Spelman, D. W., Pitt, T. L., Kaufmann, M. E., Liolios, L., Moloney, M. B., Wesselingh, S. L., Strugnell, R. A. (2006). Seroepidemiology of Klebsiella pneumoniae in an Australian Tertiary Hospital and Its Implications for Vaccine Development. J. Clin. Microbiol. 44: 102-107 [Abstract] [Full Text]  
  • Wu, J. H., Tsai, C. G. (2005). Infectivity of Hepatic Strain Klebsiella pneumoniae in Diabetic Mice. Exp. Biol. Med. 230: 757-761 [Abstract] [Full Text]  
  • Schembri, M. A., Blom, J., Krogfelt, K. A., Klemm, P. (2005). Capsule and Fimbria Interaction in Klebsiella pneumoniae. Infect. Immun. 73: 4626-4633 [Abstract] [Full Text]  
  • Balestrino, D., Haagensen, J. A. J., Rich, C., Forestier, C. (2005). Characterization of Type 2 Quorum Sensing in Klebsiella pneumoniae and Relationship with Biofilm Formation. J. Bacteriol. 187: 2870-2880 [Abstract] [Full Text]  
  • Schwan, W. R., Beck, M. T., Hultgren, S. J., Pinkner, J., Woolever, N. L., Larson, T. (2005). Down-Regulation of the kps Region 1 Capsular Assembly Operon following Attachment of Escherichia coli Type 1 Fimbriae to D-Mannose Receptors. Infect. Immun. 73: 1226-1231 [Abstract] [Full Text]  
  • Yi, K., Rasmussen, A. W., Gudlavalleti, S. K., Stephens, D. S., Stojiljkovic, I. (2004). Biofilm Formation by Neisseria meningitidis. Infect. Immun. 72: 6132-6138 [Abstract] [Full Text]  
  • Joseph, L. A., Wright, A. C. (2004). Expression of Vibrio vulnificus Capsular Polysaccharide Inhibits Biofilm Formation. J. Bacteriol. 186: 889-893 [Abstract] [Full Text]  
  • Sahly, H., Ofek, I., Podschun, R., Brade, H., He, Y., Ullmann, U., Crouch, E. (2002). Surfactant Protein D Binds Selectively to Klebsiella pneumoniae Lipopolysaccharides Containing Mannose-Rich O-Antigens. J. Immunol. 169: 3267-3274 [Abstract] [Full Text]  
  • Maroncle, N., Balestrino, D., Rich, C., Forestier, C. (2002). Identification of Klebsiella pneumoniae Genes Involved in Intestinal Colonization and Adhesion Using Signature-Tagged Mutagenesis. Infect. Immun. 70: 4729-4734 [Abstract] [Full Text]  
  • Cucarella, C., Tormo, M. A., Knecht, E., Amorena, B., Lasa, I., Foster, T. J., Penades, J. R. (2002). Expression of the Biofilm-Associated Protein Interferes with Host Protein Receptors of Staphylococcus aureus and Alters the Infective Process. Infect. Immun. 70: 3180-3186 [Abstract] [Full Text]  
  • Cortes, G., Alvarez, D., Saus, C., Alberti, S. (2002). Role of Lung Epithelial Cells in Defense against Klebsiella pneumoniae Pneumonia. Infect. Immun. 70: 1075-1080 [Abstract] [Full Text]  
  • Keisari, Y., Wang, H., Mesika, A., Matatov, R., Nissimov, L., Crouch, E., Ofek, I. (2001). Surfactant protein D-coated Klebsiella pneumoniae stimulates cytokine production in mononuclear phagocytes. J. Leukoc. Biol. 70: 135-141 [Abstract] [Full Text]  
  • Ofek, I., Mesika, A., Kalina, M., Keisari, Y., Podschun, R., Sahly, H., Chang, D., McGregor, D., Crouch, E. (2001). Surfactant Protein D Enhances Phagocytosis and Killing of Unencapsulated Phase Variants of Klebsiella pneumoniae. Infect. Immun. 69: 24-33 [Abstract] [Full Text]  
  • Sahly, H., Podschun, R., Oelschlaeger, T. A., Greiwe, M., Parolis, H., Hasty, D., Kekow, J., Ullmann, U., Ofek, I., Sela, S. (2000). Capsule Impedes Adhesion to and Invasion of Epithelial Cells by Klebsiella pneumoniae. Infect. Immun. 68: 6744-6749 [Abstract] [Full Text]  
  • Pohlmann-Dietze, P., Ulrich, M., Kiser, K. B., Doring, G., Lee, J. C., Fournier, J.-M., Botzenhart, K., Wolz, C. (2000). Adherence of Staphylococcus aureus to Endothelial Cells: Influence of Capsular Polysaccharide, Global Regulator agr, and Bacterial Growth Phase. Infect. Immun. 68: 4865-4871 [Abstract] [Full Text]  
  • Boyce, J. D., Adler, B. (2000). The Capsule Is a Virulence Determinant in the Pathogenesis of Pasteurella multocida M1404 (B:2). Infect. Immun. 68: 3463-3468 [Abstract] [Full Text]  
  • Favre-Bonte, S., Licht, T. R., Forestier, C., Krogfelt, K. A. (1999). Klebsiella pneumoniae Capsule Expression Is Necessary for Colonization of Large Intestines of Streptomycin-Treated Mice. Infect. Immun. 67: 6152-6156 [Abstract] [Full Text]  
  • Cornillon, S., Pech, E., Benghezal, M., Ravanel, K., Gaynor, E., Letourneur, F., Bruckert, F., Cosson, P. (2000). Phg1p Is a Nine-transmembrane Protein Superfamily Member Involved in Dictyostelium Adhesion and Phagocytosis. J. Biol. Chem. 275: 34287-34292 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»