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Infection and Immunity, March 1999, p. 1172-1179, Vol. 67, No. 3
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Repertoire of Human Antibodies against the Polysaccharide Capsule of Streptococcus pneumoniae Serotype 6B

Yan Sun,1 Moon K. Park,2 Jihye Kim,2 Betty Diamond,3 Alan Solomon,4 and Moon H. Nahm1,*

Departments of Pediatrics, Pathology, and Medicine, University of Rochester, Rochester, New York 146421; Faculty of Biological Sciences, Chonbuk National University Chonju, 561-756, Korea2; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 104613; and Human Immunology and Cancer Program, Department of Medicine, University of Tennessee Medical Center/Graduate School of Medicine, Knoxville, Tennessee 379204

Received 12 June 1998/Returned for modification 5 August 1998/Accepted 1 October 1998

We examined the repertoire of antibodies to Streptococcus pneumoniae 6B capsular polysaccharide induced with the conventional polysaccharide vaccine in adults at the molecular level two ways. In the first, we purified from the sera of seven vaccinees antipneumococcal antibodies and determined their amino acid sequences. Their VH regions are mainly the products of VH3 family genes (candidate genes, 3-23, 3-07, 3-66, and 3-74), but the product of a VH1 family gene (candidate gene, 1-03) is occasionally used. All seven individuals have small amounts of polyclonal kappa + antibodies (Vkappa 1 to Vkappa 4 families), although kappa + antibodies are occasionally dominated by antibodies formed with the product of the A27 Vkappa gene. In contrast, lambda + anti-6B antibodies are dominated by the antibodies derived from one of 3 very similar Vlambda 2 family genes (candidate genes, 2c, 2e, and 2a2) and Clambda 1 gene product. The Vlambda 2+ antibodies express the 8.12 idiotype, which is expressed on anti-double-stranded-DNA antibodies. In one case, Vlambda is derived from a rarely expressed Vlambda gene, 10a. In the second approach, we studied a human hybridoma (Dob1) producing anti-6B antibody. Its VH region sequence is closely related to those of the 3-15 VH gene (88% nucleotide homology) and JH4 (92% homology). Its VL region is homologous to the 2a2 Vlambda 2 gene (91%) and Jlambda 1/Clambda 1. Taken together, the V region of human anti-6B antibodies is commonly formed by a VH3 and a Vlambda 2 family gene product.

* Corresponding author. Mailing address: Departments of Pediatrics, Pathology, and Medicine, University of Rochester, 601 Elmwood Ave., Box 777, Rochester, NY 14642-8777. Phone: (716) 275-7963. Fax: (716) 271-7512. E-mail: moon{at}vaccine.rochester.edu.

Infection and Immunity, March 1999, p. 1172-1179, Vol. 67, No. 3
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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