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Infection and Immunity, March 1999, p. 1056-1062, Vol. 67, No. 3
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Role of Bordetella pertussis Virulence Factors in Adherence to Epithelial Cell Lines Derived from the Human Respiratory Tract

Bernard M. van den Berg,1,* Henry Beekhuizen,1 Rob J. L. Willems,2 Frits R. Mooi,2 and Ralph van Furth1

Department of Infectious Diseases, Leiden University Medical Center, Leiden,1 and Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, Bilthoven,2 The Netherlands

Received 25 September 1998/Returned for modification 4 December 1998/Accepted 11 December 1998

During colonization of the respiratory tract by Bordetella pertussis, virulence factors contribute to adherence of the bacterium to the respiratory tract epithelium. In the present study, we examined the roles of the virulence factors filamentous hemagglutinin (FHA), fimbriae, pertactin (Prn), and pertussis toxin (PT) in the adherence of B. pertussis to cells of the human bronchial epithelial cell line NCI-H292 and of the laryngeal epithelial cell line HEp-2. Using B. pertussis mutant strains and purified FHA, fimbriae, Prn, and PT, we demonstrated that both fimbriae and FHA are involved in the adhesion of B. pertussis to laryngeal epithelial cells, whereas only FHA is involved in the adherence to bronchial epithelial cells. For PT and Prn, no role as adhesion factor was found. However, purified PT bound to both bronchial and laryngeal cells and as such reduced the adherence of B. pertussis to these cells. These data may imply that fimbriae play a role in infection of only the laryngeal mucosa, while FHA is the major factor in colonization of the entire respiratory tract.

* Corresponding author. Mailing address: Dept. of Infectious Diseases, Leiden University Medical Center, Building 1, C5-P, P.O. Box 9600, 2300 RC Leiden, The Netherlands. Phone: 31-71-5262613. Fax: 31-71-5266758. E-mail: bvdberg{at}stad.dsl.nl.

Infection and Immunity, March 1999, p. 1056-1062, Vol. 67, No. 3
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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