This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vijh, S.
Right arrow Articles by Pamer, E. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vijh, S.
Right arrow Articles by Pamer, E. G.
Right arrowPubmed/NCBI databases
Medline Plus Health Information
*Listeria Infections

 Previous Article  |  Next Article 

Infection and Immunity, March 1999, p. 1303-1309, Vol. 67, No. 3
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Noncompetitive Expansion of Cytotoxic T Lymphocytes Specific for Different Antigens during Bacterial Infection

Sujata Vijh,dagger Ingrid M. Pilip, and Eric G. Pamer*

Sections of Infectious Diseases and Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520

Received 10 September 1998/Returned for modification 26 October 1998/Accepted 3 December 1998

Listeria monocytogenes is an intracellular bacterium that elicits complex cytotoxic T-lymphocyte (CTL) responses in infected mice. The responses of CTL populations that differ in antigen specificity range in magnitude from large, dominant responses to small, subdominant responses. To test the hypothesis that dominant T-cell responses inhibit subdominant responses, we eliminated the two dominant epitopes of L. monocytogenes by anchor residue mutagenesis and measured the T-cell responses to the remaining subdominant epitopes. Surprisingly, the loss of dominant T-cell responses did not enhance subdominant responses. While mice immunized with bacteria lacking dominant epitopes developed L. monocytogenes-specific immunity, their ability to respond to dominant epitopes upon rechallenge with wild-type bacteria was markedly diminished. Recall responses in mice immunized with wild-type or epitope-deficient L. monocytogenes showed that antigen presentation during recall infection is sufficient for activating memory cells yet insufficient for optimal priming of naive T lymphocytes. Our findings suggest that T-cell priming to different epitopes during L. monocytogenes infection is not competitive. Rather, T-cell populations specific for different antigens but the same pathogen expand independently.

* Corresponding author. Mailing address: Section of Infectious Diseases, LCI 803, P.O. Box 208022, New Haven, CT 06520-8022. Phone: 203-785-3561. Fax: 203-785-3864. E-mail: eric.pamer{at}yale.edu.

dagger Present address: Henry M. Jackson Foundation for the Advancement of Military Medicine, Rockville, MD 20878.

Infection and Immunity, March 1999, p. 1303-1309, Vol. 67, No. 3
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Leong, M. L., Hampl, J., Liu, W., Mathur, S., Bahjat, K. S., Luckett, W., Dubensky, T. W. Jr., Brockstedt, D. G. (2009). Impact of Preexisting Vector-Specific Immunity on Vaccine Potency: Characterization of Listeria monocytogenes-Specific Humoral and Cellular Immunity in Humans and Modeling Studies Using Recombinant Vaccines in Mice. Infect. Immun. 77: 3958-3968 [Abstract] [Full Text]  
  • Tzelepis, F., de Alencar, B. C. G., Penido, M. L. O., Claser, C., Machado, A. V., Bruna-Romero, O., Gazzinelli, R. T., Rodrigues, M. M. (2008). Infection with Trypanosoma cruzi Restricts the Repertoire of Parasite-Specific CD8+ T Cells Leading to Immunodominance. J. Immunol. 180: 1737-1748 [Abstract] [Full Text]  
  • Vallbracht, S., Jessen, B., Mrusek, S., Enders, A., Collins, P. L., Ehl, S., Krempl, C. D. (2007). Influence of a Single Viral Epitope on T Cell Response and Disease After Infection of Mice with Respiratory Syncytial Virus. J. Immunol. 179: 8264-8273 [Abstract] [Full Text]  
  • Diaz-Guerra, E., Vernal, R., del Prete, M. J., Silva, A., Garcia-Sanz, J. A. (2007). CCL2 Inhibits the Apoptosis Program Induced by Growth Factor Deprivation, Rescuing Functional T Cells. J. Immunol. 179: 7352-7357 [Abstract] [Full Text]  
  • Lee, S., Miller, S. A., Wright, D. W., Rock, M. T., Crowe, J. E. Jr. (2007). Tissue-Specific Regulation of CD8+ T-Lymphocyte Immunodominance in Respiratory Syncytial Virus Infection. J. Virol. 81: 2349-2358 [Abstract] [Full Text]  
  • Lety, M.-A., Frehel, C., Raynaud, C., Dupuis, M., Charbit, A. (2006). Exploring the role of the CTL epitope region of listeriolysin O in the pathogenesis of Listeria monocytogenes.. Microbiology 152: 1287-1296 [Abstract] [Full Text]  
  • Ploss, A., Leiner, I., Pamer, E. G. (2005). Distinct Regulation of H2-M3-Restricted Memory T Cell Responses in Lymph Node and Spleen. J. Immunol. 175: 5998-6005 [Abstract] [Full Text]  
  • Ploss, A., Tran, A., Menet, E., Leiner, I., Pamer, E. G. (2005). Cross-Recognition of N-Formylmethionine Peptides Is a General Characteristic of H2-M3-Restricted CD8+ T Cells. Infect. Immun. 73: 4423-4426 [Abstract] [Full Text]  
  • Starks, H., Bruhn, K. W., Shen, H., Barry, R. A., Dubensky, T. W., Brockstedt, D., Hinrichs, D. J., Higgins, D. E., Miller, J. F., Giedlin, M., Bouwer, H. G. A. (2004). Listeria monocytogenes as a Vaccine Vector: Virulence Attenuation or Existing Antivector Immunity Does Not Diminish Therapeutic Efficacy. J. Immunol. 173: 420-427 [Abstract] [Full Text]  
  • Wong, P., Lara-Tejero, M., Ploss, A., Leiner, I., Pamer, E. G. (2004). Rapid Development of T Cell Memory. J. Immunol. 172: 7239-7245 [Abstract] [Full Text]  
  • Ploss, A., Lauvau, G., Contos, B., Kerksiek, K. M., Guirnalda, P. D., Leiner, I., Lenz, L. L., Bevan, M. J., Pamer, E. G. (2003). Promiscuity of MHC Class Ib-Restricted T Cell Responses. J. Immunol. 171: 5948-5955 [Abstract] [Full Text]  
  • Tvinnereim, A. R., Hamilton, S. E., Harty, J. T. (2002). CD8+-T-Cell Response to Secreted and Nonsecreted Antigens Delivered by Recombinant Listeria monocytogenes during Secondary Infection. Infect. Immun. 70: 153-162 [Abstract] [Full Text]  
  • Lauvau, G., Vijh, S., Kong, P., Horng, T., Kerksiek, K., Serbina, N., Tuma, R. A., Pamer, E. G. (2001). Priming of Memory But Not Effector CD8 T Cells by a Killed Bacterial Vaccine. Science 294: 1735-1739 [Abstract] [Full Text]  
  • De Boer, R. J., Oprea, M., Antia, R., Murali-Krishna, K., Ahmed, R., Perelson, A. S. (2001). Recruitment Times, Proliferation, and Apoptosis Rates during the CD8+ T-Cell Response to Lymphocytic Choriomeningitis Virus. J. Virol. 75: 10663-10669 [Abstract] [Full Text]  
  • Yamada, T., Uchiyama, H., Nagata, T., Uchijima, M., Suda, T., Chida, K., Nakamura, H., Koide, Y. (2001). Protective Cytotoxic T Lymphocyte Responses Induced by DNA Immunization against Immunodominant and Subdominant Epitopes of Listeria monocytogenes Are Noncompetitive. Infect. Immun. 69: 3427-3430 [Abstract] [Full Text]  
  • Busch, D. H., Kerksiek, K. M., Pamer, E. G. (2000). Differing Roles of Inflammation and Antigen in T Cell Proliferation and Memory Generation. J. Immunol. 164: 4063-4070 [Abstract] [Full Text]  
  • Tvinnereim, A. R., Harty, J. T. (2000). CD8+ T-Cell Priming against a Nonsecreted Listeria monocytogenes Antigen Is Independent of the Antimicrobial Activities of Gamma Interferon. Infect. Immun. 68: 2196-2204 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»