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Infection and Immunity, March 1999, p. 1056-1062, Vol. 67, No. 3
Department of Infectious Diseases,
Received 25 September 1998/Returned for modification 4 December
1998/Accepted 11 December 1998
During colonization of the respiratory tract by Bordetella
pertussis, virulence factors contribute to adherence of the
bacterium to the respiratory tract epithelium. In the present study, we examined the roles of the virulence factors filamentous hemagglutinin (FHA), fimbriae, pertactin (Prn), and pertussis toxin (PT) in the
adherence of B. pertussis to cells of the human bronchial epithelial cell line NCI-H292 and of the laryngeal
epithelial cell line HEp-2. Using B. pertussis mutant
strains and purified FHA, fimbriae, Prn, and PT, we demonstrated that
both fimbriae and FHA are involved in the adhesion of B. pertussis to laryngeal epithelial cells, whereas only FHA is
involved in the adherence to bronchial epithelial cells. For PT and
Prn, no role as adhesion factor was found. However, purified PT bound
to both bronchial and laryngeal cells and as such reduced the adherence
of B. pertussis to these cells. These data may imply that
fimbriae play a role in infection of only the laryngeal mucosa, while
FHA is the major factor in colonization of the entire respiratory tract.
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Role of Bordetella pertussis Virulence Factors in
Adherence to Epithelial Cell Lines Derived from the Human
Respiratory Tract
*
Corresponding author. Mailing address: Dept. of
Infectious Diseases, Leiden University Medical Center, Building 1, C5-P, P.O. Box 9600, 2300 RC Leiden, The Netherlands. Phone:
31-71-5262613. Fax: 31-71-5266758. E-mail: bvdberg{at}stad.dsl.nl.
Infection and Immunity, March 1999, p. 1056-1062, Vol. 67, No. 3
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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